High-throughput axial MALDI-TOF MS using a 2-kHz repetition rate laser

E. Moskovets, J. Preisler, H. S. Chen, T. Rejtar, V. Andreev, B. L. Karger

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

A high-throughput axial MALDI-TOF mass spectrometer utilizing a laser with a 2-kHz pulse repetition-rate was constructed and tested. This fast mass spectrometer provided a data acquisition rate 10 times faster than a commercially available (200 Hz) axial mass spectrometer, while maintaining comparable limits of detection (200 amol of Glu fib peptide). Mass resolution, only slightly less than the commercial instrument (10 000 vs 14 000), was sufficient for baseline resolution of isotopic clusters of peptides with m/z <2700. A new method of mass calibration, which combined a limited number of internal and external standards, provided the same 15 ppm mass accuracy over the entire sample plate on either instrument. Implementing the 2-kHz laser required a faster data acquisition system and high-voltage pulse electronics, together with a novel strategy for rapid sample plate movements during acquisition, to achieve a sample analysis rate of up to 2 spots/s (with 800 shots/spot). The overall performance of the fast MALDI-TOF MS instrument was demonstrated by the acquisition, in 12 min, of an LC-MS data set from a plate of 625 fractions collected during LC separation of an 16O/18O differentially labeled proteomic sample of a tryptic digest of an E. coli lysate.

Original languageEnglish
Pages (from-to)912-919
Number of pages8
JournalAnalytical Chemistry
Volume78
Issue number3
DOIs
StatePublished - Feb 1 2006

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Mass spectrometers
Throughput
Lasers
Data acquisition
Pulse repetition rate
Peptides
Escherichia coli
Electronic equipment
Calibration
Electric potential

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Moskovets, E., Preisler, J., Chen, H. S., Rejtar, T., Andreev, V., & Karger, B. L. (2006). High-throughput axial MALDI-TOF MS using a 2-kHz repetition rate laser. Analytical Chemistry, 78(3), 912-919. https://doi.org/10.1021/ac051393t

High-throughput axial MALDI-TOF MS using a 2-kHz repetition rate laser. / Moskovets, E.; Preisler, J.; Chen, H. S.; Rejtar, T.; Andreev, V.; Karger, B. L.

In: Analytical Chemistry, Vol. 78, No. 3, 01.02.2006, p. 912-919.

Research output: Contribution to journalArticle

Moskovets, E, Preisler, J, Chen, HS, Rejtar, T, Andreev, V & Karger, BL 2006, 'High-throughput axial MALDI-TOF MS using a 2-kHz repetition rate laser', Analytical Chemistry, vol. 78, no. 3, pp. 912-919. https://doi.org/10.1021/ac051393t
Moskovets E, Preisler J, Chen HS, Rejtar T, Andreev V, Karger BL. High-throughput axial MALDI-TOF MS using a 2-kHz repetition rate laser. Analytical Chemistry. 2006 Feb 1;78(3):912-919. https://doi.org/10.1021/ac051393t
Moskovets, E. ; Preisler, J. ; Chen, H. S. ; Rejtar, T. ; Andreev, V. ; Karger, B. L. / High-throughput axial MALDI-TOF MS using a 2-kHz repetition rate laser. In: Analytical Chemistry. 2006 ; Vol. 78, No. 3. pp. 912-919.
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