Abstract
Spinal cord injury (SCI) causes male infertility, with low sperm motility the major long-term cause. It has been suggested in previous studies that some seminal components may be responsible for the pathological asthenozoospermia. It is hypothesized that platelet-activating factor (PAF) acetylhydrolase (PAFah), which originates in the epididymis and other accessory sexual glands, may be a causative factor. This enzyme catalyzes PAF to acetate and biologically inactive lyso-PAF. PAF is well recognized to be an important phospholipid mediator that stimulates sperm motility and enhances sperm capacitation and fertilization. The present study was designed to analyze differences in PAFah activity in semen of men with SCI and age-matched healthy men. PAFah assay reagent kits were used to measure enzymatic activity by monitoring the production rates of 4-nitrophenol on a spectrophotometer during a given interval. The results showed that subjects with SCI had a higher concentration of PAFah than men in the control group (P < .001). A statistically significant negative correlation was found between enzymatic activity and sperm motility (r2 = 0.8449; P < .001). Further studies will determine whether seminal vesicle dysfunction in men with SCI leads to abnormal PAFah activity, resulting in low sperm motility.
| Original language | English |
|---|---|
| Pages (from-to) | 429-433 |
| Number of pages | 5 |
| Journal | Journal of Andrology |
| Volume | 27 |
| Issue number | 3 |
| DOIs | |
| State | Published - May 1 2006 |
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Keywords
- Asthenozoospermia
- Infertility
- SCI
- Semen
- Sperm
ASJC Scopus subject areas
- Endocrinology
Cite this
High seminal platelet-activating factor acetylhydrolase activity in men with spinal cord injury. / Zhu, Jiuming; Brackett, Nancy; Aballa, Teodoro C.; Lynne, Charles M; Witt, Michael A.; Kort, Hilton I.; Roudebush, William E.
In: Journal of Andrology, Vol. 27, No. 3, 01.05.2006, p. 429-433.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - High seminal platelet-activating factor acetylhydrolase activity in men with spinal cord injury
AU - Zhu, Jiuming
AU - Brackett, Nancy
AU - Aballa, Teodoro C.
AU - Lynne, Charles M
AU - Witt, Michael A.
AU - Kort, Hilton I.
AU - Roudebush, William E.
PY - 2006/5/1
Y1 - 2006/5/1
N2 - Spinal cord injury (SCI) causes male infertility, with low sperm motility the major long-term cause. It has been suggested in previous studies that some seminal components may be responsible for the pathological asthenozoospermia. It is hypothesized that platelet-activating factor (PAF) acetylhydrolase (PAFah), which originates in the epididymis and other accessory sexual glands, may be a causative factor. This enzyme catalyzes PAF to acetate and biologically inactive lyso-PAF. PAF is well recognized to be an important phospholipid mediator that stimulates sperm motility and enhances sperm capacitation and fertilization. The present study was designed to analyze differences in PAFah activity in semen of men with SCI and age-matched healthy men. PAFah assay reagent kits were used to measure enzymatic activity by monitoring the production rates of 4-nitrophenol on a spectrophotometer during a given interval. The results showed that subjects with SCI had a higher concentration of PAFah than men in the control group (P < .001). A statistically significant negative correlation was found between enzymatic activity and sperm motility (r2 = 0.8449; P < .001). Further studies will determine whether seminal vesicle dysfunction in men with SCI leads to abnormal PAFah activity, resulting in low sperm motility.
AB - Spinal cord injury (SCI) causes male infertility, with low sperm motility the major long-term cause. It has been suggested in previous studies that some seminal components may be responsible for the pathological asthenozoospermia. It is hypothesized that platelet-activating factor (PAF) acetylhydrolase (PAFah), which originates in the epididymis and other accessory sexual glands, may be a causative factor. This enzyme catalyzes PAF to acetate and biologically inactive lyso-PAF. PAF is well recognized to be an important phospholipid mediator that stimulates sperm motility and enhances sperm capacitation and fertilization. The present study was designed to analyze differences in PAFah activity in semen of men with SCI and age-matched healthy men. PAFah assay reagent kits were used to measure enzymatic activity by monitoring the production rates of 4-nitrophenol on a spectrophotometer during a given interval. The results showed that subjects with SCI had a higher concentration of PAFah than men in the control group (P < .001). A statistically significant negative correlation was found between enzymatic activity and sperm motility (r2 = 0.8449; P < .001). Further studies will determine whether seminal vesicle dysfunction in men with SCI leads to abnormal PAFah activity, resulting in low sperm motility.
KW - Asthenozoospermia
KW - Infertility
KW - SCI
KW - Semen
KW - Sperm
UR - http://www.scopus.com/inward/record.url?scp=33646364331&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33646364331&partnerID=8YFLogxK
U2 - 10.2164/jandrol.05159
DO - 10.2164/jandrol.05159
M3 - Article
C2 - 16339448
AN - SCOPUS:33646364331
VL - 27
SP - 429
EP - 433
JO - Journal of Andrology
JF - Journal of Andrology
SN - 0196-3635
IS - 3
ER -