High seminal platelet-activating factor acetylhydrolase activity in men with spinal cord injury

Jiuming Zhu, Nancy Brackett, Teodoro C. Aballa, Charles M Lynne, Michael A. Witt, Hilton I. Kort, William E. Roudebush

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Spinal cord injury (SCI) causes male infertility, with low sperm motility the major long-term cause. It has been suggested in previous studies that some seminal components may be responsible for the pathological asthenozoospermia. It is hypothesized that platelet-activating factor (PAF) acetylhydrolase (PAFah), which originates in the epididymis and other accessory sexual glands, may be a causative factor. This enzyme catalyzes PAF to acetate and biologically inactive lyso-PAF. PAF is well recognized to be an important phospholipid mediator that stimulates sperm motility and enhances sperm capacitation and fertilization. The present study was designed to analyze differences in PAFah activity in semen of men with SCI and age-matched healthy men. PAFah assay reagent kits were used to measure enzymatic activity by monitoring the production rates of 4-nitrophenol on a spectrophotometer during a given interval. The results showed that subjects with SCI had a higher concentration of PAFah than men in the control group (P < .001). A statistically significant negative correlation was found between enzymatic activity and sperm motility (r2 = 0.8449; P < .001). Further studies will determine whether seminal vesicle dysfunction in men with SCI leads to abnormal PAFah activity, resulting in low sperm motility.

Original languageEnglish
Pages (from-to)429-433
Number of pages5
JournalJournal of Andrology
Volume27
Issue number3
DOIs
StatePublished - May 1 2006

Fingerprint

1-Alkyl-2-acetylglycerophosphocholine Esterase
Sperm Motility
Platelet Activating Factor
Spinal Cord Injuries
Asthenozoospermia
Sperm Capacitation
Seminal Vesicles
Epididymis
Male Infertility
Semen
Fertilization
Phospholipids
Acetates
Control Groups
Enzymes

Keywords

  • Asthenozoospermia
  • Infertility
  • SCI
  • Semen
  • Sperm

ASJC Scopus subject areas

  • Endocrinology

Cite this

Zhu, J., Brackett, N., Aballa, T. C., Lynne, C. M., Witt, M. A., Kort, H. I., & Roudebush, W. E. (2006). High seminal platelet-activating factor acetylhydrolase activity in men with spinal cord injury. Journal of Andrology, 27(3), 429-433. https://doi.org/10.2164/jandrol.05159

High seminal platelet-activating factor acetylhydrolase activity in men with spinal cord injury. / Zhu, Jiuming; Brackett, Nancy; Aballa, Teodoro C.; Lynne, Charles M; Witt, Michael A.; Kort, Hilton I.; Roudebush, William E.

In: Journal of Andrology, Vol. 27, No. 3, 01.05.2006, p. 429-433.

Research output: Contribution to journalArticle

Zhu, Jiuming ; Brackett, Nancy ; Aballa, Teodoro C. ; Lynne, Charles M ; Witt, Michael A. ; Kort, Hilton I. ; Roudebush, William E. / High seminal platelet-activating factor acetylhydrolase activity in men with spinal cord injury. In: Journal of Andrology. 2006 ; Vol. 27, No. 3. pp. 429-433.
@article{55f2d9c803184988b1f5d573ea924cda,
title = "High seminal platelet-activating factor acetylhydrolase activity in men with spinal cord injury",
abstract = "Spinal cord injury (SCI) causes male infertility, with low sperm motility the major long-term cause. It has been suggested in previous studies that some seminal components may be responsible for the pathological asthenozoospermia. It is hypothesized that platelet-activating factor (PAF) acetylhydrolase (PAFah), which originates in the epididymis and other accessory sexual glands, may be a causative factor. This enzyme catalyzes PAF to acetate and biologically inactive lyso-PAF. PAF is well recognized to be an important phospholipid mediator that stimulates sperm motility and enhances sperm capacitation and fertilization. The present study was designed to analyze differences in PAFah activity in semen of men with SCI and age-matched healthy men. PAFah assay reagent kits were used to measure enzymatic activity by monitoring the production rates of 4-nitrophenol on a spectrophotometer during a given interval. The results showed that subjects with SCI had a higher concentration of PAFah than men in the control group (P < .001). A statistically significant negative correlation was found between enzymatic activity and sperm motility (r2 = 0.8449; P < .001). Further studies will determine whether seminal vesicle dysfunction in men with SCI leads to abnormal PAFah activity, resulting in low sperm motility.",
keywords = "Asthenozoospermia, Infertility, SCI, Semen, Sperm",
author = "Jiuming Zhu and Nancy Brackett and Aballa, {Teodoro C.} and Lynne, {Charles M} and Witt, {Michael A.} and Kort, {Hilton I.} and Roudebush, {William E.}",
year = "2006",
month = "5",
day = "1",
doi = "10.2164/jandrol.05159",
language = "English",
volume = "27",
pages = "429--433",
journal = "Journal of Andrology",
issn = "0196-3635",
publisher = "American Society of Andrology Inc.",
number = "3",

}

TY - JOUR

T1 - High seminal platelet-activating factor acetylhydrolase activity in men with spinal cord injury

AU - Zhu, Jiuming

AU - Brackett, Nancy

AU - Aballa, Teodoro C.

AU - Lynne, Charles M

AU - Witt, Michael A.

AU - Kort, Hilton I.

AU - Roudebush, William E.

PY - 2006/5/1

Y1 - 2006/5/1

N2 - Spinal cord injury (SCI) causes male infertility, with low sperm motility the major long-term cause. It has been suggested in previous studies that some seminal components may be responsible for the pathological asthenozoospermia. It is hypothesized that platelet-activating factor (PAF) acetylhydrolase (PAFah), which originates in the epididymis and other accessory sexual glands, may be a causative factor. This enzyme catalyzes PAF to acetate and biologically inactive lyso-PAF. PAF is well recognized to be an important phospholipid mediator that stimulates sperm motility and enhances sperm capacitation and fertilization. The present study was designed to analyze differences in PAFah activity in semen of men with SCI and age-matched healthy men. PAFah assay reagent kits were used to measure enzymatic activity by monitoring the production rates of 4-nitrophenol on a spectrophotometer during a given interval. The results showed that subjects with SCI had a higher concentration of PAFah than men in the control group (P < .001). A statistically significant negative correlation was found between enzymatic activity and sperm motility (r2 = 0.8449; P < .001). Further studies will determine whether seminal vesicle dysfunction in men with SCI leads to abnormal PAFah activity, resulting in low sperm motility.

AB - Spinal cord injury (SCI) causes male infertility, with low sperm motility the major long-term cause. It has been suggested in previous studies that some seminal components may be responsible for the pathological asthenozoospermia. It is hypothesized that platelet-activating factor (PAF) acetylhydrolase (PAFah), which originates in the epididymis and other accessory sexual glands, may be a causative factor. This enzyme catalyzes PAF to acetate and biologically inactive lyso-PAF. PAF is well recognized to be an important phospholipid mediator that stimulates sperm motility and enhances sperm capacitation and fertilization. The present study was designed to analyze differences in PAFah activity in semen of men with SCI and age-matched healthy men. PAFah assay reagent kits were used to measure enzymatic activity by monitoring the production rates of 4-nitrophenol on a spectrophotometer during a given interval. The results showed that subjects with SCI had a higher concentration of PAFah than men in the control group (P < .001). A statistically significant negative correlation was found between enzymatic activity and sperm motility (r2 = 0.8449; P < .001). Further studies will determine whether seminal vesicle dysfunction in men with SCI leads to abnormal PAFah activity, resulting in low sperm motility.

KW - Asthenozoospermia

KW - Infertility

KW - SCI

KW - Semen

KW - Sperm

UR - http://www.scopus.com/inward/record.url?scp=33646364331&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33646364331&partnerID=8YFLogxK

U2 - 10.2164/jandrol.05159

DO - 10.2164/jandrol.05159

M3 - Article

C2 - 16339448

AN - SCOPUS:33646364331

VL - 27

SP - 429

EP - 433

JO - Journal of Andrology

JF - Journal of Andrology

SN - 0196-3635

IS - 3

ER -