High-risk human papillomavirus DNA detected in primary squamous cell carcinoma of urinary bladder

Jennifer Chapman-Fredricks, Maureen Cioffi-Lavina, Molly A. Accola, William M. Rehrauer, Monica Garcia-Buitrago, Carmen Gomez-Fernandez, Parvin Ganjei-Azar, Merce Jorda

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Context.-We reported previously that more than onethird (37%) of primary bladder squamous cell carcinomas (SCCs) demonstrate diffuse p16 immunoreactivity independent of gender. This observation made us question whether p16 overexpression in bladder carcinoma is due to human papillomavirus (HPV)-dependent mechanisms. Objective.-To determine whether the presence of highrisk HPV (HR-HPV) DNA could be detected in these tumor cells. Design.-Fourteen cases of primary bladder SCC, which were positive for p16 by immunohistochemistry, were probed for the detection of HR-HPV by in situ hybridization and the signal amplification Invader assay. Samples positive for detection of HR-HPV by Invader assay were amplified by using HR-HPV type-specific primers, and amplification products were DNA sequenced. Results.-Detection of HR-HPV by the in situ hybridization method was negative in all cases (0 of 14). However, in 3 of 14 cases (21.4%), the presence of HRHPV DNA was detected with the Cervista HPV HR Invader assay, which was followed by identification of genotype. All positive cases were confirmed by using HR-HPV typespecific amplification followed by DNA sequencing. Identified HR-HPV genotypes included HPV 16 (2 cases) and HPV 35 (1 case). Conclusions.-High-risk HPV DNA is detectable in a subset of primary bladder SCCs. Based on the welldocumented carcinogenic potential of HR-HPV, there is a necessity for additional studies to investigate the role of HR-HPV in bladder carcinogenesis.

Original languageEnglish
Pages (from-to)1088-1093
Number of pages6
JournalArchives of Pathology and Laboratory Medicine
Volume137
Issue number8
DOIs
StatePublished - Aug 1 2013

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Squamous Cell Carcinoma
Urinary Bladder
DNA
In Situ Hybridization
Genotype
Human papillomavirus 16
DNA Sequence Analysis
Carcinogenesis
Immunohistochemistry
Carcinoma
Neoplasms

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medical Laboratory Technology

Cite this

High-risk human papillomavirus DNA detected in primary squamous cell carcinoma of urinary bladder. / Chapman-Fredricks, Jennifer; Cioffi-Lavina, Maureen; Accola, Molly A.; Rehrauer, William M.; Garcia-Buitrago, Monica; Gomez-Fernandez, Carmen; Ganjei-Azar, Parvin; Jorda, Merce.

In: Archives of Pathology and Laboratory Medicine, Vol. 137, No. 8, 01.08.2013, p. 1088-1093.

Research output: Contribution to journalArticle

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abstract = "Context.-We reported previously that more than onethird (37{\%}) of primary bladder squamous cell carcinomas (SCCs) demonstrate diffuse p16 immunoreactivity independent of gender. This observation made us question whether p16 overexpression in bladder carcinoma is due to human papillomavirus (HPV)-dependent mechanisms. Objective.-To determine whether the presence of highrisk HPV (HR-HPV) DNA could be detected in these tumor cells. Design.-Fourteen cases of primary bladder SCC, which were positive for p16 by immunohistochemistry, were probed for the detection of HR-HPV by in situ hybridization and the signal amplification Invader assay. Samples positive for detection of HR-HPV by Invader assay were amplified by using HR-HPV type-specific primers, and amplification products were DNA sequenced. Results.-Detection of HR-HPV by the in situ hybridization method was negative in all cases (0 of 14). However, in 3 of 14 cases (21.4{\%}), the presence of HRHPV DNA was detected with the Cervista HPV HR Invader assay, which was followed by identification of genotype. All positive cases were confirmed by using HR-HPV typespecific amplification followed by DNA sequencing. Identified HR-HPV genotypes included HPV 16 (2 cases) and HPV 35 (1 case). Conclusions.-High-risk HPV DNA is detectable in a subset of primary bladder SCCs. Based on the welldocumented carcinogenic potential of HR-HPV, there is a necessity for additional studies to investigate the role of HR-HPV in bladder carcinogenesis.",
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