Abstract
Context.-We reported previously that more than onethird (37%) of primary bladder squamous cell carcinomas (SCCs) demonstrate diffuse p16 immunoreactivity independent of gender. This observation made us question whether p16 overexpression in bladder carcinoma is due to human papillomavirus (HPV)-dependent mechanisms. Objective.-To determine whether the presence of highrisk HPV (HR-HPV) DNA could be detected in these tumor cells. Design.-Fourteen cases of primary bladder SCC, which were positive for p16 by immunohistochemistry, were probed for the detection of HR-HPV by in situ hybridization and the signal amplification Invader assay. Samples positive for detection of HR-HPV by Invader assay were amplified by using HR-HPV type-specific primers, and amplification products were DNA sequenced. Results.-Detection of HR-HPV by the in situ hybridization method was negative in all cases (0 of 14). However, in 3 of 14 cases (21.4%), the presence of HRHPV DNA was detected with the Cervista HPV HR Invader assay, which was followed by identification of genotype. All positive cases were confirmed by using HR-HPV typespecific amplification followed by DNA sequencing. Identified HR-HPV genotypes included HPV 16 (2 cases) and HPV 35 (1 case). Conclusions.-High-risk HPV DNA is detectable in a subset of primary bladder SCCs. Based on the welldocumented carcinogenic potential of HR-HPV, there is a necessity for additional studies to investigate the role of HR-HPV in bladder carcinogenesis.
| Original language | English |
|---|---|
| Pages (from-to) | 1088-1093 |
| Number of pages | 6 |
| Journal | Archives of Pathology and Laboratory Medicine |
| Volume | 137 |
| Issue number | 8 |
| DOIs | |
| State | Published - Aug 1 2013 |
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ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Medical Laboratory Technology
Cite this
High-risk human papillomavirus DNA detected in primary squamous cell carcinoma of urinary bladder. / Chapman-Fredricks, Jennifer; Cioffi-Lavina, Maureen; Accola, Molly A.; Rehrauer, William M.; Garcia-Buitrago, Monica; Gomez-Fernandez, Carmen; Ganjei-Azar, Parvin; Jorda, Merce.
In: Archives of Pathology and Laboratory Medicine, Vol. 137, No. 8, 01.08.2013, p. 1088-1093.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - High-risk human papillomavirus DNA detected in primary squamous cell carcinoma of urinary bladder
AU - Chapman-Fredricks, Jennifer
AU - Cioffi-Lavina, Maureen
AU - Accola, Molly A.
AU - Rehrauer, William M.
AU - Garcia-Buitrago, Monica
AU - Gomez-Fernandez, Carmen
AU - Ganjei-Azar, Parvin
AU - Jorda, Merce
PY - 2013/8/1
Y1 - 2013/8/1
N2 - Context.-We reported previously that more than onethird (37%) of primary bladder squamous cell carcinomas (SCCs) demonstrate diffuse p16 immunoreactivity independent of gender. This observation made us question whether p16 overexpression in bladder carcinoma is due to human papillomavirus (HPV)-dependent mechanisms. Objective.-To determine whether the presence of highrisk HPV (HR-HPV) DNA could be detected in these tumor cells. Design.-Fourteen cases of primary bladder SCC, which were positive for p16 by immunohistochemistry, were probed for the detection of HR-HPV by in situ hybridization and the signal amplification Invader assay. Samples positive for detection of HR-HPV by Invader assay were amplified by using HR-HPV type-specific primers, and amplification products were DNA sequenced. Results.-Detection of HR-HPV by the in situ hybridization method was negative in all cases (0 of 14). However, in 3 of 14 cases (21.4%), the presence of HRHPV DNA was detected with the Cervista HPV HR Invader assay, which was followed by identification of genotype. All positive cases were confirmed by using HR-HPV typespecific amplification followed by DNA sequencing. Identified HR-HPV genotypes included HPV 16 (2 cases) and HPV 35 (1 case). Conclusions.-High-risk HPV DNA is detectable in a subset of primary bladder SCCs. Based on the welldocumented carcinogenic potential of HR-HPV, there is a necessity for additional studies to investigate the role of HR-HPV in bladder carcinogenesis.
AB - Context.-We reported previously that more than onethird (37%) of primary bladder squamous cell carcinomas (SCCs) demonstrate diffuse p16 immunoreactivity independent of gender. This observation made us question whether p16 overexpression in bladder carcinoma is due to human papillomavirus (HPV)-dependent mechanisms. Objective.-To determine whether the presence of highrisk HPV (HR-HPV) DNA could be detected in these tumor cells. Design.-Fourteen cases of primary bladder SCC, which were positive for p16 by immunohistochemistry, were probed for the detection of HR-HPV by in situ hybridization and the signal amplification Invader assay. Samples positive for detection of HR-HPV by Invader assay were amplified by using HR-HPV type-specific primers, and amplification products were DNA sequenced. Results.-Detection of HR-HPV by the in situ hybridization method was negative in all cases (0 of 14). However, in 3 of 14 cases (21.4%), the presence of HRHPV DNA was detected with the Cervista HPV HR Invader assay, which was followed by identification of genotype. All positive cases were confirmed by using HR-HPV typespecific amplification followed by DNA sequencing. Identified HR-HPV genotypes included HPV 16 (2 cases) and HPV 35 (1 case). Conclusions.-High-risk HPV DNA is detectable in a subset of primary bladder SCCs. Based on the welldocumented carcinogenic potential of HR-HPV, there is a necessity for additional studies to investigate the role of HR-HPV in bladder carcinogenesis.
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UR - http://www.scopus.com/inward/citedby.url?scp=84881077358&partnerID=8YFLogxK
U2 - 10.5858/arpa.2012-0122-OA
DO - 10.5858/arpa.2012-0122-OA
M3 - Article
C2 - 23899065
AN - SCOPUS:84881077358
VL - 137
SP - 1088
EP - 1093
JO - Archives of Pathology and Laboratory Medicine
JF - Archives of Pathology and Laboratory Medicine
SN - 0003-9985
IS - 8
ER -