High-resolution optical coherence tomography-guided donor tissue preparation for descemet membrane endothelial keratoplasty using the reverse big bubble technique

Florence Cabot, Vardhaman P. Kankariya, Marco Ruggeri, Sonia H Yoo, Pravin K. Vaddavalli, Jean-Marie A Parel, George D. Kymionis

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

PURPOSE:: The aim of this study was to assess the feasibility of high-resolution spectral domain optical coherence tomography (HR-SDOCT) to guide donor tissue preparation in Descemet membrane endothelial keratoplasty using the reverse big bubble technique. METHODS:: Three corneoscleral discs were included in this ex vivo experimental study. A 27-G cannula was introduced into each cornea at the periphery by 3 different surgeons. Each surgeon attempted to achieve the ideal depth (pre-Descemetic plane) of the tip of the cannula for air injection to produce the reverse big bubble to separate the Descemet membrane (DM) from the posterior stroma. A supine optical coherence tomography system built at the Ophthalmic Biophysics Center of the Bascom Palmer Eye Institute was used to estimate in real-time the depth reached by the tip of the cannula in the posterior stroma during tissue preparation. RESULTS:: After air injection, 1 successful big bubble was obtained, while each of the other corneoscleral discs had intrastromal emphysema and DM perforation. On HR-SDOCT evaluations, artifacts were noticed at the tip of the cannula. The successful big bubble demonstrated the separation of the DM and the stroma without intrastromal hyperreflectivity. Emphysema was visualized on the HR-SDOCT as a hyperdense intrastromal area shadowing the posterior structures of the anterior chamber. CONCLUSIONS:: The HR-SDOCT-guided reverse big bubble technique may be a useful method to prepare donor tissue in Descemet membrane endothelial keratoplasty. Further improvements in high-resolution optical coherence tomography technology are needed this promising technique.

Original languageEnglish
Pages (from-to)428-431
Number of pages4
JournalCornea
Volume33
Issue number4
DOIs
StatePublished - Jan 1 2014

Fingerprint

Descemet Membrane
Corneal Transplantation
Optical Coherence Tomography
Tissue Donors
Emphysema
Air
Biophysics
Injections
Anterior Chamber
Artifacts
Cornea
Technology
Cannula

Keywords

  • Descemet membrane endothelial keratoplasty
  • donor tissue preparation
  • intraoperative real-time imaging
  • spectral domain optical coherence tomography

ASJC Scopus subject areas

  • Ophthalmology

Cite this

High-resolution optical coherence tomography-guided donor tissue preparation for descemet membrane endothelial keratoplasty using the reverse big bubble technique. / Cabot, Florence; Kankariya, Vardhaman P.; Ruggeri, Marco; Yoo, Sonia H; Vaddavalli, Pravin K.; Parel, Jean-Marie A; Kymionis, George D.

In: Cornea, Vol. 33, No. 4, 01.01.2014, p. 428-431.

Research output: Contribution to journalArticle

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