TY - JOUR
T1 - High-resolution, noninvasive longitudinal live imaging of immune responses
AU - Abdulreda, Midhat H.
AU - Faleo, Gaetano
AU - Molano, Ruth Damaris
AU - Lopez-Cabezas, Maite
AU - Molina, Judith
AU - Tan, Yaohong
AU - Ron Echeverria, Oscar A.
AU - Zahr-Akrawi, Elsie
AU - Rodriguez-Diaz, Rayner
AU - Edlund, Patrick K.
AU - Leibiger, Ingo
AU - Bayer, Allison L.
AU - Perez, Victor
AU - Ricordi, Camillo
AU - Caicedo, Alejandro
AU - Pileggi, Antonello
AU - Berggren, Per Olof
PY - 2011/8/2
Y1 - 2011/8/2
N2 - Intravital imaging emerged as an indispensible tool in biological research, and a variety of imaging techniques have been developed to noninvasively monitor tissues in vivo. However, most of the current techniques lack the resolution to study events at the single-cell level. Although intravital multiphoton microscopy has addressed this limitation, the need for repeated noninvasive access to the same tissue in longitudinal in vivo studies remains largely unmet. We now report on a previously unexplored approach to study immune responses after transplantation of pancreatic islets into the anterior chamber of the mouse eye. This approach enabled (i) longitudinal, noninvasive imaging of transplanted tissues in vivo; (ii) in vivo cytolabeling to assess cellular phenotype and viability in situ; (iii) local intervention by topical application or intraocular injection; and (iv) real-time tracking of infiltrating immune cells in the target tissue.
AB - Intravital imaging emerged as an indispensible tool in biological research, and a variety of imaging techniques have been developed to noninvasively monitor tissues in vivo. However, most of the current techniques lack the resolution to study events at the single-cell level. Although intravital multiphoton microscopy has addressed this limitation, the need for repeated noninvasive access to the same tissue in longitudinal in vivo studies remains largely unmet. We now report on a previously unexplored approach to study immune responses after transplantation of pancreatic islets into the anterior chamber of the mouse eye. This approach enabled (i) longitudinal, noninvasive imaging of transplanted tissues in vivo; (ii) in vivo cytolabeling to assess cellular phenotype and viability in situ; (iii) local intervention by topical application or intraocular injection; and (iv) real-time tracking of infiltrating immune cells in the target tissue.
KW - Allorejection
KW - In vivo imaging
KW - Infiltration
KW - Islet grafts
KW - T-cell dynamics
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U2 - 10.1073/pnas.1105002108
DO - 10.1073/pnas.1105002108
M3 - Article
C2 - 21768391
AN - SCOPUS:79961237839
VL - 108
SP - 12863
EP - 12868
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 31
ER -