High-resolution, noninvasive longitudinal live imaging of immune responses

Midhat H. Abdulreda, Gaetano Faleo, Ruth Damaris Molano, Maite Lopez-Cabezas, Judith Molina, Yaohong Tan, Oscar A. Ron Echeverria, Elsie Zahr-Akrawi, Rayner Rodriguez-Diaz, Patrick K. Edlund, Ingo Leibiger, Allison L. Bayer, Victor Perez, Camillo Ricordi, Alejandro Caicedo, Antonello Pileggi, Per Olof Berggren

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

Intravital imaging emerged as an indispensible tool in biological research, and a variety of imaging techniques have been developed to noninvasively monitor tissues in vivo. However, most of the current techniques lack the resolution to study events at the single-cell level. Although intravital multiphoton microscopy has addressed this limitation, the need for repeated noninvasive access to the same tissue in longitudinal in vivo studies remains largely unmet. We now report on a previously unexplored approach to study immune responses after transplantation of pancreatic islets into the anterior chamber of the mouse eye. This approach enabled (i) longitudinal, noninvasive imaging of transplanted tissues in vivo; (ii) in vivo cytolabeling to assess cellular phenotype and viability in situ; (iii) local intervention by topical application or intraocular injection; and (iv) real-time tracking of infiltrating immune cells in the target tissue.

Original languageEnglish (US)
Pages (from-to)12863-12868
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume108
Issue number31
DOIs
StatePublished - Aug 2 2011

Keywords

  • Allorejection
  • In vivo imaging
  • Infiltration
  • Islet grafts
  • T-cell dynamics

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'High-resolution, noninvasive longitudinal live imaging of immune responses'. Together they form a unique fingerprint.

  • Cite this

    Abdulreda, M. H., Faleo, G., Molano, R. D., Lopez-Cabezas, M., Molina, J., Tan, Y., Ron Echeverria, O. A., Zahr-Akrawi, E., Rodriguez-Diaz, R., Edlund, P. K., Leibiger, I., Bayer, A. L., Perez, V., Ricordi, C., Caicedo, A., Pileggi, A., & Berggren, P. O. (2011). High-resolution, noninvasive longitudinal live imaging of immune responses. Proceedings of the National Academy of Sciences of the United States of America, 108(31), 12863-12868. https://doi.org/10.1073/pnas.1105002108