High-resolution, noninvasive longitudinal live imaging of immune responses

Midhat H Abdulreda, Gaetano Faleo, Ruth Molano, Maite Lopez-Cabezas, Judith Molina, Yaohong Tan, Oscar A. Ron Echeverria, Elsie Zahr-Akrawi, Rayner Rodriguez Diaz, Patrick K. Edlund, Ingo Leibiger, Allison L Bayer, Victor L Perez Quinones, Camillo Ricordi, Diego A Caicedo-Vierkant, Antonello Pileggi, Per Olof Berggren

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Intravital imaging emerged as an indispensible tool in biological research, and a variety of imaging techniques have been developed to noninvasively monitor tissues in vivo. However, most of the current techniques lack the resolution to study events at the single-cell level. Although intravital multiphoton microscopy has addressed this limitation, the need for repeated noninvasive access to the same tissue in longitudinal in vivo studies remains largely unmet. We now report on a previously unexplored approach to study immune responses after transplantation of pancreatic islets into the anterior chamber of the mouse eye. This approach enabled (i) longitudinal, noninvasive imaging of transplanted tissues in vivo; (ii) in vivo cytolabeling to assess cellular phenotype and viability in situ; (iii) local intervention by topical application or intraocular injection; and (iv) real-time tracking of infiltrating immune cells in the target tissue.

Original languageEnglish
Pages (from-to)12863-12868
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume108
Issue number31
DOIs
StatePublished - Aug 2 2011

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Intraocular Injections
Islets of Langerhans Transplantation
Anterior Chamber
Phenotype
Research
Intravital Microscopy

Keywords

  • Allorejection
  • In vivo imaging
  • Infiltration
  • Islet grafts
  • T-cell dynamics

ASJC Scopus subject areas

  • General

Cite this

High-resolution, noninvasive longitudinal live imaging of immune responses. / Abdulreda, Midhat H; Faleo, Gaetano; Molano, Ruth; Lopez-Cabezas, Maite; Molina, Judith; Tan, Yaohong; Ron Echeverria, Oscar A.; Zahr-Akrawi, Elsie; Rodriguez Diaz, Rayner; Edlund, Patrick K.; Leibiger, Ingo; Bayer, Allison L; Perez Quinones, Victor L; Ricordi, Camillo; Caicedo-Vierkant, Diego A; Pileggi, Antonello; Berggren, Per Olof.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, No. 31, 02.08.2011, p. 12863-12868.

Research output: Contribution to journalArticle

Abdulreda, MH, Faleo, G, Molano, R, Lopez-Cabezas, M, Molina, J, Tan, Y, Ron Echeverria, OA, Zahr-Akrawi, E, Rodriguez Diaz, R, Edlund, PK, Leibiger, I, Bayer, AL, Perez Quinones, VL, Ricordi, C, Caicedo-Vierkant, DA, Pileggi, A & Berggren, PO 2011, 'High-resolution, noninvasive longitudinal live imaging of immune responses', Proceedings of the National Academy of Sciences of the United States of America, vol. 108, no. 31, pp. 12863-12868. https://doi.org/10.1073/pnas.1105002108
Abdulreda, Midhat H ; Faleo, Gaetano ; Molano, Ruth ; Lopez-Cabezas, Maite ; Molina, Judith ; Tan, Yaohong ; Ron Echeverria, Oscar A. ; Zahr-Akrawi, Elsie ; Rodriguez Diaz, Rayner ; Edlund, Patrick K. ; Leibiger, Ingo ; Bayer, Allison L ; Perez Quinones, Victor L ; Ricordi, Camillo ; Caicedo-Vierkant, Diego A ; Pileggi, Antonello ; Berggren, Per Olof. / High-resolution, noninvasive longitudinal live imaging of immune responses. In: Proceedings of the National Academy of Sciences of the United States of America. 2011 ; Vol. 108, No. 31. pp. 12863-12868.
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