High-resolution deletion mapping of chromosome 14 in stromal tumors of the gastrointestinal tract suggests two distinct tumor suppressor loci

Wa'el El-Rifai, Maarit Sarlomo-Rikala, Leif C. Andersson, Markku Miettinen, Sakari Knuutila

Research output: Contribution to journalArticlepeer-review

74 Scopus citations

Abstract

DNA copy number losses at chromosome arm 14q are the most frequently occurring aberrations in gastrointestinal stromal tumors (GISTs). To characterize the deletion at 14q, we performed comparative genomic hybridization (CGH) and high-resolution deletion mapping using a panel of 32 polymorphic microsatellite markers in 30 GISTs. The GISTs were classified according to their metastatic potential and mitotic counts into 15 low-risk and 15 high-risk tumors. Losses with a minimal common overlapping region at 14q12-q24 were detected by CGH in 16 tumors (53) (nine low-risk and seven high-risk). Investigation with microsatellite markers was informative in 690 analyses (72%). Loss of heterozygosity (LOH) with at least one marker was detected in 279 analyses in 24 tumors (80%). Deletions were equally frequent in low-risk and high-risk GISTs. Two common deletion regions were identified at 14q11.1-q12 and 14q23-q24.3. The highest frequencies of deletions were seen in regions corresponding to markers D14S283 (20/28, 71%) at 14q11.1-q12 and D14S258 (17/27, 63%) at 14q23-q24, suggesting that these are two tumor suppressor loci. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)387-391
Number of pages5
JournalGenes Chromosomes and Cancer
Volume27
Issue number4
DOIs
StatePublished - Apr 1 2000
Externally publishedYes

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

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