High rates of HCO3- secretion and Cl- Absorption against adverse gradients in the marine teleost intestine: The involvement of an electrogenic anion exchanger and H+-pump metabolon?

Martin Grosell, E. M. Mager, C. Williams, J. R. Taylor

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Anion exchange contributes significantly to intestinal Cl- absorption in marine teleost fish and is thus vital for successful osmoregulation. This anion exchange process leads to high luminal HCO3- concentrations (up to ∼100mmoll-1) and high pH and results in the formation of CaCO3 precipitates in the intestinal lumen. Recent advances in our understanding of the transport processes involved in intestinal anion exchange in marine teleost fish include the demonstration of a role for the H+-pump (V- ATPase) in apical H+ extrusion and the presence of an electrogenic (nHCO 3-/Cl -) exchange protein (SLC26a6). The H+-V-ATPase defends against cellular acidification, which might otherwise occur as a consequence of the high rates of base secretion. In addition, apical H+ extrusion probably maintains lower HCO3- concentrations in the unstirred layer at the apical surface than in the bulk luminal fluids and thus facilitates continued anion exchange. Furthermore, H+-V-ATPase activity hyperpolarizes the apical membrane potential that provides the driving force for apical electrogenic nHCO3-/Cl- exchange, which appears to occur against both Cl- and HCO3- electrochemical gradients. We propose that a similar coupling between apical H+ extrusion and nHCO3 -/Cl-. xchange accounts for Cl- uptake in freshwater fish and amphibians against very steep Cl- gradients.

Original languageEnglish
Pages (from-to)1684-1696
Number of pages13
JournalJournal of Experimental Biology
Volume212
Issue number11
DOIs
StatePublished - Jun 1 2009

Fingerprint

Proton Pumps
antiporters
proton pump
anion exchange
teleost
secretion
H-transporting ATP synthase
Intestines
Anions
anion
ion exchange
pump
intestines
extrusion
Fishes
Proton-Translocating ATPases
marine fish
fish
Osmoregulation
osmoregulation

Keywords

  • Fish
  • Osmoregulation
  • PH-stat titration
  • Seawater
  • Water absorption

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Physiology
  • Insect Science
  • Aquatic Science

Cite this

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abstract = "Anion exchange contributes significantly to intestinal Cl- absorption in marine teleost fish and is thus vital for successful osmoregulation. This anion exchange process leads to high luminal HCO3- concentrations (up to ∼100mmoll-1) and high pH and results in the formation of CaCO3 precipitates in the intestinal lumen. Recent advances in our understanding of the transport processes involved in intestinal anion exchange in marine teleost fish include the demonstration of a role for the H+-pump (V- ATPase) in apical H+ extrusion and the presence of an electrogenic (nHCO 3-/Cl -) exchange protein (SLC26a6). The H+-V-ATPase defends against cellular acidification, which might otherwise occur as a consequence of the high rates of base secretion. In addition, apical H+ extrusion probably maintains lower HCO3- concentrations in the unstirred layer at the apical surface than in the bulk luminal fluids and thus facilitates continued anion exchange. Furthermore, H+-V-ATPase activity hyperpolarizes the apical membrane potential that provides the driving force for apical electrogenic nHCO3-/Cl- exchange, which appears to occur against both Cl- and HCO3- electrochemical gradients. We propose that a similar coupling between apical H+ extrusion and nHCO3 -/Cl-. xchange accounts for Cl- uptake in freshwater fish and amphibians against very steep Cl- gradients.",
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AU - Taylor, J. R.

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