High performance liquid chromatography determination of ethylene glycol and ethylene chlorohydrin in tissues

N. M. Wu, Theodore Malinin

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A simultaneous determination of ethylene glycol (EG) and ethylene chlorohydrin (EC), which are formed during sterilization of bone and dura mater with ethylene oxide, has been reported. The EG and EC residues in tissues were extracted with HPLC grade water and analyzed by reversed-phase high-performance liquid chromatography (HPLC) with a RefractoMonitor detector. The isocratic elution was employed with HPLC grade water as the solvent. Since the reference chamber of the detector was filled with solvent and was not changed during the run, the use of gradient programming was not employed. With spherisorb ODS (5 μm) C18 column and 20 μL sample injection, the retention times for EG and EC were (104 ± 2) sec and (202 ± 4) sec, respectively. The unknown concentration was calculated from the calibration curve and the weight of the sample tissue. The HPLC analysis provided a simple, accurate and reproducible method of determination of EG and EC residues in ethylene-oxide sterilized tissues.

Original languageEnglish
Pages (from-to)63-66
Number of pages4
JournalJournal of Analytical Toxicology
Volume11
Issue number2
StatePublished - Jan 1 1987

Fingerprint

Ethylene Chlorohydrin
Ethylene Glycol
High performance liquid chromatography
Ethylene glycol
ethylene
liquid chromatography
Ethylene
High Pressure Liquid Chromatography
Tissue
Ethylene Oxide
Detectors
Dura Mater
Water
Reverse-Phase Chromatography
Calibration
Bone
Oxides
Weights and Measures
Bone and Bones
Injections

ASJC Scopus subject areas

  • Analytical Chemistry
  • Health, Toxicology and Mutagenesis
  • Toxicology

Cite this

High performance liquid chromatography determination of ethylene glycol and ethylene chlorohydrin in tissues. / Wu, N. M.; Malinin, Theodore.

In: Journal of Analytical Toxicology, Vol. 11, No. 2, 01.01.1987, p. 63-66.

Research output: Contribution to journalArticle

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