High-performance chromatographic separation of cerebrosides

Renaud Sicard, Ralf Landgraf

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

High-performance thin-layer chromatography (HPTLC) is a very robust, fast, and inexpensive technique that enables separation of complex mixtures. Here, we describe the analytical separation of glucosylceramide and galactosylceramide by HPTLC. This technique can be used for quantitation purposes but also with small modification for subsequent mass spectrum analyses for structural determination.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages57-63
Number of pages7
Volume1609
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1609
ISSN (Print)1064-3745

Fingerprint

Cerebrosides
Thin Layer Chromatography
Galactosylceramides
Glucosylceramides
Complex Mixtures
Mass Spectrometry

Keywords

  • Cerebrosides
  • Galactosylceramide
  • Glucosylceramide
  • High-performance thin-layer chromatography
  • Orcinol reagent

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Sicard, R., & Landgraf, R. (2017). High-performance chromatographic separation of cerebrosides. In Methods in Molecular Biology (Vol. 1609, pp. 57-63). (Methods in Molecular Biology; Vol. 1609). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6996-8_7

High-performance chromatographic separation of cerebrosides. / Sicard, Renaud; Landgraf, Ralf.

Methods in Molecular Biology. Vol. 1609 Humana Press Inc., 2017. p. 57-63 (Methods in Molecular Biology; Vol. 1609).

Research output: Chapter in Book/Report/Conference proceedingChapter

Sicard, R & Landgraf, R 2017, High-performance chromatographic separation of cerebrosides. in Methods in Molecular Biology. vol. 1609, Methods in Molecular Biology, vol. 1609, Humana Press Inc., pp. 57-63. https://doi.org/10.1007/978-1-4939-6996-8_7
Sicard R, Landgraf R. High-performance chromatographic separation of cerebrosides. In Methods in Molecular Biology. Vol. 1609. Humana Press Inc. 2017. p. 57-63. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6996-8_7
Sicard, Renaud ; Landgraf, Ralf. / High-performance chromatographic separation of cerebrosides. Methods in Molecular Biology. Vol. 1609 Humana Press Inc., 2017. pp. 57-63 (Methods in Molecular Biology).
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