Vysokomolekuliarnye formy GTP-sviazyvaiushchikh reguliatornykh belkov iz mozzhechka byka.

Translated title of the contribution: High molecular weight forms of GTP-binding regulatory proteins from the bovine cerebellum

V. L. Voeǐkov, A. N. Pronin, Vladlen Z Slepak, A. N. Chestukhin, A. B. Shlenskiǐ

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A new form of a low Km GTPase belonging to the family of regulatory GTP-binding G-proteins has been identified in bovine cerebellum. The molecular weight of this G-protein is several times as high as that of other G-proteins known to be alpha beta gamma heterotrimers: i. e., Gs, Gi, Go, transducin and a new G-protein which had recently been isolated in our laboratory from bovine cerebellum. The high molecular weight G-protein is stable against dissociation; its molecular mass does not change after treatment with DTT, colchicine and NaF. Using antibodies against the alpha-subunit of the formerly isolated cerebellar G-protein and the transducin beta-subunit, it was demonstrated that the both immunoreactive subunits are present in the high molecular weight G-protein. The two forms of the cerebellar G-proteins, i. e., "high" and "low molecular weight" ones, differ drastically in terms of the Mg2+ effect on their GTPase activity. Whereas at submicromolar concentrations of Mg2+ the GTPase activity of the former is virtually absent, the GTPase activity of the latter is more elevated in the presence of EDTA than in the presence of Mg2+.

Original languageUndefined/Unknown
Pages (from-to)1766-1769
Number of pages4
JournalBiokhimiya
Volume52
Issue number11
StatePublished - Nov 1 1987
Externally publishedYes

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Guanosine Triphosphate
GTP-Binding Proteins
Molecular weight
GTP Phosphohydrolases
Proteins
Transducin
Colchicine
Molecular mass
Edetic Acid
Antibodies

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Voeǐkov, V. L., Pronin, A. N., Slepak, V. Z., Chestukhin, A. N., & Shlenskiǐ, A. B. (1987). Vysokomolekuliarnye formy GTP-sviazyvaiushchikh reguliatornykh belkov iz mozzhechka byka. Biokhimiya, 52(11), 1766-1769.

Vysokomolekuliarnye formy GTP-sviazyvaiushchikh reguliatornykh belkov iz mozzhechka byka. / Voeǐkov, V. L.; Pronin, A. N.; Slepak, Vladlen Z; Chestukhin, A. N.; Shlenskiǐ, A. B.

In: Biokhimiya, Vol. 52, No. 11, 01.11.1987, p. 1766-1769.

Research output: Contribution to journalArticle

Voeǐkov, VL, Pronin, AN, Slepak, VZ, Chestukhin, AN & Shlenskiǐ, AB 1987, 'Vysokomolekuliarnye formy GTP-sviazyvaiushchikh reguliatornykh belkov iz mozzhechka byka.', Biokhimiya, vol. 52, no. 11, pp. 1766-1769.
Voeǐkov VL, Pronin AN, Slepak VZ, Chestukhin AN, Shlenskiǐ AB. Vysokomolekuliarnye formy GTP-sviazyvaiushchikh reguliatornykh belkov iz mozzhechka byka. Biokhimiya. 1987 Nov 1;52(11):1766-1769.
Voeǐkov, V. L. ; Pronin, A. N. ; Slepak, Vladlen Z ; Chestukhin, A. N. ; Shlenskiǐ, A. B. / Vysokomolekuliarnye formy GTP-sviazyvaiushchikh reguliatornykh belkov iz mozzhechka byka. In: Biokhimiya. 1987 ; Vol. 52, No. 11. pp. 1766-1769.
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AB - A new form of a low Km GTPase belonging to the family of regulatory GTP-binding G-proteins has been identified in bovine cerebellum. The molecular weight of this G-protein is several times as high as that of other G-proteins known to be alpha beta gamma heterotrimers: i. e., Gs, Gi, Go, transducin and a new G-protein which had recently been isolated in our laboratory from bovine cerebellum. The high molecular weight G-protein is stable against dissociation; its molecular mass does not change after treatment with DTT, colchicine and NaF. Using antibodies against the alpha-subunit of the formerly isolated cerebellar G-protein and the transducin beta-subunit, it was demonstrated that the both immunoreactive subunits are present in the high molecular weight G-protein. The two forms of the cerebellar G-proteins, i. e., "high" and "low molecular weight" ones, differ drastically in terms of the Mg2+ effect on their GTPase activity. Whereas at submicromolar concentrations of Mg2+ the GTPase activity of the former is virtually absent, the GTPase activity of the latter is more elevated in the presence of EDTA than in the presence of Mg2+.

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