High-fat diets suppress CD3 and CD25 expression on the surface of murine lymphocytes

Michael D. Peck; Frederick L. Moffat; Paul B. Spalding; Tieran Han; Wenche Jy

doi:10.1016/S0899-9007(99)00300-7

High-fat diets suppress CD3 and CD25 expression on the surface of murine lymphocytes

Michael D. Peck, Frederick L. Moffat, Paul B. Spalding, Tieran Han, Wenche Jy

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

To determine the effects of dietary fats on surface antigen expression, we tested the effects of amount and type of dietary fat on murine lymphocytes. Mice were fed diets with 12 en%, 23 en%, or 47 en% fat containing coconut, olive, safflower, or linseed oil. After 2 wk of ad libitum feeding, the mice were killed and splenic lymphocytes were harvested. Lymphocytes were incubated with fluorescent-tagged monoclonal antibodies and assayed for mean and total surface expression using flow cytometry. Our results show that high-fat (47 en%) diets suppress expression of CD3 and CD25 antigens. We also found that linseed-oil diets suppress expression of CD11a but enhance expression of CD25 antigens. Both CD3 and CD25 are critical for lymphocyte activation, and we conclude that immunosuppression associated with high-fat diets may be associated with suppression of these surface antigens. Copyright (C) 2000 Elsevier Science Inc.

Original language	English (US)
Pages (from-to)	278-283
Number of pages	6
Journal	Nutrition
Volume	16
Issue number	4
DOIs	https://doi.org/10.1016/S0899-9007(99)00300-7
State	Published - Apr 2000

Keywords

CD11a
CD18
CD25
CD3
Dietary lipids
Interleukin-2 receptor
T lymphocytes

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Surgery
Medicine (miscellaneous)

UN SDGs

This output contributes to the following Sustainable Development Goal(s)

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10.1016/S0899-9007(99)00300-7

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title = "High-fat diets suppress CD3 and CD25 expression on the surface of murine lymphocytes",

abstract = "To determine the effects of dietary fats on surface antigen expression, we tested the effects of amount and type of dietary fat on murine lymphocytes. Mice were fed diets with 12 en%, 23 en%, or 47 en% fat containing coconut, olive, safflower, or linseed oil. After 2 wk of ad libitum feeding, the mice were killed and splenic lymphocytes were harvested. Lymphocytes were incubated with fluorescent-tagged monoclonal antibodies and assayed for mean and total surface expression using flow cytometry. Our results show that high-fat (47 en%) diets suppress expression of CD3 and CD25 antigens. We also found that linseed-oil diets suppress expression of CD11a but enhance expression of CD25 antigens. Both CD3 and CD25 are critical for lymphocyte activation, and we conclude that immunosuppression associated with high-fat diets may be associated with suppression of these surface antigens. Copyright (C) 2000 Elsevier Science Inc.",

keywords = "CD11a, CD18, CD25, CD3, Dietary lipids, Interleukin-2 receptor, T lymphocytes",

author = "Peck, {Michael D.} and Moffat, {Frederick L.} and Spalding, {Paul B.} and Tieran Han and Wenche Jy",

note = "Funding Information: This work was supported by the NRI Competitive Grants Program/USDA 9400546 and by the International Olive Oil Council. The authors acknowledge the contributions to protocol design and methodology made by George F. Babcock, Ph.D., Department of Surgery, University of Cincinnati School of Medicine, and by Ron Rose, Ph.D., Research Nutritionist at Harlan Teklad. The authors also appreciate the editing skills of Carol Winkelman, M.A. ",

year = "2000",

month = apr,

doi = "10.1016/S0899-9007(99)00300-7",

language = "English (US)",

volume = "16",

pages = "278--283",

journal = "Nutrition",

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TY - JOUR

T1 - High-fat diets suppress CD3 and CD25 expression on the surface of murine lymphocytes

AU - Peck, Michael D.

AU - Moffat, Frederick L.

AU - Spalding, Paul B.

AU - Han, Tieran

AU - Jy, Wenche

N1 - Funding Information: This work was supported by the NRI Competitive Grants Program/USDA 9400546 and by the International Olive Oil Council. The authors acknowledge the contributions to protocol design and methodology made by George F. Babcock, Ph.D., Department of Surgery, University of Cincinnati School of Medicine, and by Ron Rose, Ph.D., Research Nutritionist at Harlan Teklad. The authors also appreciate the editing skills of Carol Winkelman, M.A.

PY - 2000/4

Y1 - 2000/4

N2 - To determine the effects of dietary fats on surface antigen expression, we tested the effects of amount and type of dietary fat on murine lymphocytes. Mice were fed diets with 12 en%, 23 en%, or 47 en% fat containing coconut, olive, safflower, or linseed oil. After 2 wk of ad libitum feeding, the mice were killed and splenic lymphocytes were harvested. Lymphocytes were incubated with fluorescent-tagged monoclonal antibodies and assayed for mean and total surface expression using flow cytometry. Our results show that high-fat (47 en%) diets suppress expression of CD3 and CD25 antigens. We also found that linseed-oil diets suppress expression of CD11a but enhance expression of CD25 antigens. Both CD3 and CD25 are critical for lymphocyte activation, and we conclude that immunosuppression associated with high-fat diets may be associated with suppression of these surface antigens. Copyright (C) 2000 Elsevier Science Inc.

AB - To determine the effects of dietary fats on surface antigen expression, we tested the effects of amount and type of dietary fat on murine lymphocytes. Mice were fed diets with 12 en%, 23 en%, or 47 en% fat containing coconut, olive, safflower, or linseed oil. After 2 wk of ad libitum feeding, the mice were killed and splenic lymphocytes were harvested. Lymphocytes were incubated with fluorescent-tagged monoclonal antibodies and assayed for mean and total surface expression using flow cytometry. Our results show that high-fat (47 en%) diets suppress expression of CD3 and CD25 antigens. We also found that linseed-oil diets suppress expression of CD11a but enhance expression of CD25 antigens. Both CD3 and CD25 are critical for lymphocyte activation, and we conclude that immunosuppression associated with high-fat diets may be associated with suppression of these surface antigens. Copyright (C) 2000 Elsevier Science Inc.

KW - CD11a

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KW - CD25

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KW - Dietary lipids

KW - Interleukin-2 receptor

KW - T lymphocytes

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High-fat diets suppress CD3 and CD25 expression on the surface of murine lymphocytes

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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