Abstract
To determine the effects of dietary fats on surface antigen expression, we tested the effects of amount and type of dietary fat on murine lymphocytes. Mice were fed diets with 12 en%, 23 en%, or 47 en% fat containing coconut, olive, safflower, or linseed oil. After 2 wk of ad libitum feeding, the mice were killed and splenic lymphocytes were harvested. Lymphocytes were incubated with fluorescent-tagged monoclonal antibodies and assayed for mean and total surface expression using flow cytometry. Our results show that high-fat (47 en%) diets suppress expression of CD3 and CD25 antigens. We also found that linseed-oil diets suppress expression of CD11a but enhance expression of CD25 antigens. Both CD3 and CD25 are critical for lymphocyte activation, and we conclude that immunosuppression associated with high-fat diets may be associated with suppression of these surface antigens. Copyright (C) 2000 Elsevier Science Inc.
| Original language | English |
|---|---|
| Pages (from-to) | 278-283 |
| Number of pages | 6 |
| Journal | Nutrition |
| Volume | 16 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 1 2000 |
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Keywords
- CD11a
- CD18
- CD25
- CD3
- Dietary lipids
- Interleukin-2 receptor
- T lymphocytes
ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism
- Surgery
- Medicine (miscellaneous)
Cite this
High-fat diets suppress CD3 and CD25 expression on the surface of murine lymphocytes. / Peck, Michael D.; Moffat, Frederick L; Spalding, Paul B.; Han, Tieran; Jy, Wenche.
In: Nutrition, Vol. 16, No. 4, 01.04.2000, p. 278-283.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - High-fat diets suppress CD3 and CD25 expression on the surface of murine lymphocytes
AU - Peck, Michael D.
AU - Moffat, Frederick L
AU - Spalding, Paul B.
AU - Han, Tieran
AU - Jy, Wenche
PY - 2000/4/1
Y1 - 2000/4/1
N2 - To determine the effects of dietary fats on surface antigen expression, we tested the effects of amount and type of dietary fat on murine lymphocytes. Mice were fed diets with 12 en%, 23 en%, or 47 en% fat containing coconut, olive, safflower, or linseed oil. After 2 wk of ad libitum feeding, the mice were killed and splenic lymphocytes were harvested. Lymphocytes were incubated with fluorescent-tagged monoclonal antibodies and assayed for mean and total surface expression using flow cytometry. Our results show that high-fat (47 en%) diets suppress expression of CD3 and CD25 antigens. We also found that linseed-oil diets suppress expression of CD11a but enhance expression of CD25 antigens. Both CD3 and CD25 are critical for lymphocyte activation, and we conclude that immunosuppression associated with high-fat diets may be associated with suppression of these surface antigens. Copyright (C) 2000 Elsevier Science Inc.
AB - To determine the effects of dietary fats on surface antigen expression, we tested the effects of amount and type of dietary fat on murine lymphocytes. Mice were fed diets with 12 en%, 23 en%, or 47 en% fat containing coconut, olive, safflower, or linseed oil. After 2 wk of ad libitum feeding, the mice were killed and splenic lymphocytes were harvested. Lymphocytes were incubated with fluorescent-tagged monoclonal antibodies and assayed for mean and total surface expression using flow cytometry. Our results show that high-fat (47 en%) diets suppress expression of CD3 and CD25 antigens. We also found that linseed-oil diets suppress expression of CD11a but enhance expression of CD25 antigens. Both CD3 and CD25 are critical for lymphocyte activation, and we conclude that immunosuppression associated with high-fat diets may be associated with suppression of these surface antigens. Copyright (C) 2000 Elsevier Science Inc.
KW - CD11a
KW - CD18
KW - CD25
KW - CD3
KW - Dietary lipids
KW - Interleukin-2 receptor
KW - T lymphocytes
UR - http://www.scopus.com/inward/record.url?scp=0034025035&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034025035&partnerID=8YFLogxK
U2 - 10.1016/S0899-9007(99)00300-7
DO - 10.1016/S0899-9007(99)00300-7
M3 - Article
C2 - 10758364
AN - SCOPUS:0034025035
VL - 16
SP - 278
EP - 283
JO - Nutrition
JF - Nutrition
SN - 0899-9007
IS - 4
ER -