High-density collagen gel tubes as a matrix for primary human bladder smooth muscle cells

Lionel A. Micol, Michael Ananta, Eva Maria Engelhardt, Vivek C. Mudera, Robert A. Brown, Jeffrey A. Hubbell, Peter Frey

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Tissue-engineered grafts for the urinary tract are being investigated for the potential treatment of several urologic diseases. These grafts, predominantly tubular-shaped, usually require in vitro culture prior to implantation to allow cell engraftment on initially cell-free scaffolds. We have developed a method to produce tubular-shaped collagen scaffolds based on plastic compression. Our approach produces a ready cell-seeded graft that does not need further in vitro culture prior to implantation. The tubular collagen scaffolds were in particular investigated for their structural, mechanical and biological properties. The resulting construct showed an especially high collagen density, and was characterized by favorable mechanical properties assessed by axial extension and radial dilation. Young modulus in particular was greater than non-compressed collagen tubes. Seeding densities affected proliferation rate of primary human bladder smooth muscle cells. An optimal seeding density of 106 cells per construct resulted in a 25-fold increase in Alamar blue-based fluorescence after 2 wk in culture. These high-density collagen gel tubes, ready seeded with smooth muscle cells could be further seeded with urothelial cells, drastically shortening the production time of graft for urinary tract regeneration.

Original languageEnglish
Pages (from-to)1543-1548
Number of pages6
JournalBiomaterials
Volume32
Issue number6
DOIs
StatePublished - Feb 1 2011
Externally publishedYes

Fingerprint

Collagen
Smooth Muscle Myocytes
Muscle
Urinary Bladder
Gels
Grafts
Cells
Scaffolds
Transplants
Scaffolds (biology)
Urinary Tract
Urologic Diseases
Elastic Modulus
Plastics
Regeneration
Dilatation
Compaction
Cell Count
Elastic moduli
Fluorescence

Keywords

  • Bladder tissue engineering
  • Cell proliferation
  • Collagen
  • Mechanical properties
  • Smooth muscle cell
  • Urinary tract

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Micol, L. A., Ananta, M., Engelhardt, E. M., Mudera, V. C., Brown, R. A., Hubbell, J. A., & Frey, P. (2011). High-density collagen gel tubes as a matrix for primary human bladder smooth muscle cells. Biomaterials, 32(6), 1543-1548. https://doi.org/10.1016/j.biomaterials.2010.10.028

High-density collagen gel tubes as a matrix for primary human bladder smooth muscle cells. / Micol, Lionel A.; Ananta, Michael; Engelhardt, Eva Maria; Mudera, Vivek C.; Brown, Robert A.; Hubbell, Jeffrey A.; Frey, Peter.

In: Biomaterials, Vol. 32, No. 6, 01.02.2011, p. 1543-1548.

Research output: Contribution to journalArticle

Micol, LA, Ananta, M, Engelhardt, EM, Mudera, VC, Brown, RA, Hubbell, JA & Frey, P 2011, 'High-density collagen gel tubes as a matrix for primary human bladder smooth muscle cells', Biomaterials, vol. 32, no. 6, pp. 1543-1548. https://doi.org/10.1016/j.biomaterials.2010.10.028
Micol LA, Ananta M, Engelhardt EM, Mudera VC, Brown RA, Hubbell JA et al. High-density collagen gel tubes as a matrix for primary human bladder smooth muscle cells. Biomaterials. 2011 Feb 1;32(6):1543-1548. https://doi.org/10.1016/j.biomaterials.2010.10.028
Micol, Lionel A. ; Ananta, Michael ; Engelhardt, Eva Maria ; Mudera, Vivek C. ; Brown, Robert A. ; Hubbell, Jeffrey A. ; Frey, Peter. / High-density collagen gel tubes as a matrix for primary human bladder smooth muscle cells. In: Biomaterials. 2011 ; Vol. 32, No. 6. pp. 1543-1548.
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