High-affinity binding site for a specific nuclear protein in the human IgM gene

L. Hennighausen, U. Siebenlist, D. Danner, P. Leder, D. Rawlins, Philip J Rosenfeld, T. Kelly

Research output: Contribution to journalArticle

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Abstract

Proteins binding to specific regions of DNA with high affinity frequently govern or regulate reactions at the gene level. We have identified a high-affinity binding site in the immunoglobulin μ gene that binds a specific nuclear protein, and have now characterized it fully using nuclear factor 1 (NF-1), a protein purified from the nuclei of HeLa cells and required for the in vitro replication of adenovirus (Ad) DNA. NF-1 protects a 25-base pair (bp) double-stranded segment of DNA which shares a consensus sequence, 5' TGGA/CNNNNNGCCAA 3', with similar binding sites in the Ad-5' terminal repeat and the human c-myc gene. Although this site differs from the enhancer region, a biological function is suggested by the fact that it is DNase I hypersensitive in immunoglobulin-producing lymphoblastoid cells. The binding site for the NF-1 protein in the μ gene, by analogy with the site in the Ad-5 terminal repeat, may represent one component of a cellular origin of replication; alternatively, it may be responsible for the activation of the chromatin in this region.

Original languageEnglish
Pages (from-to)289-292
Number of pages4
JournalNature
Volume314
Issue number6008
DOIs
StatePublished - Jul 17 1985
Externally publishedYes

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NFI Transcription Factors
Nuclear Proteins
Adenoviridae
Immunoglobulin M
Terminal Repeat Sequences
Binding Sites
Genes
Antibody-Producing Cells
Immunoglobulin Genes
myc Genes
Replication Origin
Deoxyribonuclease I
DNA
Consensus Sequence
DNA Replication
HeLa Cells
Protein Binding
Base Pairing
Chromatin
Proteins

ASJC Scopus subject areas

  • General

Cite this

Hennighausen, L., Siebenlist, U., Danner, D., Leder, P., Rawlins, D., Rosenfeld, P. J., & Kelly, T. (1985). High-affinity binding site for a specific nuclear protein in the human IgM gene. Nature, 314(6008), 289-292. https://doi.org/10.1038/314289a0

High-affinity binding site for a specific nuclear protein in the human IgM gene. / Hennighausen, L.; Siebenlist, U.; Danner, D.; Leder, P.; Rawlins, D.; Rosenfeld, Philip J; Kelly, T.

In: Nature, Vol. 314, No. 6008, 17.07.1985, p. 289-292.

Research output: Contribution to journalArticle

Hennighausen, L, Siebenlist, U, Danner, D, Leder, P, Rawlins, D, Rosenfeld, PJ & Kelly, T 1985, 'High-affinity binding site for a specific nuclear protein in the human IgM gene', Nature, vol. 314, no. 6008, pp. 289-292. https://doi.org/10.1038/314289a0
Hennighausen L, Siebenlist U, Danner D, Leder P, Rawlins D, Rosenfeld PJ et al. High-affinity binding site for a specific nuclear protein in the human IgM gene. Nature. 1985 Jul 17;314(6008):289-292. https://doi.org/10.1038/314289a0
Hennighausen, L. ; Siebenlist, U. ; Danner, D. ; Leder, P. ; Rawlins, D. ; Rosenfeld, Philip J ; Kelly, T. / High-affinity binding site for a specific nuclear protein in the human IgM gene. In: Nature. 1985 ; Vol. 314, No. 6008. pp. 289-292.
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