Abstract
Human Germinal Center-associated Lymphoma (HGAL) is a germinal center (GC) B-cell marker associated with a favorable outcome in diffuse large B-cell and classic Hodgkin lymphomas (CHL). To test its potential role in GC function, 75 cases involving GC disruption including 23 progressive transformation of germinal centers (PTGC), 25 follicle lysis and 27 Castleman disease (CD) were studied. HGAL protein expression uniformly correlated with GC B-cells in all except a subset of hyaline-vascular CD that showed severe regression of GCs. HGAL staining highlighted dismantled GCs in PTGC, in contrast to weak or absent CD10 and BCL6 staining. In follicle lysis, HGAL staining was comparable to that of CD10, BCL6, and CD21 in highlighting lysed follicles. Our findings show that HGAL protein expression effectively discriminates clusters of GC B-cells in disrupted follicles. Its persistence in disrupted GCs, suggests that it may be necessary for GC maintenance and supports its proposed role of confining B-cells to the GC microenvironment.
| Original language | English |
|---|---|
| Pages (from-to) | 266-272 |
| Number of pages | 7 |
| Journal | Applied Immunohistochemistry and Molecular Morphology |
| Volume | 19 |
| Issue number | 3 |
| DOIs | |
| State | Published - May 1 2011 |
Fingerprint
Keywords
- Castleman disease
- cell motility
- germinal center
- immunohistochemistry
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Medical Laboratory Technology
- Histology
Cite this
HGAL protein expression persists in disorders of germinal center dissolution : Potential role of HGAL in the germinal center microenvironment. / Temmins, Caroline; Zhao, Shuchun; Lossos, Izidore; Natkunam, Yasodha.
In: Applied Immunohistochemistry and Molecular Morphology, Vol. 19, No. 3, 01.05.2011, p. 266-272.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - HGAL protein expression persists in disorders of germinal center dissolution
T2 - Potential role of HGAL in the germinal center microenvironment
AU - Temmins, Caroline
AU - Zhao, Shuchun
AU - Lossos, Izidore
AU - Natkunam, Yasodha
PY - 2011/5/1
Y1 - 2011/5/1
N2 - Human Germinal Center-associated Lymphoma (HGAL) is a germinal center (GC) B-cell marker associated with a favorable outcome in diffuse large B-cell and classic Hodgkin lymphomas (CHL). To test its potential role in GC function, 75 cases involving GC disruption including 23 progressive transformation of germinal centers (PTGC), 25 follicle lysis and 27 Castleman disease (CD) were studied. HGAL protein expression uniformly correlated with GC B-cells in all except a subset of hyaline-vascular CD that showed severe regression of GCs. HGAL staining highlighted dismantled GCs in PTGC, in contrast to weak or absent CD10 and BCL6 staining. In follicle lysis, HGAL staining was comparable to that of CD10, BCL6, and CD21 in highlighting lysed follicles. Our findings show that HGAL protein expression effectively discriminates clusters of GC B-cells in disrupted follicles. Its persistence in disrupted GCs, suggests that it may be necessary for GC maintenance and supports its proposed role of confining B-cells to the GC microenvironment.
AB - Human Germinal Center-associated Lymphoma (HGAL) is a germinal center (GC) B-cell marker associated with a favorable outcome in diffuse large B-cell and classic Hodgkin lymphomas (CHL). To test its potential role in GC function, 75 cases involving GC disruption including 23 progressive transformation of germinal centers (PTGC), 25 follicle lysis and 27 Castleman disease (CD) were studied. HGAL protein expression uniformly correlated with GC B-cells in all except a subset of hyaline-vascular CD that showed severe regression of GCs. HGAL staining highlighted dismantled GCs in PTGC, in contrast to weak or absent CD10 and BCL6 staining. In follicle lysis, HGAL staining was comparable to that of CD10, BCL6, and CD21 in highlighting lysed follicles. Our findings show that HGAL protein expression effectively discriminates clusters of GC B-cells in disrupted follicles. Its persistence in disrupted GCs, suggests that it may be necessary for GC maintenance and supports its proposed role of confining B-cells to the GC microenvironment.
KW - Castleman disease
KW - cell motility
KW - germinal center
KW - immunohistochemistry
UR - http://www.scopus.com/inward/record.url?scp=79955064210&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79955064210&partnerID=8YFLogxK
U2 - 10.1097/PAI.0b013e3181f89a4d
DO - 10.1097/PAI.0b013e3181f89a4d
M3 - Article
C2 - 21475040
AN - SCOPUS:79955064210
VL - 19
SP - 266
EP - 272
JO - Applied Immunohistochemistry and Molecular Morphology
JF - Applied Immunohistochemistry and Molecular Morphology
SN - 1541-2016
IS - 3
ER -