Heterotypic neuronal differentiation of adult subependymal zone neuronal progenitor cells transplanted to the adult hippocampus

R. Mark Richardson, William C. Broaddus, Kathryn L. Holloway, Dong Sun, Ross Bullock, Helen L. Fillmore

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Neuronal progenitor cells (NPCs) residing in the adult subependymal zone (SEZ) are a potential source of expandable cells for autologous transplantation to replace neurons lost in multiple types of brain injury. To characterize the capacity of these cells for neuronal differentiation in a mature ectopic environment, NPCs expanded from the SEZ of adult rats were transplanted to the adult dentate gyrus. Cultures comprised a heterogeneous population of proliferating cells, which expressed nestin (47%) and GFAP (37%), with many cells expressing both progenitor cell markers (31%). In grafts of undifferentiated cells, as well as in grafts of cells that were induced to differentiate in vitro with retinoic acid, 35% of the transplanted SEZ-derived cells exhibited immunohistochemical and morphological features characteristic of hippocampal granule cell neurons. These novel results indicate that in vitro expanded adult SEZ NPCs are capable of heterotypic neuronal differentiation in a neurogenic region of the adult brain.

Original languageEnglish
Pages (from-to)674-682
Number of pages9
JournalMolecular and Cellular Neuroscience
Volume28
Issue number4
DOIs
StatePublished - Apr 1 2005
Externally publishedYes

Fingerprint

Hippocampus
Stem Cells
Transplants
Neurons
Nestin
Autologous Transplantation
Multiple Trauma
Cell Transplantation
Dentate Gyrus
Tretinoin
Brain Injuries
Cell Differentiation
Brain
Population
In Vitro Techniques

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

Cite this

Heterotypic neuronal differentiation of adult subependymal zone neuronal progenitor cells transplanted to the adult hippocampus. / Richardson, R. Mark; Broaddus, William C.; Holloway, Kathryn L.; Sun, Dong; Bullock, Ross; Fillmore, Helen L.

In: Molecular and Cellular Neuroscience, Vol. 28, No. 4, 01.04.2005, p. 674-682.

Research output: Contribution to journalArticle

Richardson, R. Mark ; Broaddus, William C. ; Holloway, Kathryn L. ; Sun, Dong ; Bullock, Ross ; Fillmore, Helen L. / Heterotypic neuronal differentiation of adult subependymal zone neuronal progenitor cells transplanted to the adult hippocampus. In: Molecular and Cellular Neuroscience. 2005 ; Vol. 28, No. 4. pp. 674-682.
@article{8c5fa765d4814e47a66bed9ccb9b130c,
title = "Heterotypic neuronal differentiation of adult subependymal zone neuronal progenitor cells transplanted to the adult hippocampus",
abstract = "Neuronal progenitor cells (NPCs) residing in the adult subependymal zone (SEZ) are a potential source of expandable cells for autologous transplantation to replace neurons lost in multiple types of brain injury. To characterize the capacity of these cells for neuronal differentiation in a mature ectopic environment, NPCs expanded from the SEZ of adult rats were transplanted to the adult dentate gyrus. Cultures comprised a heterogeneous population of proliferating cells, which expressed nestin (47{\%}) and GFAP (37{\%}), with many cells expressing both progenitor cell markers (31{\%}). In grafts of undifferentiated cells, as well as in grafts of cells that were induced to differentiate in vitro with retinoic acid, 35{\%} of the transplanted SEZ-derived cells exhibited immunohistochemical and morphological features characteristic of hippocampal granule cell neurons. These novel results indicate that in vitro expanded adult SEZ NPCs are capable of heterotypic neuronal differentiation in a neurogenic region of the adult brain.",
author = "Richardson, {R. Mark} and Broaddus, {William C.} and Holloway, {Kathryn L.} and Dong Sun and Ross Bullock and Fillmore, {Helen L.}",
year = "2005",
month = "4",
day = "1",
doi = "10.1016/j.mcn.2004.11.013",
language = "English",
volume = "28",
pages = "674--682",
journal = "Molecular and Cellular Neuroscience",
issn = "1044-7431",
publisher = "Academic Press Inc.",
number = "4",

}

TY - JOUR

T1 - Heterotypic neuronal differentiation of adult subependymal zone neuronal progenitor cells transplanted to the adult hippocampus

AU - Richardson, R. Mark

AU - Broaddus, William C.

AU - Holloway, Kathryn L.

AU - Sun, Dong

AU - Bullock, Ross

AU - Fillmore, Helen L.

PY - 2005/4/1

Y1 - 2005/4/1

N2 - Neuronal progenitor cells (NPCs) residing in the adult subependymal zone (SEZ) are a potential source of expandable cells for autologous transplantation to replace neurons lost in multiple types of brain injury. To characterize the capacity of these cells for neuronal differentiation in a mature ectopic environment, NPCs expanded from the SEZ of adult rats were transplanted to the adult dentate gyrus. Cultures comprised a heterogeneous population of proliferating cells, which expressed nestin (47%) and GFAP (37%), with many cells expressing both progenitor cell markers (31%). In grafts of undifferentiated cells, as well as in grafts of cells that were induced to differentiate in vitro with retinoic acid, 35% of the transplanted SEZ-derived cells exhibited immunohistochemical and morphological features characteristic of hippocampal granule cell neurons. These novel results indicate that in vitro expanded adult SEZ NPCs are capable of heterotypic neuronal differentiation in a neurogenic region of the adult brain.

AB - Neuronal progenitor cells (NPCs) residing in the adult subependymal zone (SEZ) are a potential source of expandable cells for autologous transplantation to replace neurons lost in multiple types of brain injury. To characterize the capacity of these cells for neuronal differentiation in a mature ectopic environment, NPCs expanded from the SEZ of adult rats were transplanted to the adult dentate gyrus. Cultures comprised a heterogeneous population of proliferating cells, which expressed nestin (47%) and GFAP (37%), with many cells expressing both progenitor cell markers (31%). In grafts of undifferentiated cells, as well as in grafts of cells that were induced to differentiate in vitro with retinoic acid, 35% of the transplanted SEZ-derived cells exhibited immunohistochemical and morphological features characteristic of hippocampal granule cell neurons. These novel results indicate that in vitro expanded adult SEZ NPCs are capable of heterotypic neuronal differentiation in a neurogenic region of the adult brain.

UR - http://www.scopus.com/inward/record.url?scp=15244356942&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=15244356942&partnerID=8YFLogxK

U2 - 10.1016/j.mcn.2004.11.013

DO - 10.1016/j.mcn.2004.11.013

M3 - Article

C2 - 15797714

AN - SCOPUS:15244356942

VL - 28

SP - 674

EP - 682

JO - Molecular and Cellular Neuroscience

JF - Molecular and Cellular Neuroscience

SN - 1044-7431

IS - 4

ER -