Heterotopic uterus transplantation in a swine model

Donna L. Avison, Werviston Defaria, Panagiotis Tryphonopoulos, Akin Tekin, George R. Attia, Hidenori Takahashi, Yide Jin, Emmanouil Palaios, Nikolaos Pararas, Manuel R. Carreno, Sergio Santiago, Fuller Bazer, Phillip Ruiz, Andreas Tzakis

Research output: Contribution to journalArticle

71 Scopus citations

Abstract

Background.: The aim of our study was to examine the feasibility of allogeneic uterine transplantation in a large animal model. Methods.: We performed heterotopic uterine transplants in genetically defined mini-pigs. Immunosuppression was tacrolimus administered intravenously for the first 12 days posttransplantation followed by oral cyclosporine maintenance immunosuppression. The graft was transplanted heterotopically in the lower abdominal cavity of the recipient. The vaginal vault was exteriorized as a stoma in the lower right abdominal wall. The uterine grafts were followed with endoscopies and biopsies. Results.: Ten transplants were performed. Follow-up was until July 2008. At the end of the follow-up period, 5 animals were alive and healthy, 0.5 to 12 months posttransplantation. There were 5 deaths due to pneumonia (n=1), intussusception of the graft (n=1), cardiorespiratory arrest during anesthesia (n=1), and complications of the stoma (n=2). Acute rejections of the graft presented during the 2nd and 3rd month posttransplantation were treated successfully with increase of the maintenance immunosuppression and steroids. Other complications included prolapse and infections of the graft stoma. Pathological changes seen in the endometrial biopsies included acute rejection and acute endometritis. Conclusion.: These findings demonstrate that successful uterus transplantation in a large animal model (miniature swine) is feasible using this heterotopic model, and it can be useful for the study of these transplants.

Original languageEnglish (US)
Pages (from-to)465-469
Number of pages5
JournalTransplantation
Volume88
Issue number4
DOIs
StatePublished - Aug 1 2009

Keywords

  • Heterotopic
  • Miniature swine
  • Transplantation
  • Uterus

ASJC Scopus subject areas

  • Transplantation

Fingerprint Dive into the research topics of 'Heterotopic uterus transplantation in a swine model'. Together they form a unique fingerprint.

  • Cite this

    Avison, D. L., Defaria, W., Tryphonopoulos, P., Tekin, A., Attia, G. R., Takahashi, H., Jin, Y., Palaios, E., Pararas, N., Carreno, M. R., Santiago, S., Bazer, F., Ruiz, P., & Tzakis, A. (2009). Heterotopic uterus transplantation in a swine model. Transplantation, 88(4), 465-469. https://doi.org/10.1097/TP.0b013e3181b07666