Abstract
We have analyzed heteroplasmy of mitochondrial DNA in clonal cultures from two patients with Kearns-Sayre syndrome, and have found that individual muscle or fibroblast clones contained either a mixed (i.e. heteroplasmic) population of normal and deleted mitochondrial DNAs, or only normal mitochondrial DNAs (i.e. homoplasmic at a level of detection of <1% deleted genomes). The heteroplasmic clones grew significantly more slowly than did "homoplasmic" clones, probably due to defects of respiratory chain enzymes containing mtDNA-encoded polypeptides.
| Original language | English |
|---|---|
| Pages (from-to) | 765-771 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 160 |
| Issue number | 2 |
| DOIs | |
| State | Published - Apr 28 1989 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
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Heteroplasmy of mitochondrial genomes in clonal cultures from patients with Kearns-Sayre syndrome. / Moraes, Carlos T; Schon, Eric A.; DiMauro, Salvatore; Miranda, Armand F.
In: Biochemical and Biophysical Research Communications, Vol. 160, No. 2, 28.04.1989, p. 765-771.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Heteroplasmy of mitochondrial genomes in clonal cultures from patients with Kearns-Sayre syndrome
AU - Moraes, Carlos T
AU - Schon, Eric A.
AU - DiMauro, Salvatore
AU - Miranda, Armand F.
PY - 1989/4/28
Y1 - 1989/4/28
N2 - We have analyzed heteroplasmy of mitochondrial DNA in clonal cultures from two patients with Kearns-Sayre syndrome, and have found that individual muscle or fibroblast clones contained either a mixed (i.e. heteroplasmic) population of normal and deleted mitochondrial DNAs, or only normal mitochondrial DNAs (i.e. homoplasmic at a level of detection of <1% deleted genomes). The heteroplasmic clones grew significantly more slowly than did "homoplasmic" clones, probably due to defects of respiratory chain enzymes containing mtDNA-encoded polypeptides.
AB - We have analyzed heteroplasmy of mitochondrial DNA in clonal cultures from two patients with Kearns-Sayre syndrome, and have found that individual muscle or fibroblast clones contained either a mixed (i.e. heteroplasmic) population of normal and deleted mitochondrial DNAs, or only normal mitochondrial DNAs (i.e. homoplasmic at a level of detection of <1% deleted genomes). The heteroplasmic clones grew significantly more slowly than did "homoplasmic" clones, probably due to defects of respiratory chain enzymes containing mtDNA-encoded polypeptides.
UR - http://www.scopus.com/inward/record.url?scp=0024321834&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024321834&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(89)92499-6
DO - 10.1016/0006-291X(89)92499-6
M3 - Article
C2 - 2541710
AN - SCOPUS:0024321834
VL - 160
SP - 765
EP - 771
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -