Heterologous complementation of the Klaac null mutation of Kluyveromyces lactis by the Saccharomyces cerevisiae AAC3 gene encoding the ADP/ATP carrier

Flavia Fontanesi, Anna Maria Viola, Iliana Ferrero

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The KlAAC gene, encoding the ADP/ATP carrier, has been assumed to be a single gene in Kluyveromyces lactis, an aerobic, petite-negative yeast species. The Klaac null mutation, which causes a respiratory-deficient phenotype, was fully complemented by AAC2, the Saccharomyces cerevisiae major gene for the ADP/ATP carrier and also by AAC1, a gene that is poorly expressed in S. cerevisiae. In this study, we demonstrate that the Klaac null mutation is partially complemented by the ScAAC3 gene, encoding the hypoxic ADP/ATP carrier isoform, whose expression in S. cerevisiae is prevented by oxygen. Once introduced into K. lactis, the AAC3 gene was expressed both under aerobic and under partial anaerobic conditions but did not support the growth of K. lactis under strict anaerobic conditions.

Original languageEnglish (US)
Pages (from-to)414-420
Number of pages7
JournalFEMS Yeast Research
Volume6
Issue number3
DOIs
StatePublished - May 2006
Externally publishedYes

Fingerprint

ATP Translocases Mitochondrial ADP
Kluyveromyces
Saccharomyces cerevisiae
Mutation
Genes
Protein Isoforms
Yeasts
Oxygen
Phenotype
Growth

Keywords

  • ADP/ATP carrier
  • Anaerobiosis
  • Heterologous complementation
  • Kluyveromyces lactis
  • ScAAC3
  • Semi-anaerobiosis

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology
  • Infectious Diseases

Cite this

Heterologous complementation of the Klaac null mutation of Kluyveromyces lactis by the Saccharomyces cerevisiae AAC3 gene encoding the ADP/ATP carrier. / Fontanesi, Flavia; Viola, Anna Maria; Ferrero, Iliana.

In: FEMS Yeast Research, Vol. 6, No. 3, 05.2006, p. 414-420.

Research output: Contribution to journalArticle

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