Heterogeneous expression of erbb-2 messenger rna in human breast cancer

C. Richter King, Sandra M. Swain, Laura Porter, Seth M. Steinberg, Marc E. Lippman, Edward P. Gelmann

Research output: Contribution to journalArticlepeer-review

52 Scopus citations

Abstract

Amplification and mRNA expression of the erbB-2 gene was analyzed in 61 samples of primary human breast carcinoma. In the 57 samples where RNA could be isolated four different expression level groups were identified. Comparison of hybridization signal with that for β-actin revealed that erbB-2 mRNA could not be detected in 6 of 57 samples (11%), was detected at normal levels in 32 of 57 samples (56%), showed 4- to 8-fold overexpression in 8 of 57 samples (14%), and showed 16- to 128-fold overexpression in 11 of 57 samples (19%). Examination of the DNA of the same set of samples revealed 6 of 61 samples (10%) with distinct gene amplification and 6 of 61 samples (10%) with possible gene amplification. The highest levels of erbB-2 overexpression were associated with gene amplification. Samples with 4- to 16-fold overexpression of the erbB-2 mRNA occurred without evident gene abnormalities. There was no association of erbB-2 expression or gene amplification with clinical stage of breast carcinoma or axillary lymph node involvement. The clear amplification of the erbB-2 gene may be associated with a significantly shorter time to treatment failure.

Original languageEnglish (US)
Pages (from-to)4185-4191
Number of pages7
JournalCancer Research
Volume49
Issue number15
StatePublished - Aug 1 1989

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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