herg encodes a K+ current highly conserved in tumors of different histogenesis: A selective advantage for cancer cells?

Laura Bianchi, Barbara Wible, Annarosa Arcangeli, Maurizio Taglialatela, Ferdinande Morra, Pasqualina Castaldo, Olivia Crociani, Barbara Rosati, Laura Faravelli, Massimo Olivotto, Enzo Wanke

Research output: Contribution to journalArticle

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Abstract

The human ether-a-go-go-related gene (berg) encodes a K+ current (I(HERG)) that plays a fundamental role in heart excitability by regulating the action potential repolarization (I(Kr)); mutations of this gene are responsible for the chromosome 7-linked long QT syndrome (LQT2). In this report, we show that in a variety (n = 17) of tumor cell lines of different species (human and murine) and distinct histogenesis (neuroblastoma, rhabdomyosarcoma, adenocarcinoma, lung microcytoma, pituitary tumors, insulinoma β-cells, and monoblastic leukemia), a novel K+ inward-rectifier current (I(IR)), which is biophysically and pharmacologically similar to I(HERG), can be recorded with the patch-damp technique. Northern blot experiments with a human berg cDNA probe revealed that both in human and murine clones the very high expression of berg transcripts can be quantified in at least three clearly identifiable bands, suggesting an alternative splicing of HERG mRNA. Moreover, we cloned a cDNA encoding for I(IR) from the SH-SY5Y human neuroblastoma. The sequence of this cDNA result was practically identical to that already reported for herg, indicating a high conservation of this gene in tumors. Consistently, the expression of this clone in Xenopus oocytes showed that the encoded K+ channel had substantially all of the biophysical and pharmacological properties of the native I(IR) described for tumor cells. In addition, in the tumor clones studied, I(IR) governs the resting potential, whereas it could not be detected either by the patch clamp or the Northern blot techniques in cells obtained from primary cell cultures of parental tissues (sensory neurons and myotubes), whose resting potential is controlled by the classical K+ anomalous rectifier current. This current substitution had a profound impact on the resting potential, which was markedly depolarized in tumors as compared with normal cells. These results suggest that I(IR) is normally only expressed during the early stages of cell differentiation frozen by neoplastic transformation, playing an important pathophysiological role in the regulatory mechanisms of neoplastic cell survival. In fact, because of its biophysical features, I(IR), besides keeping the resting potential within the depolarized values required for unlimited tumor growth, could also appear suitable to afford a selective advantage in an ischemic environment.

Original languageEnglish
Pages (from-to)815-822
Number of pages8
JournalCancer Research
Volume58
Issue number4
StatePublished - Feb 15 1998
Externally publishedYes

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Membrane Potentials
Complementary DNA
Clone Cells
Neoplasms
Neuroblastoma
Northern Blotting
Genes
Long QT Syndrome
Insulinoma
Chromosomes, Human, Pair 7
Primary Cell Culture
Rhabdomyosarcoma
Skeletal Muscle Fibers
Alternative Splicing
Pituitary Neoplasms
Sensory Receptor Cells
Xenopus
Tumor Cell Line
Ether
Action Potentials

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Bianchi, L., Wible, B., Arcangeli, A., Taglialatela, M., Morra, F., Castaldo, P., ... Wanke, E. (1998). herg encodes a K+ current highly conserved in tumors of different histogenesis: A selective advantage for cancer cells? Cancer Research, 58(4), 815-822.

herg encodes a K+ current highly conserved in tumors of different histogenesis : A selective advantage for cancer cells? / Bianchi, Laura; Wible, Barbara; Arcangeli, Annarosa; Taglialatela, Maurizio; Morra, Ferdinande; Castaldo, Pasqualina; Crociani, Olivia; Rosati, Barbara; Faravelli, Laura; Olivotto, Massimo; Wanke, Enzo.

In: Cancer Research, Vol. 58, No. 4, 15.02.1998, p. 815-822.

Research output: Contribution to journalArticle

Bianchi, L, Wible, B, Arcangeli, A, Taglialatela, M, Morra, F, Castaldo, P, Crociani, O, Rosati, B, Faravelli, L, Olivotto, M & Wanke, E 1998, 'herg encodes a K+ current highly conserved in tumors of different histogenesis: A selective advantage for cancer cells?', Cancer Research, vol. 58, no. 4, pp. 815-822.
Bianchi L, Wible B, Arcangeli A, Taglialatela M, Morra F, Castaldo P et al. herg encodes a K+ current highly conserved in tumors of different histogenesis: A selective advantage for cancer cells? Cancer Research. 1998 Feb 15;58(4):815-822.
Bianchi, Laura ; Wible, Barbara ; Arcangeli, Annarosa ; Taglialatela, Maurizio ; Morra, Ferdinande ; Castaldo, Pasqualina ; Crociani, Olivia ; Rosati, Barbara ; Faravelli, Laura ; Olivotto, Massimo ; Wanke, Enzo. / herg encodes a K+ current highly conserved in tumors of different histogenesis : A selective advantage for cancer cells?. In: Cancer Research. 1998 ; Vol. 58, No. 4. pp. 815-822.
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abstract = "The human ether-a-go-go-related gene (berg) encodes a K+ current (I(HERG)) that plays a fundamental role in heart excitability by regulating the action potential repolarization (I(Kr)); mutations of this gene are responsible for the chromosome 7-linked long QT syndrome (LQT2). In this report, we show that in a variety (n = 17) of tumor cell lines of different species (human and murine) and distinct histogenesis (neuroblastoma, rhabdomyosarcoma, adenocarcinoma, lung microcytoma, pituitary tumors, insulinoma β-cells, and monoblastic leukemia), a novel K+ inward-rectifier current (I(IR)), which is biophysically and pharmacologically similar to I(HERG), can be recorded with the patch-damp technique. Northern blot experiments with a human berg cDNA probe revealed that both in human and murine clones the very high expression of berg transcripts can be quantified in at least three clearly identifiable bands, suggesting an alternative splicing of HERG mRNA. Moreover, we cloned a cDNA encoding for I(IR) from the SH-SY5Y human neuroblastoma. The sequence of this cDNA result was practically identical to that already reported for herg, indicating a high conservation of this gene in tumors. Consistently, the expression of this clone in Xenopus oocytes showed that the encoded K+ channel had substantially all of the biophysical and pharmacological properties of the native I(IR) described for tumor cells. In addition, in the tumor clones studied, I(IR) governs the resting potential, whereas it could not be detected either by the patch clamp or the Northern blot techniques in cells obtained from primary cell cultures of parental tissues (sensory neurons and myotubes), whose resting potential is controlled by the classical K+ anomalous rectifier current. This current substitution had a profound impact on the resting potential, which was markedly depolarized in tumors as compared with normal cells. These results suggest that I(IR) is normally only expressed during the early stages of cell differentiation frozen by neoplastic transformation, playing an important pathophysiological role in the regulatory mechanisms of neoplastic cell survival. In fact, because of its biophysical features, I(IR), besides keeping the resting potential within the depolarized values required for unlimited tumor growth, could also appear suitable to afford a selective advantage in an ischemic environment.",
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AU - Bianchi, Laura

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AU - Taglialatela, Maurizio

AU - Morra, Ferdinande

AU - Castaldo, Pasqualina

AU - Crociani, Olivia

AU - Rosati, Barbara

AU - Faravelli, Laura

AU - Olivotto, Massimo

AU - Wanke, Enzo

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N2 - The human ether-a-go-go-related gene (berg) encodes a K+ current (I(HERG)) that plays a fundamental role in heart excitability by regulating the action potential repolarization (I(Kr)); mutations of this gene are responsible for the chromosome 7-linked long QT syndrome (LQT2). In this report, we show that in a variety (n = 17) of tumor cell lines of different species (human and murine) and distinct histogenesis (neuroblastoma, rhabdomyosarcoma, adenocarcinoma, lung microcytoma, pituitary tumors, insulinoma β-cells, and monoblastic leukemia), a novel K+ inward-rectifier current (I(IR)), which is biophysically and pharmacologically similar to I(HERG), can be recorded with the patch-damp technique. Northern blot experiments with a human berg cDNA probe revealed that both in human and murine clones the very high expression of berg transcripts can be quantified in at least three clearly identifiable bands, suggesting an alternative splicing of HERG mRNA. Moreover, we cloned a cDNA encoding for I(IR) from the SH-SY5Y human neuroblastoma. The sequence of this cDNA result was practically identical to that already reported for herg, indicating a high conservation of this gene in tumors. Consistently, the expression of this clone in Xenopus oocytes showed that the encoded K+ channel had substantially all of the biophysical and pharmacological properties of the native I(IR) described for tumor cells. In addition, in the tumor clones studied, I(IR) governs the resting potential, whereas it could not be detected either by the patch clamp or the Northern blot techniques in cells obtained from primary cell cultures of parental tissues (sensory neurons and myotubes), whose resting potential is controlled by the classical K+ anomalous rectifier current. This current substitution had a profound impact on the resting potential, which was markedly depolarized in tumors as compared with normal cells. These results suggest that I(IR) is normally only expressed during the early stages of cell differentiation frozen by neoplastic transformation, playing an important pathophysiological role in the regulatory mechanisms of neoplastic cell survival. In fact, because of its biophysical features, I(IR), besides keeping the resting potential within the depolarized values required for unlimited tumor growth, could also appear suitable to afford a selective advantage in an ischemic environment.

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