Hepatocyte Growth Factor Treatment Ameliorates Diarrhea and Bowel Inflammation in a Rat Model of Inflammatory Bowel Disease

L. Grier Arthur, Marshall Z. Schwartz, Keith A. Kuenzler, Ruth Birbe, H. Ford, L. G. Arthur

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

Background/Purpose: Transfection of the HLA-B27 gene into normal Fischer rats induces phenotypic changes similar to inflammatory bowel disease (IBD). This study investigated the benefits of 2 doses of hepatocyte growth factor (HGF) on the manifestations of IBD in this rat model. Methods: Fischer rats and HLA-B27 rats were divided into 4 groups: Fischer rats treated with saline, HLA-B27 rats treated with saline, HGF at 150 μg/kg/d, and HGF at 300 μg/kg/d. HGF or saline was infused for 14 days via an osmotic pump attached to a catheter in the internal jugular vein. After treatment, rats were evaluated for diarrhea and reduction in gross and microscopic bowel inflammation. Statistics were determined using analysis of variance (ANOVA). A Pvalue≤ .05 was considered significant. Results: Administration of HGF at 150 μg/kg/d decreased diarrhea by 40%, gross inflammation by 41%, and microscopic inflammation by 72% (P ≤ 5.05). At 300 μg/kg/d HGF decreased diarrhea by 46%, gross inflammation by 45%, and microscopic inflammation by 54% (P ≤ 5.05). Conclusions: HGF administration reduces the clinical manifestations of IBD in this rat model. Similar effects were seen at both doses of HGF administration, implying that there is a plateau above which further increases in HGF levels provides no added benefit. HGF administration may be clinically useful in the management of IBD.

Original languageEnglish (US)
Pages (from-to)139-143
Number of pages5
JournalJournal of Pediatric Surgery
Volume39
Issue number2
DOIs
StatePublished - Feb 2004
Externally publishedYes

Keywords

  • HLA-B27 rats
  • Hepatocyte growth factor
  • Inflammatory bowel disease

ASJC Scopus subject areas

  • Surgery
  • Pediatrics, Perinatology, and Child Health

Fingerprint Dive into the research topics of 'Hepatocyte Growth Factor Treatment Ameliorates Diarrhea and Bowel Inflammation in a Rat Model of Inflammatory Bowel Disease'. Together they form a unique fingerprint.

  • Cite this