Hepatitis C virus RNA quantification in right and left lobes of the liver in patients with chronic hepatitis C

V. Idrovo, P. J. Dailey, Lennox J Jeffers, E. Coelho-Little, D. Bernstein, M. Bartholomew, L. Alvarez, M. S. Urdea, M. L. Collins, Eugene R Schiff

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Quantification of hepatitis C virus RNA in liver tissue is likely to be useful in the study of the natural history, pathogenesis, progression and treatment of hepatitis C virus-associated liver disease. Quantitative measurements of hepatitis C virus RNA in liver biopsy samples using the branched DNA (bDNA) signal amplification assay were carried out. The aims of this study were threefold: first, to assess the level of hepatitis C virus RNA in biopsy samples from the right and left lobes of the liver; second, to evaluate the correlation between hepatitis C virus RNA levels in serum and liver; and third, to investigate the relationship between serum and liver hepatitis C virus RNA levels and the severity of hepatic histology in non-cirrhotic patients with chronic hepatitis C. There was a strong correlation (r = 0.92, P < 0.01) between hepatitis C virus RNA levels in the right and left lobes of the liver as well as a strong correlation between hepatitis C virus RNA levels in liver and serum (r = 0.82, P < 0.01). However, there was no significant correlation between the severity of hepatic histology and levels of hepatitis C virus RNA in serum and liver among patients with chronic active hepatitis classified according to Knodell's hepatic activity index (KI). Our results indicate that hepatitis C virus RNA quantification from a single liver biopsy is representative of both lobes in patients with chronic hepatitis, and suggest that serum hepatitis C virus RNA levels are a meaningful reflection of hepatitis C virus RNA levels in the liver.

Original languageEnglish
Pages (from-to)239-246
Number of pages8
JournalJournal of Viral Hepatitis
Volume3
Issue number5
StatePublished - Sep 1 1996

Fingerprint

Chronic Hepatitis C
Hepacivirus
RNA
Liver
Serum
Chronic Hepatitis
Biopsy
Histology
Branched DNA Signal Amplification Assay
Natural History
Liver Diseases

Keywords

  • Branched DNA (bDNA) signal amplification
  • Hepatitis C virus RNA quantification
  • Laparoscopic-guided liver biopsies
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Hepatology
  • Virology

Cite this

Idrovo, V., Dailey, P. J., Jeffers, L. J., Coelho-Little, E., Bernstein, D., Bartholomew, M., ... Schiff, E. R. (1996). Hepatitis C virus RNA quantification in right and left lobes of the liver in patients with chronic hepatitis C. Journal of Viral Hepatitis, 3(5), 239-246.

Hepatitis C virus RNA quantification in right and left lobes of the liver in patients with chronic hepatitis C. / Idrovo, V.; Dailey, P. J.; Jeffers, Lennox J; Coelho-Little, E.; Bernstein, D.; Bartholomew, M.; Alvarez, L.; Urdea, M. S.; Collins, M. L.; Schiff, Eugene R.

In: Journal of Viral Hepatitis, Vol. 3, No. 5, 01.09.1996, p. 239-246.

Research output: Contribution to journalArticle

Idrovo, V, Dailey, PJ, Jeffers, LJ, Coelho-Little, E, Bernstein, D, Bartholomew, M, Alvarez, L, Urdea, MS, Collins, ML & Schiff, ER 1996, 'Hepatitis C virus RNA quantification in right and left lobes of the liver in patients with chronic hepatitis C', Journal of Viral Hepatitis, vol. 3, no. 5, pp. 239-246.
Idrovo V, Dailey PJ, Jeffers LJ, Coelho-Little E, Bernstein D, Bartholomew M et al. Hepatitis C virus RNA quantification in right and left lobes of the liver in patients with chronic hepatitis C. Journal of Viral Hepatitis. 1996 Sep 1;3(5):239-246.
Idrovo, V. ; Dailey, P. J. ; Jeffers, Lennox J ; Coelho-Little, E. ; Bernstein, D. ; Bartholomew, M. ; Alvarez, L. ; Urdea, M. S. ; Collins, M. L. ; Schiff, Eugene R. / Hepatitis C virus RNA quantification in right and left lobes of the liver in patients with chronic hepatitis C. In: Journal of Viral Hepatitis. 1996 ; Vol. 3, No. 5. pp. 239-246.
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