Hepatitis C virus RNA quantification in right and left lobes of the liver in patients with chronic hepatitis C

V. Idrovo, P. J. Dailey, L. J. Jeffers, E. Coelho-Little, D. Bernstein, M. Bartholomew, L. Alvarez, M. S. Urdea, M. L. Collins, E. R. Schiff

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Quantification of hepatitis C virus RNA in liver tissue is likely to be useful in the study of the natural history, pathogenesis, progression and treatment of hepatitis C virus-associated liver disease. Quantitative measurements of hepatitis C virus RNA in liver biopsy samples using the branched DNA (bDNA) signal amplification assay were carried out. The aims of this study were threefold: first, to assess the level of hepatitis C virus RNA in biopsy samples from the right and left lobes of the liver; second, to evaluate the correlation between hepatitis C virus RNA levels in serum and liver; and third, to investigate the relationship between serum and liver hepatitis C virus RNA levels and the severity of hepatic histology in non-cirrhotic patients with chronic hepatitis C. There was a strong correlation (r = 0.92, P < 0.01) between hepatitis C virus RNA levels in the right and left lobes of the liver as well as a strong correlation between hepatitis C virus RNA levels in liver and serum (r = 0.82, P < 0.01). However, there was no significant correlation between the severity of hepatic histology and levels of hepatitis C virus RNA in serum and liver among patients with chronic active hepatitis classified according to Knodell's hepatic activity index (KI). Our results indicate that hepatitis C virus RNA quantification from a single liver biopsy is representative of both lobes in patients with chronic hepatitis, and suggest that serum hepatitis C virus RNA levels are a meaningful reflection of hepatitis C virus RNA levels in the liver.

Original languageEnglish (US)
Pages (from-to)239-246
Number of pages8
JournalJournal of viral hepatitis
Volume3
Issue number5
DOIs
StatePublished - Sep 1996

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Keywords

  • Branched DNA (bDNA) signal amplification
  • Hepatitis C virus RNA quantification
  • Laparoscopic-guided liver biopsies
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Hepatology
  • Virology

Cite this

Idrovo, V., Dailey, P. J., Jeffers, L. J., Coelho-Little, E., Bernstein, D., Bartholomew, M., Alvarez, L., Urdea, M. S., Collins, M. L., & Schiff, E. R. (1996). Hepatitis C virus RNA quantification in right and left lobes of the liver in patients with chronic hepatitis C. Journal of viral hepatitis, 3(5), 239-246. https://doi.org/10.1111/j.1365-2893.1996.tb00049.x