Abstract
Quantification of hepatitis C virus RNA in liver tissue is likely to be useful in the study of the natural history, pathogenesis, progression and treatment of hepatitis C virus-associated liver disease. Quantitative measurements of hepatitis C virus RNA in liver biopsy samples using the branched DNA (bDNA) signal amplification assay were carried out. The aims of this study were threefold: first, to assess the level of hepatitis C virus RNA in biopsy samples from the right and left lobes of the liver; second, to evaluate the correlation between hepatitis C virus RNA levels in serum and liver; and third, to investigate the relationship between serum and liver hepatitis C virus RNA levels and the severity of hepatic histology in non-cirrhotic patients with chronic hepatitis C. There was a strong correlation (r = 0.92, P < 0.01) between hepatitis C virus RNA levels in the right and left lobes of the liver as well as a strong correlation between hepatitis C virus RNA levels in liver and serum (r = 0.82, P < 0.01). However, there was no significant correlation between the severity of hepatic histology and levels of hepatitis C virus RNA in serum and liver among patients with chronic active hepatitis classified according to Knodell's hepatic activity index (KI). Our results indicate that hepatitis C virus RNA quantification from a single liver biopsy is representative of both lobes in patients with chronic hepatitis, and suggest that serum hepatitis C virus RNA levels are a meaningful reflection of hepatitis C virus RNA levels in the liver.
| Original language | English |
|---|---|
| Pages (from-to) | 239-246 |
| Number of pages | 8 |
| Journal | Journal of Viral Hepatitis |
| Volume | 3 |
| Issue number | 5 |
| State | Published - Sep 1 1996 |
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Keywords
- Branched DNA (bDNA) signal amplification
- Hepatitis C virus RNA quantification
- Laparoscopic-guided liver biopsies
- Polymerase chain reaction
ASJC Scopus subject areas
- Hepatology
- Virology
Cite this
Hepatitis C virus RNA quantification in right and left lobes of the liver in patients with chronic hepatitis C. / Idrovo, V.; Dailey, P. J.; Jeffers, Lennox J; Coelho-Little, E.; Bernstein, D.; Bartholomew, M.; Alvarez, L.; Urdea, M. S.; Collins, M. L.; Schiff, Eugene R.
In: Journal of Viral Hepatitis, Vol. 3, No. 5, 01.09.1996, p. 239-246.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Hepatitis C virus RNA quantification in right and left lobes of the liver in patients with chronic hepatitis C
AU - Idrovo, V.
AU - Dailey, P. J.
AU - Jeffers, Lennox J
AU - Coelho-Little, E.
AU - Bernstein, D.
AU - Bartholomew, M.
AU - Alvarez, L.
AU - Urdea, M. S.
AU - Collins, M. L.
AU - Schiff, Eugene R
PY - 1996/9/1
Y1 - 1996/9/1
N2 - Quantification of hepatitis C virus RNA in liver tissue is likely to be useful in the study of the natural history, pathogenesis, progression and treatment of hepatitis C virus-associated liver disease. Quantitative measurements of hepatitis C virus RNA in liver biopsy samples using the branched DNA (bDNA) signal amplification assay were carried out. The aims of this study were threefold: first, to assess the level of hepatitis C virus RNA in biopsy samples from the right and left lobes of the liver; second, to evaluate the correlation between hepatitis C virus RNA levels in serum and liver; and third, to investigate the relationship between serum and liver hepatitis C virus RNA levels and the severity of hepatic histology in non-cirrhotic patients with chronic hepatitis C. There was a strong correlation (r = 0.92, P < 0.01) between hepatitis C virus RNA levels in the right and left lobes of the liver as well as a strong correlation between hepatitis C virus RNA levels in liver and serum (r = 0.82, P < 0.01). However, there was no significant correlation between the severity of hepatic histology and levels of hepatitis C virus RNA in serum and liver among patients with chronic active hepatitis classified according to Knodell's hepatic activity index (KI). Our results indicate that hepatitis C virus RNA quantification from a single liver biopsy is representative of both lobes in patients with chronic hepatitis, and suggest that serum hepatitis C virus RNA levels are a meaningful reflection of hepatitis C virus RNA levels in the liver.
AB - Quantification of hepatitis C virus RNA in liver tissue is likely to be useful in the study of the natural history, pathogenesis, progression and treatment of hepatitis C virus-associated liver disease. Quantitative measurements of hepatitis C virus RNA in liver biopsy samples using the branched DNA (bDNA) signal amplification assay were carried out. The aims of this study were threefold: first, to assess the level of hepatitis C virus RNA in biopsy samples from the right and left lobes of the liver; second, to evaluate the correlation between hepatitis C virus RNA levels in serum and liver; and third, to investigate the relationship between serum and liver hepatitis C virus RNA levels and the severity of hepatic histology in non-cirrhotic patients with chronic hepatitis C. There was a strong correlation (r = 0.92, P < 0.01) between hepatitis C virus RNA levels in the right and left lobes of the liver as well as a strong correlation between hepatitis C virus RNA levels in liver and serum (r = 0.82, P < 0.01). However, there was no significant correlation between the severity of hepatic histology and levels of hepatitis C virus RNA in serum and liver among patients with chronic active hepatitis classified according to Knodell's hepatic activity index (KI). Our results indicate that hepatitis C virus RNA quantification from a single liver biopsy is representative of both lobes in patients with chronic hepatitis, and suggest that serum hepatitis C virus RNA levels are a meaningful reflection of hepatitis C virus RNA levels in the liver.
KW - Branched DNA (bDNA) signal amplification
KW - Hepatitis C virus RNA quantification
KW - Laparoscopic-guided liver biopsies
KW - Polymerase chain reaction
UR - http://www.scopus.com/inward/record.url?scp=0030225458&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030225458&partnerID=8YFLogxK
M3 - Article
C2 - 8914003
AN - SCOPUS:0030225458
VL - 3
SP - 239
EP - 246
JO - Journal of Viral Hepatitis
JF - Journal of Viral Hepatitis
SN - 1352-0504
IS - 5
ER -