Hepatitis C virus genotypes and viremia and hepatocellular carcinoma in the United States

Andrea E. Reid, Margaret James Koziel, Ignasio Aiza, Lennox J Jeffers, Rajender Reddy, Eugene R Schiff, Johnson Y N Lau, Jules L. Dienstag, T. Jake Liang

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Abstract

OBJECTIVE: Hepatitis C virus (HCV) is a well recognized cause of hepatocellular carcinoma (HCC). The pathogenic significance of HCV genotypes in hepatocarcinogenesis is undefined. The aim of this study was to investigate the genotypic distribution and viremic level of HCV in patients with HCV-associated cirrhosis with or without HCC. METHODS: A total of 28 HCV-infected patients with HCC (HCC+) and 38 patients with HCV-associated cirrhosis without HCC (HCC-) were studied. HCV genotype was assessed by the genotype-specific polymerase chain reaction (PCR) method of Okamoto and restriction fragment length polymorphism (RFLP) of the 5' untranslated region (5' UTR). Hepatitis C viremia was quantitated with the branched-chain DNA (bDNA) assay. RESULTS: Using the Okamoto method, we found genotype lb in 64% of the HCC+ group and 74% of the HCC- group, 36% of the HCC+ group and 16% of the HCC- group were coinfected with a combination of genotype lb and another genotype. Using the RFLP method, we found genotype lb in 41% of the HCC+ group and in 24% of the HCC- group. Other genotypes accounted for 18% of the HCC+ group and 55% of the HCC- group; no combination genotypes were identified. Poor concordance occurred between the two genotyping methods. Mean bDNA levels were not significantly different between the two groups. CONCLUSIONS: Our study demonstrates that no particular HCV genotypes were associated with HCC and genotype did not appear to influence the development of HCV-associated HCC.

Original languageEnglish
Pages (from-to)1619-1626
Number of pages8
JournalAmerican Journal of Gastroenterology
Volume94
Issue number6
DOIs
StatePublished - Jun 1 1999

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Viremia
Hepacivirus
Hepatocellular Carcinoma
Genotype
Restriction Fragment Length Polymorphisms
Fibrosis
Branched DNA Signal Amplification Assay
5' Untranslated Regions
Hepatitis C

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Hepatitis C virus genotypes and viremia and hepatocellular carcinoma in the United States. / Reid, Andrea E.; Koziel, Margaret James; Aiza, Ignasio; Jeffers, Lennox J; Reddy, Rajender; Schiff, Eugene R; Lau, Johnson Y N; Dienstag, Jules L.; Liang, T. Jake.

In: American Journal of Gastroenterology, Vol. 94, No. 6, 01.06.1999, p. 1619-1626.

Research output: Contribution to journalArticle

Reid, AE, Koziel, MJ, Aiza, I, Jeffers, LJ, Reddy, R, Schiff, ER, Lau, JYN, Dienstag, JL & Liang, TJ 1999, 'Hepatitis C virus genotypes and viremia and hepatocellular carcinoma in the United States', American Journal of Gastroenterology, vol. 94, no. 6, pp. 1619-1626. https://doi.org/10.1111/j.1572-0241.1999.01153.x
Reid, Andrea E. ; Koziel, Margaret James ; Aiza, Ignasio ; Jeffers, Lennox J ; Reddy, Rajender ; Schiff, Eugene R ; Lau, Johnson Y N ; Dienstag, Jules L. ; Liang, T. Jake. / Hepatitis C virus genotypes and viremia and hepatocellular carcinoma in the United States. In: American Journal of Gastroenterology. 1999 ; Vol. 94, No. 6. pp. 1619-1626.
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abstract = "OBJECTIVE: Hepatitis C virus (HCV) is a well recognized cause of hepatocellular carcinoma (HCC). The pathogenic significance of HCV genotypes in hepatocarcinogenesis is undefined. The aim of this study was to investigate the genotypic distribution and viremic level of HCV in patients with HCV-associated cirrhosis with or without HCC. METHODS: A total of 28 HCV-infected patients with HCC (HCC+) and 38 patients with HCV-associated cirrhosis without HCC (HCC-) were studied. HCV genotype was assessed by the genotype-specific polymerase chain reaction (PCR) method of Okamoto and restriction fragment length polymorphism (RFLP) of the 5' untranslated region (5' UTR). Hepatitis C viremia was quantitated with the branched-chain DNA (bDNA) assay. RESULTS: Using the Okamoto method, we found genotype lb in 64{\%} of the HCC+ group and 74{\%} of the HCC- group, 36{\%} of the HCC+ group and 16{\%} of the HCC- group were coinfected with a combination of genotype lb and another genotype. Using the RFLP method, we found genotype lb in 41{\%} of the HCC+ group and in 24{\%} of the HCC- group. Other genotypes accounted for 18{\%} of the HCC+ group and 55{\%} of the HCC- group; no combination genotypes were identified. Poor concordance occurred between the two genotyping methods. Mean bDNA levels were not significantly different between the two groups. CONCLUSIONS: Our study demonstrates that no particular HCV genotypes were associated with HCC and genotype did not appear to influence the development of HCV-associated HCC.",
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AU - Koziel, Margaret James

AU - Aiza, Ignasio

AU - Jeffers, Lennox J

AU - Reddy, Rajender

AU - Schiff, Eugene R

AU - Lau, Johnson Y N

AU - Dienstag, Jules L.

AU - Liang, T. Jake

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N2 - OBJECTIVE: Hepatitis C virus (HCV) is a well recognized cause of hepatocellular carcinoma (HCC). The pathogenic significance of HCV genotypes in hepatocarcinogenesis is undefined. The aim of this study was to investigate the genotypic distribution and viremic level of HCV in patients with HCV-associated cirrhosis with or without HCC. METHODS: A total of 28 HCV-infected patients with HCC (HCC+) and 38 patients with HCV-associated cirrhosis without HCC (HCC-) were studied. HCV genotype was assessed by the genotype-specific polymerase chain reaction (PCR) method of Okamoto and restriction fragment length polymorphism (RFLP) of the 5' untranslated region (5' UTR). Hepatitis C viremia was quantitated with the branched-chain DNA (bDNA) assay. RESULTS: Using the Okamoto method, we found genotype lb in 64% of the HCC+ group and 74% of the HCC- group, 36% of the HCC+ group and 16% of the HCC- group were coinfected with a combination of genotype lb and another genotype. Using the RFLP method, we found genotype lb in 41% of the HCC+ group and in 24% of the HCC- group. Other genotypes accounted for 18% of the HCC+ group and 55% of the HCC- group; no combination genotypes were identified. Poor concordance occurred between the two genotyping methods. Mean bDNA levels were not significantly different between the two groups. CONCLUSIONS: Our study demonstrates that no particular HCV genotypes were associated with HCC and genotype did not appear to influence the development of HCV-associated HCC.

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