Heparin inhibits IP3-induced Ca2+ release in permeabilized pancreatic β-cells

Thomas Nilsson; Jean Zwiller; Alton L. Boynton; Per Olof Berggren

doi:10.1016/0014-5793(88)80829-9

Heparin inhibits IP₃-induced Ca²⁺ release in permeabilized pancreatic β-cells

Thomas Nilsson, Jean Zwiller, Alton L. Boynton, Per Olof Berggren

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

Heparin was found to inhibit the Ca²⁺ release induced by inositol 1,4,5-trisphosphate (IP₃) in permeabilized pancreatic β-cells obtained from obese hyperglycemic mice. The effect of heparin was dose-dependent and not due to inhibition of Ca²⁺ uptake into the IP₃-sensitive pool. The effect appeared specific for heparin and was not reproduced by other polysaccharides such as chondroitin sulfates. Heparin might consequently be a useful tool when investigating the molecular mechanism whereby IP₃ mobilizes Ca²⁺.

Original language	English (US)
Pages (from-to)	211-214
Number of pages	4
Journal	FEBS letters
Volume	229
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(88)80829-9
State	Published - Feb 29 1988
Externally published	Yes

Keywords

(Permeabilized pancreatic β-cell)
Ca release
Heparin
Inositol 1,3,4,5-tetrakisphosphate
Inositol 1,4,5-trisphosphate

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

Access to Document

10.1016/0014-5793(88)80829-9

Cite this

@article{27844938164f41c18119e75d7c421209,

title = "Heparin inhibits IP3-induced Ca2+ release in permeabilized pancreatic β-cells",

abstract = "Heparin was found to inhibit the Ca2+ release induced by inositol 1,4,5-trisphosphate (IP3) in permeabilized pancreatic β-cells obtained from obese hyperglycemic mice. The effect of heparin was dose-dependent and not due to inhibition of Ca2+ uptake into the IP3-sensitive pool. The effect appeared specific for heparin and was not reproduced by other polysaccharides such as chondroitin sulfates. Heparin might consequently be a useful tool when investigating the molecular mechanism whereby IP3 mobilizes Ca2+.",

keywords = "(Permeabilized pancreatic β-cell), Ca release, Heparin, Inositol 1,3,4,5-tetrakisphosphate, Inositol 1,4,5-trisphosphate",

author = "Thomas Nilsson and Jean Zwiller and Boynton, {Alton L.} and Berggren, {Per Olof}",

note = "Funding Information: Acknowledgements: This work was supported by grants from the Swedish Medical Research Council (12x-562), the Medical Faculty of Uppsala University, the Swedish Diabetes Association, Novo Industry, Swedish Hoechst, Nordic Insulin, Clas Groschinsky and Family Ernfors Foundations and by NIH/NCI grant CA 39745 and CA 42942. The authors are indebted to Dr R.F. Irvine for his generous gift of IP4.",

year = "1988",

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doi = "10.1016/0014-5793(88)80829-9",

language = "English (US)",

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pages = "211--214",

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T1 - Heparin inhibits IP3-induced Ca2+ release in permeabilized pancreatic β-cells

AU - Nilsson, Thomas

AU - Zwiller, Jean

AU - Boynton, Alton L.

AU - Berggren, Per Olof

N1 - Funding Information: Acknowledgements: This work was supported by grants from the Swedish Medical Research Council (12x-562), the Medical Faculty of Uppsala University, the Swedish Diabetes Association, Novo Industry, Swedish Hoechst, Nordic Insulin, Clas Groschinsky and Family Ernfors Foundations and by NIH/NCI grant CA 39745 and CA 42942. The authors are indebted to Dr R.F. Irvine for his generous gift of IP4.

PY - 1988/2/29

Y1 - 1988/2/29

N2 - Heparin was found to inhibit the Ca2+ release induced by inositol 1,4,5-trisphosphate (IP3) in permeabilized pancreatic β-cells obtained from obese hyperglycemic mice. The effect of heparin was dose-dependent and not due to inhibition of Ca2+ uptake into the IP3-sensitive pool. The effect appeared specific for heparin and was not reproduced by other polysaccharides such as chondroitin sulfates. Heparin might consequently be a useful tool when investigating the molecular mechanism whereby IP3 mobilizes Ca2+.

AB - Heparin was found to inhibit the Ca2+ release induced by inositol 1,4,5-trisphosphate (IP3) in permeabilized pancreatic β-cells obtained from obese hyperglycemic mice. The effect of heparin was dose-dependent and not due to inhibition of Ca2+ uptake into the IP3-sensitive pool. The effect appeared specific for heparin and was not reproduced by other polysaccharides such as chondroitin sulfates. Heparin might consequently be a useful tool when investigating the molecular mechanism whereby IP3 mobilizes Ca2+.

KW - (Permeabilized pancreatic β-cell)

KW - Ca release

KW - Heparin

KW - Inositol 1,3,4,5-tetrakisphosphate

KW - Inositol 1,4,5-trisphosphate

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