Heparin inhibits inositol trisphosphate-induced calcium release from permeabilized rat liver cells

Timothy D. Hill, Per Olof Berggren, Alton L. Boynton

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

Neoplastic rat liver epithelial (261B) cells made permeable by electroporation released 0.2-0.3 μM Ca2+ from intracellular stores in response to 0.5 μM Ins(1,4,5)P3 stimulation. This Ca2+ release response was found to be inhibited by heparin in a dose-dependent manner (Ki of 15 μg/ml). Two other glycosaminoglycans, chondroitin sulfate and hyaluronic acid, showed no inhibitory effect at doses as high as 0.2 mg/ml. Passive Ca2+ release, and sequestration of Ca2+ into intracellular storage sites by the action of Ca2+-ATPase were unaffected by heparin treatment. We conclude that the inhibitory action of heparin treatment on Ca2+ mobilization in permeabilized 261B cells is mediated through its interaction at the Ins(1,4,5)P3 receptor binding site.

Original languageEnglish
Pages (from-to)897-901
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume149
Issue number3
DOIs
StatePublished - Dec 31 1987
Externally publishedYes

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Inositol
Liver
Heparin
Rats
Calcium
Electroporation
Calcium-Transporting ATPases
Hyaluronic Acid
Epithelial Cells
Binding Sites

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Heparin inhibits inositol trisphosphate-induced calcium release from permeabilized rat liver cells. / Hill, Timothy D.; Berggren, Per Olof; Boynton, Alton L.

In: Biochemical and Biophysical Research Communications, Vol. 149, No. 3, 31.12.1987, p. 897-901.

Research output: Contribution to journalArticle

Hill, Timothy D. ; Berggren, Per Olof ; Boynton, Alton L. / Heparin inhibits inositol trisphosphate-induced calcium release from permeabilized rat liver cells. In: Biochemical and Biophysical Research Communications. 1987 ; Vol. 149, No. 3. pp. 897-901.
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