Hematological and acid-base changes in men during prolonged exercise with and without sodium-lactate infusion

Benjamin F. Miller, Michael I. Lindinger, Jill A. Fattor, Kevin Jacobs, Paul J. LeBlanc, Mylinh Duong, George J F Heigenhauser, George A. Brooks

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

An emerging technique used for the study of metabolic regulation is the elevation of lactate concentration with a sodium-lactate infusion, the lactate clamp (LC). However, hematological and acid-base properties affected by the infusion of hypertonic solutions containing the osmotically active strong ions sodium (Na+) and lactate (Lac-) are a concern for clinical and research applications of LC. In the present study, we characterized the hematological and plasma acid-base changes during rest and prolonged, light- to moderate-intensity (55% V̇O2 peak) exercise with and without LC. During the control (Con) trial, subjects were administered an isotonic, isovolumetric saline infusion. During LC, plasma lactate concentration ([Lac-]) was elevated to 4 meq/l during rest and to 4-7 meq/l during exercise. During LC at rest, there were rapid and transient changes in plasma, erythrocyte, and blood volumes. LC resulted in decreased plasma [H+] (from 39.6 to 29.6 neq/l) at the end of exercise while plasma [HCO 3-] increased from 26 to 32.9 meq/l. Increased plasma strong ion difference [SID], due to increased [Na+], was the primary contributor to decreased [H+] and increased [HCO3 -]. A decrease in plasma total weak acid concentration also contributed to these changes, whereas PCO2 contributed little. The infusion of hypertonic LC caused only minor volume, acid-base, and CO 2 storage responses. We conclude that an LC infusion is appropriate for studies of metabolic regulation.

Original languageEnglish
Pages (from-to)856-865
Number of pages10
JournalJournal of Applied Physiology
Volume98
Issue number3
DOIs
StatePublished - Mar 1 2005
Externally publishedYes

Fingerprint

Sodium Lactate
Lactic Acid
Exercise
Acids
Ions
Erythrocyte Volume
Hypertonic Solutions
Plasma Volume
Carbon Monoxide

Keywords

  • Lactate infusion
  • Lactate transport
  • Plasma volume
  • Stewart model
  • Strong ion difference

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Hematological and acid-base changes in men during prolonged exercise with and without sodium-lactate infusion. / Miller, Benjamin F.; Lindinger, Michael I.; Fattor, Jill A.; Jacobs, Kevin; LeBlanc, Paul J.; Duong, Mylinh; Heigenhauser, George J F; Brooks, George A.

In: Journal of Applied Physiology, Vol. 98, No. 3, 01.03.2005, p. 856-865.

Research output: Contribution to journalArticle

Miller, BF, Lindinger, MI, Fattor, JA, Jacobs, K, LeBlanc, PJ, Duong, M, Heigenhauser, GJF & Brooks, GA 2005, 'Hematological and acid-base changes in men during prolonged exercise with and without sodium-lactate infusion', Journal of Applied Physiology, vol. 98, no. 3, pp. 856-865. https://doi.org/10.1152/japplphysiol.00753.2004
Miller, Benjamin F. ; Lindinger, Michael I. ; Fattor, Jill A. ; Jacobs, Kevin ; LeBlanc, Paul J. ; Duong, Mylinh ; Heigenhauser, George J F ; Brooks, George A. / Hematological and acid-base changes in men during prolonged exercise with and without sodium-lactate infusion. In: Journal of Applied Physiology. 2005 ; Vol. 98, No. 3. pp. 856-865.
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