HEMA hydrogels as a substratum for culture of inner ear and statoacoustic ganglion expiants

Zhou Xiang-Ning, Thomas R. Van De Water

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The application of 2-hydroxyethylmethacrylate (HEMA) hydrogels in preparing defined culture substrata for the study of inner ear and statoacoustic ganglion-target tissue expiants was evaluated. Addition of extracellular matrix proteins (collagen type I or fibronectin) to the HEMA hydrogels was effective in promoting nerve fiber outgrowth from statoacoustic ganglion-target tissue expiants. These results although preliminary, demonstrate the property of HEMA hydrogels to embed extracellular matrix components. This ability of the hydrogel culture substratum may be important for the assessment of neurite-promoting activities of extracellular matrix components (e.g. heparan sulfate proteoglycan, laminin, etc.) on in vitro developing statoacoustic ganglion neurons.

Original languageEnglish (US)
Pages (from-to)183-191
Number of pages9
JournalHearing Research
Volume27
Issue number2
DOIs
StatePublished - 1987

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Keywords

  • Extracellular matrix
  • Hydrogel
  • Inner ear
  • Statoacoustic ganglion

ASJC Scopus subject areas

  • Sensory Systems

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