Helicobacter pylori products upregulate neutrophil superoxide anion production.

S. D. Tennenberg, N. Dekhne, D. Gordon, J. Weller, B. McCurdy, P. Lange, Robert Kozol

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Helicobacter pylori-neutrophil interactions may play a pathogenic role in H. pylori-induced gastritis and peptic ulcer disease. To understand these interactions, we explored the effects of H. pylori-derived products on neutrophil chemotaxis and superoxide anion production. H. pylori bacteria were cultured and supernatants fractionated. Neutrophil chemotactic activity was confirmed in the crude supernatants and in one fractionated peak corresponding to a previously described neutrophil chemotactic factor. H. pylori-derived crude supernatant, sonicate and all chromatography-derived peaks failed to directly stimulate neutrophil superoxide anion production. However, after pretreatment with sonicate, neutrophils demonstrated increased superoxide anion production (priming) following subsequent exposure to the secretagogue fmet-leu-phe. These results suggest that H. pylori products may attract neutrophils to the gastric mucosa without initially stimulating superoxide anion production or tissue injury. Oxygen radical-mediated gastric mucosal injury may subsequently result when these primed neutrophils undergo additional stimulation by as yet unidentified factors.

Original languageEnglish
Pages (from-to)301-306
Number of pages6
JournalInternational journal of surgical investigation
Volume1
Issue number4
StatePublished - Jan 1 1999
Externally publishedYes

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Helicobacter pylori
Superoxides
Neutrophils
Up-Regulation
N-Formylmethionine Leucyl-Phenylalanine
Wounds and Injuries
Gastritis
Chemotaxis
Gastric Mucosa
Peptic Ulcer
Interleukin-8
Chromatography
Reactive Oxygen Species
Stomach
Bacteria

Cite this

Tennenberg, S. D., Dekhne, N., Gordon, D., Weller, J., McCurdy, B., Lange, P., & Kozol, R. (1999). Helicobacter pylori products upregulate neutrophil superoxide anion production. International journal of surgical investigation, 1(4), 301-306.

Helicobacter pylori products upregulate neutrophil superoxide anion production. / Tennenberg, S. D.; Dekhne, N.; Gordon, D.; Weller, J.; McCurdy, B.; Lange, P.; Kozol, Robert.

In: International journal of surgical investigation, Vol. 1, No. 4, 01.01.1999, p. 301-306.

Research output: Contribution to journalArticle

Tennenberg, SD, Dekhne, N, Gordon, D, Weller, J, McCurdy, B, Lange, P & Kozol, R 1999, 'Helicobacter pylori products upregulate neutrophil superoxide anion production.', International journal of surgical investigation, vol. 1, no. 4, pp. 301-306.
Tennenberg SD, Dekhne N, Gordon D, Weller J, McCurdy B, Lange P et al. Helicobacter pylori products upregulate neutrophil superoxide anion production. International journal of surgical investigation. 1999 Jan 1;1(4):301-306.
Tennenberg, S. D. ; Dekhne, N. ; Gordon, D. ; Weller, J. ; McCurdy, B. ; Lange, P. ; Kozol, Robert. / Helicobacter pylori products upregulate neutrophil superoxide anion production. In: International journal of surgical investigation. 1999 ; Vol. 1, No. 4. pp. 301-306.
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