Helicobacter pylori products upregulate neutrophil superoxide anion production.

S. D. Tennenberg, N. Dekhne, D. Gordon, J. Weller, B. McCurdy, P. Lange, R. A. Kozol

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Helicobacter pylori-neutrophil interactions may play a pathogenic role in H. pylori-induced gastritis and peptic ulcer disease. To understand these interactions, we explored the effects of H. pylori-derived products on neutrophil chemotaxis and superoxide anion production. H. pylori bacteria were cultured and supernatants fractionated. Neutrophil chemotactic activity was confirmed in the crude supernatants and in one fractionated peak corresponding to a previously described neutrophil chemotactic factor. H. pylori-derived crude supernatant, sonicate and all chromatography-derived peaks failed to directly stimulate neutrophil superoxide anion production. However, after pretreatment with sonicate, neutrophils demonstrated increased superoxide anion production (priming) following subsequent exposure to the secretagogue fmet-leu-phe. These results suggest that H. pylori products may attract neutrophils to the gastric mucosa without initially stimulating superoxide anion production or tissue injury. Oxygen radical-mediated gastric mucosal injury may subsequently result when these primed neutrophils undergo additional stimulation by as yet unidentified factors.

Original languageEnglish (US)
Pages (from-to)301-306
Number of pages6
JournalInternational journal of surgical investigation
Issue number4
StatePublished - 1999

ASJC Scopus subject areas

  • Medicine(all)


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