TY - JOUR
T1 - Heat treatment increases the incidence of alopecia areata in the C3H/HeJ mouse model
AU - Wikramanayake, Tongyu Cao
AU - Alvarez-Connelly, Elizabeth
AU - Simon, Jessica
AU - Mauro, Lucia M.
AU - Guzman, Javier
AU - Elgart, George
AU - Schachner, Lawrence A.
AU - Chen, Juan
AU - Plano, Lisa R.
AU - Jimenez, Joaquin J.
N1 - Funding Information:
Acknowledgments We gratefully acknowledge the Locks of Love Foundation (J.J.J.) and the Brian V. Jegasothy M.D. Basic Science Research Award (T.C.W.) for their support in this investigation. T.C.W. is supported by a Career Development Award from NIH/NIAMS (AR-050487). The authors would like to thank Jie Li, M.D., Ph.D., Ling Tang, Ph.D., Brenda Roberts, Ph.D., and Carmen I. Perez for assistance.
PY - 2010/11
Y1 - 2010/11
N2 - Alopecia areata (AA) is a common autoimmune disease characterized by non-scarring hair loss. Previous studies have demonstrated an association between AA and physiological/psychological stress. In this study, we investigated the effects of heat treatment, a physiological stress, on AA development in C3H/HeJ mice. Whereas this strain of mice are predisposed to AA at low incidence by 18 months of age, we observed a significant increase in the incidence of hair loss in heat-treated 8-month-old C3H/HeJ mice compared with sham-treated mice. Histological analysis detected mononuclear cell infiltration in anagen hair follicles, a characteristic of AA, in heat-treated mouse skin. As expected, increased expression of induced HSPA1A/B (formerly called HSP70i) was detected in skin samples from heat-treated mice. Importantly, increased HSPA1A/B expression was also detected in skin samples from C3H/HeJ mice that developed AA spontaneously. Our results suggest that induction of HSPA1A/B may precipitate the development of AA in C3H/HeJ mice. For future studies, the C3H/HeJ mice with heat treatment may prove a useful model to investigate stress response in AA.
AB - Alopecia areata (AA) is a common autoimmune disease characterized by non-scarring hair loss. Previous studies have demonstrated an association between AA and physiological/psychological stress. In this study, we investigated the effects of heat treatment, a physiological stress, on AA development in C3H/HeJ mice. Whereas this strain of mice are predisposed to AA at low incidence by 18 months of age, we observed a significant increase in the incidence of hair loss in heat-treated 8-month-old C3H/HeJ mice compared with sham-treated mice. Histological analysis detected mononuclear cell infiltration in anagen hair follicles, a characteristic of AA, in heat-treated mouse skin. As expected, increased expression of induced HSPA1A/B (formerly called HSP70i) was detected in skin samples from heat-treated mice. Importantly, increased HSPA1A/B expression was also detected in skin samples from C3H/HeJ mice that developed AA spontaneously. Our results suggest that induction of HSPA1A/B may precipitate the development of AA in C3H/HeJ mice. For future studies, the C3H/HeJ mice with heat treatment may prove a useful model to investigate stress response in AA.
KW - Alopecia areata
KW - C3H/HeJ
KW - Heat shock
KW - Stress
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U2 - 10.1007/s12192-010-0209-7
DO - 10.1007/s12192-010-0209-7
M3 - Article
C2 - 20582641
AN - SCOPUS:78149469723
VL - 15
SP - 985
EP - 991
JO - Cell Stress and Chaperones
JF - Cell Stress and Chaperones
SN - 1355-8145
IS - 6
ER -