Heat shock protein-90 dampens and directs signaling stimulated by insulin-like growth factor-1 and insulin

Gordon P. Meares, Anna A. Zmijewska, Richard S. Jope

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Heat shock protein-90 (Hsp90) buffers cells from genetic mutations and environmental stresses. To test if this capability reflects a normal physiological function of Hsp90 to buffer cellular signals, the effects of Hsp90 inhibition were measured on activation of Akt. Inhibition of Hsp90 with geldanamycin amplified Akt phosphorylation induced by insulin-like growth factor-1 (IGF-1) or insulin, indicating that Hsp90 normally buffers these signals. Furthermore, with IGF-1 stimulation Hsp90 inhibition increased p38 activation, produced additive activation of p90RSK, and slightly increased the duration of ERK1/2 activation. Hsp90 dampened Akt signaling by facilitating phosphatase-mediated dephosphorylation of Akt. Thus, Hsp90 not only buffers the cellular effects of mutations and stresses, but also buffers the magnitude and duration of activation of proliferative and survival-promoting signaling responses.

Original languageEnglish (US)
Pages (from-to)181-186
Number of pages6
JournalFEBS letters
Volume574
Issue number1-3
DOIs
StatePublished - Sep 10 2004
Externally publishedYes

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Keywords

  • Akt
  • Heat shock protein-90
  • Insulin
  • Insulin-like growth factor-1
  • Protein kinase B

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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