HCO3- dehydration by the blood of an elasmobranch in the absence of a Haldane effect

Chris M. Wood, Steve F. Perry, Patrick J. Walsh, Serge Thomas

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

We measured in vivo arterial PCO2 and CaCO2 in Scyliorhinus canicula and found them to be very low (∼ 1 Torr and 3 mmol 1-1 respectively). In vitro, the Haldane effect was functionally absent, and there was no detectable β-adrenergic Na+/H+ exchange, in contrast to teleosts. The HCO3- dehydration rate of the blood, measured by a radioisotopic assay (Wood and Perry, J. Exp. Biol. 157 : 349-366, 1991), was indenpendent of steady-state deoxygenation or oxygenation, unaffected by rapid oxygenation, and insensitive to isoprenaline, amiloride, and removal of urea or TMAO. SITS and acetazolamide reduced the rate; HCO3-/Cl- exchange rather than intracellular carbonic anhydrase (CA) was the rate-limiting factor. The rate was not altered by steady-state plasma [HCO3-], but increased linearly with PCO2 and the RBC concentration, saturating at hematocrits ≥ 15%. The rate in separated plasma accounted for ∼ 50% of the whole blood rate, was higher than in trout plasma or saline, and was inhibited by acetazolamide. The presence of CA in the normally circulating blood plasma of dogfish may contribute to highly effecient CO2 excretion in vivo.

Original languageEnglish
Pages (from-to)319-337
Number of pages19
JournalRespiration Physiology
Volume98
Issue number3
DOIs
StatePublished - Jan 1 1994

Fingerprint

Elasmobranchii
Dehydration
Acetazolamide
Carbonic Anhydrases
4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid
Dogfish
Trout
Amiloride
Hematocrit
Isoproterenol
Adrenergic Agents
Urea

Keywords

  • Blood, CO hydration/dehydration rate
  • Carbon dioxide, blood HCO conversion rate, red cell exchange
  • Fish, dogfish (Scyliorhinus canicula)
  • Protein, band 3
  • Red blood cell, CO exchange

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine

Cite this

HCO3- dehydration by the blood of an elasmobranch in the absence of a Haldane effect. / Wood, Chris M.; Perry, Steve F.; Walsh, Patrick J.; Thomas, Serge.

In: Respiration Physiology, Vol. 98, No. 3, 01.01.1994, p. 319-337.

Research output: Contribution to journalArticle

Wood, Chris M. ; Perry, Steve F. ; Walsh, Patrick J. ; Thomas, Serge. / HCO3- dehydration by the blood of an elasmobranch in the absence of a Haldane effect. In: Respiration Physiology. 1994 ; Vol. 98, No. 3. pp. 319-337.
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