Haptoglobin quantitation in serum samples from clinically normal and clinically abnormal horses

Carolyn Cray, Rodney L. Belgrave

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Haptoglobin (HP) is a common minor to moderate acute-phase protein in mammals. It has been described as increasing under a variety of conditions in horses, with the use of different assay methods. The goal of the current study was to provide updated information regarding this biomarker by using a commonly available automated assay. In the present study, reference intervals for HP were generated using 43 serum samples from clinically normal horses. The reference interval was determined via the robust method as 0.29-2.26 mg/ml. A statistically significant 3.3-fold mean increase was observed in HP levels from a clinically abnormal group of horses (n=33). A weak but significant correlation was found between total white blood cell count and HP levels (r=0.37, P<.05). A significantly higher level of HP expression was observed in samples acquired from patients whose clinical signs lasted for 7 days or longer than from those whose signs were 4 days or less.

Original languageEnglish
Pages (from-to)337-340
Number of pages4
JournalJournal of Equine Veterinary Science
Volume34
Issue number2
DOIs
StatePublished - Feb 1 2014

Fingerprint

Haptoglobins
haptoglobins
Horses
horses
Serum
sampling
acute phase proteins
Acute-Phase Proteins
assays
Leukocyte Count
leukocyte count
Mammals
biomarkers
Biomarkers
mammals
methodology

Keywords

  • Acute-phase protein
  • Acute-phase response
  • Equine
  • Haptoglobin
  • Inflammation

ASJC Scopus subject areas

  • Equine

Cite this

Haptoglobin quantitation in serum samples from clinically normal and clinically abnormal horses. / Cray, Carolyn; Belgrave, Rodney L.

In: Journal of Equine Veterinary Science, Vol. 34, No. 2, 01.02.2014, p. 337-340.

Research output: Contribution to journalArticle

@article{6972c8b9f2284dfdb3c52f39249acc74,
title = "Haptoglobin quantitation in serum samples from clinically normal and clinically abnormal horses",
abstract = "Haptoglobin (HP) is a common minor to moderate acute-phase protein in mammals. It has been described as increasing under a variety of conditions in horses, with the use of different assay methods. The goal of the current study was to provide updated information regarding this biomarker by using a commonly available automated assay. In the present study, reference intervals for HP were generated using 43 serum samples from clinically normal horses. The reference interval was determined via the robust method as 0.29-2.26 mg/ml. A statistically significant 3.3-fold mean increase was observed in HP levels from a clinically abnormal group of horses (n=33). A weak but significant correlation was found between total white blood cell count and HP levels (r=0.37, P<.05). A significantly higher level of HP expression was observed in samples acquired from patients whose clinical signs lasted for 7 days or longer than from those whose signs were 4 days or less.",
keywords = "Acute-phase protein, Acute-phase response, Equine, Haptoglobin, Inflammation",
author = "Carolyn Cray and Belgrave, {Rodney L.}",
year = "2014",
month = "2",
day = "1",
doi = "10.1016/j.jevs.2013.05.007",
language = "English",
volume = "34",
pages = "337--340",
journal = "Journal of Equine Veterinary Science",
issn = "0737-0806",
publisher = "W.B. Saunders Ltd",
number = "2",

}

TY - JOUR

T1 - Haptoglobin quantitation in serum samples from clinically normal and clinically abnormal horses

AU - Cray, Carolyn

AU - Belgrave, Rodney L.

PY - 2014/2/1

Y1 - 2014/2/1

N2 - Haptoglobin (HP) is a common minor to moderate acute-phase protein in mammals. It has been described as increasing under a variety of conditions in horses, with the use of different assay methods. The goal of the current study was to provide updated information regarding this biomarker by using a commonly available automated assay. In the present study, reference intervals for HP were generated using 43 serum samples from clinically normal horses. The reference interval was determined via the robust method as 0.29-2.26 mg/ml. A statistically significant 3.3-fold mean increase was observed in HP levels from a clinically abnormal group of horses (n=33). A weak but significant correlation was found between total white blood cell count and HP levels (r=0.37, P<.05). A significantly higher level of HP expression was observed in samples acquired from patients whose clinical signs lasted for 7 days or longer than from those whose signs were 4 days or less.

AB - Haptoglobin (HP) is a common minor to moderate acute-phase protein in mammals. It has been described as increasing under a variety of conditions in horses, with the use of different assay methods. The goal of the current study was to provide updated information regarding this biomarker by using a commonly available automated assay. In the present study, reference intervals for HP were generated using 43 serum samples from clinically normal horses. The reference interval was determined via the robust method as 0.29-2.26 mg/ml. A statistically significant 3.3-fold mean increase was observed in HP levels from a clinically abnormal group of horses (n=33). A weak but significant correlation was found between total white blood cell count and HP levels (r=0.37, P<.05). A significantly higher level of HP expression was observed in samples acquired from patients whose clinical signs lasted for 7 days or longer than from those whose signs were 4 days or less.

KW - Acute-phase protein

KW - Acute-phase response

KW - Equine

KW - Haptoglobin

KW - Inflammation

UR - http://www.scopus.com/inward/record.url?scp=84894231628&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84894231628&partnerID=8YFLogxK

U2 - 10.1016/j.jevs.2013.05.007

DO - 10.1016/j.jevs.2013.05.007

M3 - Article

AN - SCOPUS:84894231628

VL - 34

SP - 337

EP - 340

JO - Journal of Equine Veterinary Science

JF - Journal of Equine Veterinary Science

SN - 0737-0806

IS - 2

ER -