Hand-held Raman spectroscopy as a pre-screening tool for archaeological bone

William Pestle, Victoria Brennan, Roger L. Sierra, Erin K. Smith, Benjamin J. Vesper, Geoffrey A. Cordell, Michael D. Colvard

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Bone collagen is the required substrate for a variety of archaeometric analyses, including radiocarbon dating, stable isotope analysis, proteomics, and ancient DNA. Sampling of bone for such analyses is, however, a destructive process, and biomolecular extraction is a time-, labor-, and capital-intensive process. As such, the ability to pre-screen bone for potential collagen level in the field (or in remote museums, storage repositories, or other deployed and austere environments) for archaeological and forensic purposes is highly desirable. Building on previous assessments of hand-held spectroscopic tools and several recent bench top Raman studies, and using a robust selection of well-characterized ancient bone samples, it is demonstrated here that rapid (30s), non-destructive assessment by means of 1064nm Raman spectroscopy can provide a field-deployable means by which to quantify bone collagen content. Specifically, it was found that the 1450cm<sup>-1</sup> to 960cm<sup>-1</sup> peak height ratio can provide quantitative estimates of bone collagen content with an error of ±2.8wt%, and those samples with ratios of greater than 0.1 are uniformly suitable for analysis. Hand-held Raman spectroscopic technology has therefore evolved to the point where field deployment by archaeologists and forensic scientists would be both justified and worthwhile.

Original languageEnglish (US)
Pages (from-to)113-120
Number of pages8
JournalJournal of Archaeological Science
Volume58
DOIs
StatePublished - Jun 1 2015

Fingerprint

museum
labor
ability
Raman Spectroscopy
Screening
Bone Collagen
Archaeological Bone
Raman
time
Radiocarbon Dating
Stable Isotope Analysis
Proteomics
Ancient DNA
Substrate
Sampling
Repository
Labor
Archaeologists
Collagen
Archaeology

Keywords

  • Bone
  • Collagen
  • Radiocarbon
  • Raman
  • Spectroscopy
  • Stable isotope

ASJC Scopus subject areas

  • History
  • Archaeology

Cite this

Pestle, W., Brennan, V., Sierra, R. L., Smith, E. K., Vesper, B. J., Cordell, G. A., & Colvard, M. D. (2015). Hand-held Raman spectroscopy as a pre-screening tool for archaeological bone. Journal of Archaeological Science, 58, 113-120. https://doi.org/10.1016/j.jas.2015.03.027

Hand-held Raman spectroscopy as a pre-screening tool for archaeological bone. / Pestle, William; Brennan, Victoria; Sierra, Roger L.; Smith, Erin K.; Vesper, Benjamin J.; Cordell, Geoffrey A.; Colvard, Michael D.

In: Journal of Archaeological Science, Vol. 58, 01.06.2015, p. 113-120.

Research output: Contribution to journalArticle

Pestle, W, Brennan, V, Sierra, RL, Smith, EK, Vesper, BJ, Cordell, GA & Colvard, MD 2015, 'Hand-held Raman spectroscopy as a pre-screening tool for archaeological bone', Journal of Archaeological Science, vol. 58, pp. 113-120. https://doi.org/10.1016/j.jas.2015.03.027
Pestle, William ; Brennan, Victoria ; Sierra, Roger L. ; Smith, Erin K. ; Vesper, Benjamin J. ; Cordell, Geoffrey A. ; Colvard, Michael D. / Hand-held Raman spectroscopy as a pre-screening tool for archaeological bone. In: Journal of Archaeological Science. 2015 ; Vol. 58. pp. 113-120.
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