Hair cycle-dependent production of ACTH in mouse skin

Andrzej Slominski, Natalia V. Botchkareva, Vladimir A. Botchkarev, Ashok Chakraborty, Thomas Luger, Murat Uenalan, Ralf Paus

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

We investigated the functional determinants of the cutaneous expression of elements of the hypothalamic-pituitary-adrenal axis. In the present work, the presence of adrenocorticotropin (ACTH) peptide in skin of C57/BL6 mouse was demonstrated by reversed-phase HPLC analysis combined with specific radioimmunoassay. ACTH concentration that was low in telogen, increased during anagen in two steps: a rapid phase in anagen I, and a slower rise that reached its peak in anagen VI. Immunofluorescence localized the ACTH antigen to the basal layer of epidermis, outer root sheath of hair follicle and subcutaneous muscle of anagen VI skin. At physiological plasma concentration (10-9 M), ACTH selectively stimulated DNA synthesis in dermis, while pharmacological doses (10-7-10-6 M) inhibited DNA synthesis in both dermis and epidermis. In conclusion, we suggest that local production of ACTH may represent a regulatory element in the control of skin functions including hair growth. Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)147-152
Number of pages6
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1448
Issue number1
DOIs
StatePublished - Nov 19 1998
Externally publishedYes

Keywords

  • Adrenocorticotropin
  • Hair growth
  • Proopiomelanocortin
  • Skin

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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