Hair cycle-dependent production of ACTH in mouse skin

Andrzej Slominski, Natalia V. Botchkareva, Vladimir A. Botchkarev, Ashok Chakraborty, Thomas Luger, Murat Uenalan, Ralf Paus

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

We investigated the functional determinants of the cutaneous expression of elements of the hypothalamic-pituitary-adrenal axis. In the present work, the presence of adrenocorticotropin (ACTH) peptide in skin of C57/BL6 mouse was demonstrated by reversed-phase HPLC analysis combined with specific radioimmunoassay. ACTH concentration that was low in telogen, increased during anagen in two steps: a rapid phase in anagen I, and a slower rise that reached its peak in anagen VI. Immunofluorescence localized the ACTH antigen to the basal layer of epidermis, outer root sheath of hair follicle and subcutaneous muscle of anagen VI skin. At physiological plasma concentration (10-9 M), ACTH selectively stimulated DNA synthesis in dermis, while pharmacological doses (10-7-10-6 M) inhibited DNA synthesis in both dermis and epidermis. In conclusion, we suggest that local production of ACTH may represent a regulatory element in the control of skin functions including hair growth. Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)147-152
Number of pages6
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1448
Issue number1
DOIs
StatePublished - Nov 19 1998
Externally publishedYes

Fingerprint

Hair
Adrenocorticotropic Hormone
Skin
Dermis
Epidermis
Hair Follicle
DNA
Radioimmunoassay
Fluorescent Antibody Technique
High Pressure Liquid Chromatography
Pharmacology
Antigens
Muscles
Peptides
Growth

Keywords

  • Adrenocorticotropin
  • Hair growth
  • Proopiomelanocortin
  • Skin

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Slominski, A., Botchkareva, N. V., Botchkarev, V. A., Chakraborty, A., Luger, T., Uenalan, M., & Paus, R. (1998). Hair cycle-dependent production of ACTH in mouse skin. Biochimica et Biophysica Acta - Molecular Cell Research, 1448(1), 147-152. https://doi.org/10.1016/S0167-4889(98)00124-4

Hair cycle-dependent production of ACTH in mouse skin. / Slominski, Andrzej; Botchkareva, Natalia V.; Botchkarev, Vladimir A.; Chakraborty, Ashok; Luger, Thomas; Uenalan, Murat; Paus, Ralf.

In: Biochimica et Biophysica Acta - Molecular Cell Research, Vol. 1448, No. 1, 19.11.1998, p. 147-152.

Research output: Contribution to journalArticle

Slominski, A, Botchkareva, NV, Botchkarev, VA, Chakraborty, A, Luger, T, Uenalan, M & Paus, R 1998, 'Hair cycle-dependent production of ACTH in mouse skin', Biochimica et Biophysica Acta - Molecular Cell Research, vol. 1448, no. 1, pp. 147-152. https://doi.org/10.1016/S0167-4889(98)00124-4
Slominski A, Botchkareva NV, Botchkarev VA, Chakraborty A, Luger T, Uenalan M et al. Hair cycle-dependent production of ACTH in mouse skin. Biochimica et Biophysica Acta - Molecular Cell Research. 1998 Nov 19;1448(1):147-152. https://doi.org/10.1016/S0167-4889(98)00124-4
Slominski, Andrzej ; Botchkareva, Natalia V. ; Botchkarev, Vladimir A. ; Chakraborty, Ashok ; Luger, Thomas ; Uenalan, Murat ; Paus, Ralf. / Hair cycle-dependent production of ACTH in mouse skin. In: Biochimica et Biophysica Acta - Molecular Cell Research. 1998 ; Vol. 1448, No. 1. pp. 147-152.
@article{d62f4101375549fb846f9a681bfc8559,
title = "Hair cycle-dependent production of ACTH in mouse skin",
abstract = "We investigated the functional determinants of the cutaneous expression of elements of the hypothalamic-pituitary-adrenal axis. In the present work, the presence of adrenocorticotropin (ACTH) peptide in skin of C57/BL6 mouse was demonstrated by reversed-phase HPLC analysis combined with specific radioimmunoassay. ACTH concentration that was low in telogen, increased during anagen in two steps: a rapid phase in anagen I, and a slower rise that reached its peak in anagen VI. Immunofluorescence localized the ACTH antigen to the basal layer of epidermis, outer root sheath of hair follicle and subcutaneous muscle of anagen VI skin. At physiological plasma concentration (10-9 M), ACTH selectively stimulated DNA synthesis in dermis, while pharmacological doses (10-7-10-6 M) inhibited DNA synthesis in both dermis and epidermis. In conclusion, we suggest that local production of ACTH may represent a regulatory element in the control of skin functions including hair growth. Copyright (C) 1998 Elsevier Science B.V.",
keywords = "Adrenocorticotropin, Hair growth, Proopiomelanocortin, Skin",
author = "Andrzej Slominski and Botchkareva, {Natalia V.} and Botchkarev, {Vladimir A.} and Ashok Chakraborty and Thomas Luger and Murat Uenalan and Ralf Paus",
year = "1998",
month = "11",
day = "19",
doi = "10.1016/S0167-4889(98)00124-4",
language = "English (US)",
volume = "1448",
pages = "147--152",
journal = "Biochimica et Biophysica Acta - Molecular Cell Research",
issn = "0167-4889",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Hair cycle-dependent production of ACTH in mouse skin

AU - Slominski, Andrzej

AU - Botchkareva, Natalia V.

AU - Botchkarev, Vladimir A.

AU - Chakraborty, Ashok

AU - Luger, Thomas

AU - Uenalan, Murat

AU - Paus, Ralf

PY - 1998/11/19

Y1 - 1998/11/19

N2 - We investigated the functional determinants of the cutaneous expression of elements of the hypothalamic-pituitary-adrenal axis. In the present work, the presence of adrenocorticotropin (ACTH) peptide in skin of C57/BL6 mouse was demonstrated by reversed-phase HPLC analysis combined with specific radioimmunoassay. ACTH concentration that was low in telogen, increased during anagen in two steps: a rapid phase in anagen I, and a slower rise that reached its peak in anagen VI. Immunofluorescence localized the ACTH antigen to the basal layer of epidermis, outer root sheath of hair follicle and subcutaneous muscle of anagen VI skin. At physiological plasma concentration (10-9 M), ACTH selectively stimulated DNA synthesis in dermis, while pharmacological doses (10-7-10-6 M) inhibited DNA synthesis in both dermis and epidermis. In conclusion, we suggest that local production of ACTH may represent a regulatory element in the control of skin functions including hair growth. Copyright (C) 1998 Elsevier Science B.V.

AB - We investigated the functional determinants of the cutaneous expression of elements of the hypothalamic-pituitary-adrenal axis. In the present work, the presence of adrenocorticotropin (ACTH) peptide in skin of C57/BL6 mouse was demonstrated by reversed-phase HPLC analysis combined with specific radioimmunoassay. ACTH concentration that was low in telogen, increased during anagen in two steps: a rapid phase in anagen I, and a slower rise that reached its peak in anagen VI. Immunofluorescence localized the ACTH antigen to the basal layer of epidermis, outer root sheath of hair follicle and subcutaneous muscle of anagen VI skin. At physiological plasma concentration (10-9 M), ACTH selectively stimulated DNA synthesis in dermis, while pharmacological doses (10-7-10-6 M) inhibited DNA synthesis in both dermis and epidermis. In conclusion, we suggest that local production of ACTH may represent a regulatory element in the control of skin functions including hair growth. Copyright (C) 1998 Elsevier Science B.V.

KW - Adrenocorticotropin

KW - Hair growth

KW - Proopiomelanocortin

KW - Skin

UR - http://www.scopus.com/inward/record.url?scp=0032547960&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032547960&partnerID=8YFLogxK

U2 - 10.1016/S0167-4889(98)00124-4

DO - 10.1016/S0167-4889(98)00124-4

M3 - Article

C2 - 9824688

AN - SCOPUS:0032547960

VL - 1448

SP - 147

EP - 152

JO - Biochimica et Biophysica Acta - Molecular Cell Research

JF - Biochimica et Biophysica Acta - Molecular Cell Research

SN - 0167-4889

IS - 1

ER -