Hair cycle-dependent expression of heat shock proteins in hair follicle epithelium

Hideo Hashizume, Yoshiki Tokura, Masahiro Takigawa, Ralf Paus

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Background: Heat shock proteins (HSPs) have a physiologic function in unstressed cells, which is believed to include a role as a 'molecular chaperone'. The hair cycle is characterized by rhythmic tissue remodeling processes, and is an intriguing model for studying the relation between keratinocyte differentiation and HSP expression under physiologic circumstances. We have therefore studied, by immunofluorescence, the expression of selected HSPs during the murine hair cycle. Methods: The association between hair follicle cycling and the expression of three selected HSPs (HSPs 27, 60, and 72) was examined by immunofluorescence, using the depilation-induced hair cycle of C57BL/6 mice. Results: HSP expression was absent from telogen follicles, and was restricted predominantly to keratinocytes in the bulge and the cycling epithelial portion of the hair follicle during anagen and catagen. Immunoreactivity for HSPs 27, 60, and 72 in the hair bulb increased significantly during anagen VI and the catagen transformation of the follicle, and decreased again abruptly with completion of the catagen-telogen transformation. The expression pattern of HSPs 60 and 72 in situ was cytoplasmic, whereas that of HSP 27 was both cytoplasmic and nuclear. Conclusions: These observations suggest that the synthesis of HSPs by hair bulb keratinocytes is related to the anagen-catagen transformation of the follicle, possibly reflecting keratinocyte apoptosis and/or terminal differentiation in the regressing, cycling portion of the follicle. In addition, the rather proximal localization of HBP expression makes it unlikely that the HSPs examined interact with the more distally located intrafollicular γδ T-cell receptor-positive lymphocytes.

Original languageEnglish (US)
Pages (from-to)587-592
Number of pages6
JournalInternational Journal of Dermatology
Volume36
Issue number8
DOIs
StatePublished - Jan 1 1997
Externally publishedYes

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Hair Follicle
Heat-Shock Proteins
Hair
Epithelium
HSP72 Heat-Shock Proteins
HSP27 Heat-Shock Proteins
Chaperonin 60
Keratinocytes
Fluorescent Antibody Technique
Hair Removal
Molecular Chaperones
T-Cell Antigen Receptor
Inbred C57BL Mouse
Lymphocytes
Apoptosis

ASJC Scopus subject areas

  • Dermatology

Cite this

Hair cycle-dependent expression of heat shock proteins in hair follicle epithelium. / Hashizume, Hideo; Tokura, Yoshiki; Takigawa, Masahiro; Paus, Ralf.

In: International Journal of Dermatology, Vol. 36, No. 8, 01.01.1997, p. 587-592.

Research output: Contribution to journalArticle

Hashizume, Hideo ; Tokura, Yoshiki ; Takigawa, Masahiro ; Paus, Ralf. / Hair cycle-dependent expression of heat shock proteins in hair follicle epithelium. In: International Journal of Dermatology. 1997 ; Vol. 36, No. 8. pp. 587-592.
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