Haarlem 3 is the predominant genotype family in multidrug-resistant and extensively drug-resistant Mycobacterium tuberculosis in the capital of Iran: A 5-year survey

Sharereh Khanipour, Nayereh Ebrahimzadeh, Morteza Masoumi, Fatemeh Sakhaei, Farhad Alinezhad, Elham Safarpour, Abolfazl Fateh, Ali Nour Nematollahi, Alireza Hadizadeh Tasbiti, Mohammad Reza Zolfaghari, Ahmad Reza Bahrmand, Mehdi Mirsaeidi, Fatemeh Rahimi Jamnani, Farzam Vaziri, Seyed Davar Siadat

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The objective of this study was to further understand the genetic diversity of multidrug-resistant (MDR) and extensively drug-resistant (XDR) Mycobacterium tuberculosis isolates prevalent in Tehran, the capital city of Iran. From January 2010 to March 2015, a total of 723 M. tuberculosis strains were isolated from patients with pulmonary tuberculosis (TB). A total of 23 MDR, pre-XDR and XDR M. tuberculosis isolates were genotyped by spoligotyping and 24-loci mycobacterial interspersed repetitive unit-variable-number tandem repeat (MIRU-VNTR) typing. The results showed that the MDR, pre-XDR and XDR M. tuberculosis strains mainly belonged to the Haarlem 3 genotype (11/23; 47.8%), followed by the Beijing family (9/23; 39.1%). In addition, the 23 strains were clustered into 21 genotypes using a 24-loci MIRU-VNTR. In conclusion, Haarlem 3 genotype was the predominant genotype among the isolates from MDR-TB cases in this study, which could be of special concern.

Original languageEnglish (US)
Pages (from-to)7-10
Number of pages4
JournalJournal of Global Antimicrobial Resistance
Volume5
DOIs
StatePublished - Jun 1 2016

Fingerprint

Extensively Drug-Resistant Tuberculosis
Iran
Mycobacterium tuberculosis
Genotype
Minisatellite Repeats
Multidrug-Resistant Tuberculosis
Pulmonary Tuberculosis
Surveys and Questionnaires
Pharmaceutical Preparations

Keywords

  • 24-Loci MIRU-VNTR
  • Haarlem 3
  • MDR-TB
  • Spoligotyping

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Immunology and Allergy
  • Microbiology (medical)

Cite this

Haarlem 3 is the predominant genotype family in multidrug-resistant and extensively drug-resistant Mycobacterium tuberculosis in the capital of Iran : A 5-year survey. / Khanipour, Sharereh; Ebrahimzadeh, Nayereh; Masoumi, Morteza; Sakhaei, Fatemeh; Alinezhad, Farhad; Safarpour, Elham; Fateh, Abolfazl; Nour Nematollahi, Ali; Hadizadeh Tasbiti, Alireza; Zolfaghari, Mohammad Reza; Bahrmand, Ahmad Reza; Mirsaeidi, Mehdi; Rahimi Jamnani, Fatemeh; Vaziri, Farzam; Siadat, Seyed Davar.

In: Journal of Global Antimicrobial Resistance, Vol. 5, 01.06.2016, p. 7-10.

Research output: Contribution to journalArticle

Khanipour, S, Ebrahimzadeh, N, Masoumi, M, Sakhaei, F, Alinezhad, F, Safarpour, E, Fateh, A, Nour Nematollahi, A, Hadizadeh Tasbiti, A, Zolfaghari, MR, Bahrmand, AR, Mirsaeidi, M, Rahimi Jamnani, F, Vaziri, F & Siadat, SD 2016, 'Haarlem 3 is the predominant genotype family in multidrug-resistant and extensively drug-resistant Mycobacterium tuberculosis in the capital of Iran: A 5-year survey', Journal of Global Antimicrobial Resistance, vol. 5, pp. 7-10. https://doi.org/10.1016/j.jgar.2016.01.007
Khanipour, Sharereh ; Ebrahimzadeh, Nayereh ; Masoumi, Morteza ; Sakhaei, Fatemeh ; Alinezhad, Farhad ; Safarpour, Elham ; Fateh, Abolfazl ; Nour Nematollahi, Ali ; Hadizadeh Tasbiti, Alireza ; Zolfaghari, Mohammad Reza ; Bahrmand, Ahmad Reza ; Mirsaeidi, Mehdi ; Rahimi Jamnani, Fatemeh ; Vaziri, Farzam ; Siadat, Seyed Davar. / Haarlem 3 is the predominant genotype family in multidrug-resistant and extensively drug-resistant Mycobacterium tuberculosis in the capital of Iran : A 5-year survey. In: Journal of Global Antimicrobial Resistance. 2016 ; Vol. 5. pp. 7-10.
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abstract = "The objective of this study was to further understand the genetic diversity of multidrug-resistant (MDR) and extensively drug-resistant (XDR) Mycobacterium tuberculosis isolates prevalent in Tehran, the capital city of Iran. From January 2010 to March 2015, a total of 723 M. tuberculosis strains were isolated from patients with pulmonary tuberculosis (TB). A total of 23 MDR, pre-XDR and XDR M. tuberculosis isolates were genotyped by spoligotyping and 24-loci mycobacterial interspersed repetitive unit-variable-number tandem repeat (MIRU-VNTR) typing. The results showed that the MDR, pre-XDR and XDR M. tuberculosis strains mainly belonged to the Haarlem 3 genotype (11/23; 47.8{\%}), followed by the Beijing family (9/23; 39.1{\%}). In addition, the 23 strains were clustered into 21 genotypes using a 24-loci MIRU-VNTR. In conclusion, Haarlem 3 genotype was the predominant genotype among the isolates from MDR-TB cases in this study, which could be of special concern.",
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AU - Masoumi, Morteza

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AU - Fateh, Abolfazl

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AU - Hadizadeh Tasbiti, Alireza

AU - Zolfaghari, Mohammad Reza

AU - Bahrmand, Ahmad Reza

AU - Mirsaeidi, Mehdi

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AU - Vaziri, Farzam

AU - Siadat, Seyed Davar

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N2 - The objective of this study was to further understand the genetic diversity of multidrug-resistant (MDR) and extensively drug-resistant (XDR) Mycobacterium tuberculosis isolates prevalent in Tehran, the capital city of Iran. From January 2010 to March 2015, a total of 723 M. tuberculosis strains were isolated from patients with pulmonary tuberculosis (TB). A total of 23 MDR, pre-XDR and XDR M. tuberculosis isolates were genotyped by spoligotyping and 24-loci mycobacterial interspersed repetitive unit-variable-number tandem repeat (MIRU-VNTR) typing. The results showed that the MDR, pre-XDR and XDR M. tuberculosis strains mainly belonged to the Haarlem 3 genotype (11/23; 47.8%), followed by the Beijing family (9/23; 39.1%). In addition, the 23 strains were clustered into 21 genotypes using a 24-loci MIRU-VNTR. In conclusion, Haarlem 3 genotype was the predominant genotype among the isolates from MDR-TB cases in this study, which could be of special concern.

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