H-2K(k) can influence whether cytotoxic T lymphocytes recognize trinitrophenyl in association with H-2D(k) unique or H-2K(k) and H-2D(k) shared self determinants

H. Fujiwara, T. Tsuchida, R. B. Levy, G. M. Shearer

Research output: Contribution to journalArticlepeer-review

Abstract

The present study investigates the effect of trinitrophenyl- (TNP) modified H-2K(k) (TNP-K(k)) antigens on the generation of anti-TNP-D(k) restricted cytotoxic T lymphocyte (CTL) responses. C3H.OH mice were primed to TNP-self by skin-painting with trinitrochlorobenzene, and spleen cells from these primed mice were subsequently stimulated in vitro with TNP-self. The effector cells generated exhibited appreciable lysis of TNP-modified C3H.OH blast target cells. Cold target inhibition studies demonstrated the generation of two effector cell populations: one that recognizes TNP in association with unique D(k) self determinants, and one that recognizes TNP in association with self determinants shared between TNP-K(k) and TNP-D(k). This was in contrast to primed C3H/He spleen cells, which did not generate CTL that recognized TNP in association with unique D(k) self determinants. When spleen cells from (C3H/He x C3H.OH)F1 mice primed to TNP were stimulated in vitro with TNP-C3H.OH cells, unique D(k) self determinants were recognized in association with TNP. However, in vitro stimulation of the same F1 responding cells with TNP-C3H/He or TNP-F1 cells failed to elicit CTL that utilized these D(k)-unique self determinants. The findings of this study demonstrate that unique or shared H-2D(k) determinants can be differentially utilized by CTL populations, depending on the H-2 alleles expressed by the stimulator cells.

Original languageEnglish (US)
Pages (from-to)1189-1193
Number of pages5
JournalJournal of Immunology
Volume129
Issue number3
StatePublished - Jan 1 1982
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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