The present study investigates the effect of trinitrophenyl- (TNP) modified H-2K(k) (TNP-K(k)) antigens on the generation of anti-TNP-D(k) restricted cytotoxic T lymphocyte (CTL) responses. C3H.OH mice were primed to TNP-self by skin-painting with trinitrochlorobenzene, and spleen cells from these primed mice were subsequently stimulated in vitro with TNP-self. The effector cells generated exhibited appreciable lysis of TNP-modified C3H.OH blast target cells. Cold target inhibition studies demonstrated the generation of two effector cell populations: one that recognizes TNP in association with unique D(k) self determinants, and one that recognizes TNP in association with self determinants shared between TNP-K(k) and TNP-D(k). This was in contrast to primed C3H/He spleen cells, which did not generate CTL that recognized TNP in association with unique D(k) self determinants. When spleen cells from (C3H/He x C3H.OH)F1 mice primed to TNP were stimulated in vitro with TNP-C3H.OH cells, unique D(k) self determinants were recognized in association with TNP. However, in vitro stimulation of the same F1 responding cells with TNP-C3H/He or TNP-F1 cells failed to elicit CTL that utilized these D(k)-unique self determinants. The findings of this study demonstrate that unique or shared H-2D(k) determinants can be differentially utilized by CTL populations, depending on the H-2 alleles expressed by the stimulator cells.
|Original language||English (US)|
|Number of pages||5|
|Journal||Journal of Immunology|
|State||Published - Jan 1 1982|
ASJC Scopus subject areas
- Immunology and Allergy