Spleen cells from H-2k, H-2d, and H-2b mice were compared for their ability to generate primary in vitro cytotoxic responses to syngeneic cells modified with fluorescein isothiocyanate (FITC). All strains tested so far generated effector cells which specifically lysed FITC-but not TNP-self targets. Effector activity of H-2b cells was weaker than that of H-2kand H-2d cells. H-2b spleen cells did not respond to syngeneic stimulating cells modified with low concentrations of FITC, whereas H-2k and H-2d cells generated effector activity even when stimulated by syngeneic cells modified with low concentrations of FITC. The cytotoxic response generated by H-2d spleen cells to FITC-self was equivalent to the response of H-2k mice. Further studies using (H-2k × H-2b)F1 responding cells and F1-modified stimulating cells indicated that the F1 cells generated preferential cytotoxic activity in association with H-2k compared with H-2b self products. The results of this study indicate that H-2-linked genetic factors control the ability of inbred mouse strains to generate cytotoxic effector cells to FITC-self. Therefore, although H-2k and H-2b strains are respective high and low CTL responders to TNP, FITC, and other haptens, the present study demonstrates that H-2d as well as H-2k mice are high responders to FITC.
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