Growth rate and 59Fe uptake in the progeny of Rauscher leukemia virus-infected splenic colony forming units following transplantation

James P. Okunewick, Arnold M. Markoe, Penny Erhard, Eleanore L. Phillips

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Studies of the growth rate and erythropoietic activity in the progeny of transplanted colony forming units (CFU) from normal spleens and Rauscher leukemia virus (RLV)-infected spleens have been carried out. In the terms of over-all increase in splenic mass of the recipients, it was found that when spleen cells were transplanted into lethally irradiated mice, the rate of splenic repopulation by cells derived from RLV-infected CFUs was identical with that from normal CFUs, up to the point where the spleens of the animals receiving normal cells approximated twice normal size. At that point the rate of splenic enlargement began to decrease for transplanted normal CFUs. Conversely, splenic enlargement continued at the same exponential rate in those recipients which had received RLV-infected cells. The data indicate that under optimum growth conditions total splenic repopulation by the progeny of both normal and viral-transformed hematopoietic CFUs will occur in vivo at approximately the same rate, differing primarily only in the ability of the cells to respond to the regulator mechanism which governs organ size. Erythropoietic activity was evaluated in terms of 6 hour radioiron uptakes by the spleens of the recipient mice. On a per milligram of spleen basis it was noted that both types of cell populations show a peak in 59Fe incorporation at the beginning of the second week after transplant. However the level of uptake of 59Fe in the progeny derived from RLV-infected CFUs was clearly lower than that seen from normal cells. This suggests that even though both populations of cells are initialy growing at the same rate, the erythropoietic activity of the progeny from RLV-infected cells is much lower than those from normal cells.

Original languageEnglish (US)
Pages (from-to)489-496
Number of pages8
JournalThe Journal of Laboratory and Clinical Medicine
Volume81
Issue number4
StatePublished - Apr 1973
Externally publishedYes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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