Growth of a human breast cancer cell line in serum-free hormone-supplemented medium

J. C. Allegra, Marc E Lippman

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

ZR-75-1, a human breast cancer cell line, has been grown in hormone- supplemented medium without serum. The factors required for optimal growth include 17β-estradiol, insulin, transferrin, dexamethasone, and L- triiodothyronine. If estradiol, insulin, or L-triiodothyronine is omitted, cells cease division within 7 days, but viability is retained for at least 14 days. Omission of transferrin leads to cell death within 7 days. The cells have been continuously maintained in this environment without morphological alteration or cessation of growth for more than 5 months. Addition of the anti-estrogen, Tamoxifen (10-6 M), inhibited cells below the growth rate seen when estradiol was omitted from the medium, even when Tamoxifen was added 4 days and two medium changes after the removal of estradiol from the medium, thus suggesting an action of Tamoxifen which may be independent of competition with estradiol. The availability of a human breast cancer cell line that can be propagated in hormone-supplemented medium without serum should aid in the study of the mechanisms by which hormones effect cell growth.

Original languageEnglish
Pages (from-to)3823-3829
Number of pages7
JournalCancer Research
Volume38
Issue number11 I
StatePublished - Dec 1 1978
Externally publishedYes

Fingerprint

Estradiol
Hormones
Breast Neoplasms
Cell Line
Tamoxifen
Growth
Serum
Triiodothyronine
Transferrin
Insulin
Cell Division
Dexamethasone
Estrogens
Cell Death

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Allegra, J. C., & Lippman, M. E. (1978). Growth of a human breast cancer cell line in serum-free hormone-supplemented medium. Cancer Research, 38(11 I), 3823-3829.

Growth of a human breast cancer cell line in serum-free hormone-supplemented medium. / Allegra, J. C.; Lippman, Marc E.

In: Cancer Research, Vol. 38, No. 11 I, 01.12.1978, p. 3823-3829.

Research output: Contribution to journalArticle

Allegra, JC & Lippman, ME 1978, 'Growth of a human breast cancer cell line in serum-free hormone-supplemented medium', Cancer Research, vol. 38, no. 11 I, pp. 3823-3829.
Allegra, J. C. ; Lippman, Marc E. / Growth of a human breast cancer cell line in serum-free hormone-supplemented medium. In: Cancer Research. 1978 ; Vol. 38, No. 11 I. pp. 3823-3829.
@article{b8f5e0c99b29412bb6d62cec161ea806,
title = "Growth of a human breast cancer cell line in serum-free hormone-supplemented medium",
abstract = "ZR-75-1, a human breast cancer cell line, has been grown in hormone- supplemented medium without serum. The factors required for optimal growth include 17β-estradiol, insulin, transferrin, dexamethasone, and L- triiodothyronine. If estradiol, insulin, or L-triiodothyronine is omitted, cells cease division within 7 days, but viability is retained for at least 14 days. Omission of transferrin leads to cell death within 7 days. The cells have been continuously maintained in this environment without morphological alteration or cessation of growth for more than 5 months. Addition of the anti-estrogen, Tamoxifen (10-6 M), inhibited cells below the growth rate seen when estradiol was omitted from the medium, even when Tamoxifen was added 4 days and two medium changes after the removal of estradiol from the medium, thus suggesting an action of Tamoxifen which may be independent of competition with estradiol. The availability of a human breast cancer cell line that can be propagated in hormone-supplemented medium without serum should aid in the study of the mechanisms by which hormones effect cell growth.",
author = "Allegra, {J. C.} and Lippman, {Marc E}",
year = "1978",
month = "12",
day = "1",
language = "English",
volume = "38",
pages = "3823--3829",
journal = "Journal of Cancer Research",
issn = "0099-7013",
publisher = "American Association for Cancer Research Inc.",
number = "11 I",

}

TY - JOUR

T1 - Growth of a human breast cancer cell line in serum-free hormone-supplemented medium

AU - Allegra, J. C.

AU - Lippman, Marc E

PY - 1978/12/1

Y1 - 1978/12/1

N2 - ZR-75-1, a human breast cancer cell line, has been grown in hormone- supplemented medium without serum. The factors required for optimal growth include 17β-estradiol, insulin, transferrin, dexamethasone, and L- triiodothyronine. If estradiol, insulin, or L-triiodothyronine is omitted, cells cease division within 7 days, but viability is retained for at least 14 days. Omission of transferrin leads to cell death within 7 days. The cells have been continuously maintained in this environment without morphological alteration or cessation of growth for more than 5 months. Addition of the anti-estrogen, Tamoxifen (10-6 M), inhibited cells below the growth rate seen when estradiol was omitted from the medium, even when Tamoxifen was added 4 days and two medium changes after the removal of estradiol from the medium, thus suggesting an action of Tamoxifen which may be independent of competition with estradiol. The availability of a human breast cancer cell line that can be propagated in hormone-supplemented medium without serum should aid in the study of the mechanisms by which hormones effect cell growth.

AB - ZR-75-1, a human breast cancer cell line, has been grown in hormone- supplemented medium without serum. The factors required for optimal growth include 17β-estradiol, insulin, transferrin, dexamethasone, and L- triiodothyronine. If estradiol, insulin, or L-triiodothyronine is omitted, cells cease division within 7 days, but viability is retained for at least 14 days. Omission of transferrin leads to cell death within 7 days. The cells have been continuously maintained in this environment without morphological alteration or cessation of growth for more than 5 months. Addition of the anti-estrogen, Tamoxifen (10-6 M), inhibited cells below the growth rate seen when estradiol was omitted from the medium, even when Tamoxifen was added 4 days and two medium changes after the removal of estradiol from the medium, thus suggesting an action of Tamoxifen which may be independent of competition with estradiol. The availability of a human breast cancer cell line that can be propagated in hormone-supplemented medium without serum should aid in the study of the mechanisms by which hormones effect cell growth.

UR - http://www.scopus.com/inward/record.url?scp=0018168638&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018168638&partnerID=8YFLogxK

M3 - Article

VL - 38

SP - 3823

EP - 3829

JO - Journal of Cancer Research

JF - Journal of Cancer Research

SN - 0099-7013

IS - 11 I

ER -