Abstract
By using as an experimental system the induction of growth of a cloned, antigen:Ia-reactive helper T cell line by an antigen receptor-specific monoclonal antibody, we demonstrated that growth requires two essential co-factors, exogenously produced IL 1 and endogenously produced IL 2. The primary role of the IL 1 is in the expression of receptors on the T cell surface for IL 2, rather than for promoting the synthesis of IL 2. The use of a clone-specific activating monoclonal antibody at nanogram amounts to activate a cloned helper T cell should allow a detailed characterization of T cell activation via antigen receptor cross-linking.
| Original language | English |
|---|---|
| Pages (from-to) | 1339-1345 |
| Number of pages | 7 |
| Journal | Journal of Immunology |
| Volume | 133 |
| Issue number | 3 |
| State | Published - Oct 11 1984 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
Cite this
Growth of a cloned helper T cell line induced by a monoclonal antibody specific for the antigen receptor : Interleukin 1 is required fo the expression of receptors for interleukin 2. / Kaye, J.; Gillis, S.; Mizel, S. B.; Shevach, E. M.; Malek, Thomas; Dinarello, C. A.; Lachman, L. B.; Janeway, C. A.
In: Journal of Immunology, Vol. 133, No. 3, 11.10.1984, p. 1339-1345.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Growth of a cloned helper T cell line induced by a monoclonal antibody specific for the antigen receptor
T2 - Interleukin 1 is required fo the expression of receptors for interleukin 2
AU - Kaye, J.
AU - Gillis, S.
AU - Mizel, S. B.
AU - Shevach, E. M.
AU - Malek, Thomas
AU - Dinarello, C. A.
AU - Lachman, L. B.
AU - Janeway, C. A.
PY - 1984/10/11
Y1 - 1984/10/11
N2 - By using as an experimental system the induction of growth of a cloned, antigen:Ia-reactive helper T cell line by an antigen receptor-specific monoclonal antibody, we demonstrated that growth requires two essential co-factors, exogenously produced IL 1 and endogenously produced IL 2. The primary role of the IL 1 is in the expression of receptors on the T cell surface for IL 2, rather than for promoting the synthesis of IL 2. The use of a clone-specific activating monoclonal antibody at nanogram amounts to activate a cloned helper T cell should allow a detailed characterization of T cell activation via antigen receptor cross-linking.
AB - By using as an experimental system the induction of growth of a cloned, antigen:Ia-reactive helper T cell line by an antigen receptor-specific monoclonal antibody, we demonstrated that growth requires two essential co-factors, exogenously produced IL 1 and endogenously produced IL 2. The primary role of the IL 1 is in the expression of receptors on the T cell surface for IL 2, rather than for promoting the synthesis of IL 2. The use of a clone-specific activating monoclonal antibody at nanogram amounts to activate a cloned helper T cell should allow a detailed characterization of T cell activation via antigen receptor cross-linking.
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UR - http://www.scopus.com/inward/citedby.url?scp=0021175756&partnerID=8YFLogxK
M3 - Article
C2 - 6235287
AN - SCOPUS:0021175756
VL - 133
SP - 1339
EP - 1345
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 3
ER -