Growth of a cloned helper T cell line induced by a monoclonal antibody specific for the antigen receptor: Interleukin 1 is required fo the expression of receptors for interleukin 2

J. Kaye, S. Gillis, S. B. Mizel, E. M. Shevach, Thomas Malek, C. A. Dinarello, L. B. Lachman, C. A. Janeway

Research output: Contribution to journalArticle

395 Citations (Scopus)

Abstract

By using as an experimental system the induction of growth of a cloned, antigen:Ia-reactive helper T cell line by an antigen receptor-specific monoclonal antibody, we demonstrated that growth requires two essential co-factors, exogenously produced IL 1 and endogenously produced IL 2. The primary role of the IL 1 is in the expression of receptors on the T cell surface for IL 2, rather than for promoting the synthesis of IL 2. The use of a clone-specific activating monoclonal antibody at nanogram amounts to activate a cloned helper T cell should allow a detailed characterization of T cell activation via antigen receptor cross-linking.

Original languageEnglish
Pages (from-to)1339-1345
Number of pages7
JournalJournal of Immunology
Volume133
Issue number3
StatePublished - Oct 11 1984
Externally publishedYes

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Antigen Receptors
Interleukin-2 Receptors
Helper-Inducer T-Lymphocytes
Interleukin-1
Interleukin-2
Monoclonal Antibodies
T-Cell Antigen Receptor
Cell Line
Growth
CD27 Antigens
Histocompatibility Antigens Class II
Clone Cells

ASJC Scopus subject areas

  • Immunology

Cite this

Growth of a cloned helper T cell line induced by a monoclonal antibody specific for the antigen receptor : Interleukin 1 is required fo the expression of receptors for interleukin 2. / Kaye, J.; Gillis, S.; Mizel, S. B.; Shevach, E. M.; Malek, Thomas; Dinarello, C. A.; Lachman, L. B.; Janeway, C. A.

In: Journal of Immunology, Vol. 133, No. 3, 11.10.1984, p. 1339-1345.

Research output: Contribution to journalArticle

Kaye, J. ; Gillis, S. ; Mizel, S. B. ; Shevach, E. M. ; Malek, Thomas ; Dinarello, C. A. ; Lachman, L. B. ; Janeway, C. A. / Growth of a cloned helper T cell line induced by a monoclonal antibody specific for the antigen receptor : Interleukin 1 is required fo the expression of receptors for interleukin 2. In: Journal of Immunology. 1984 ; Vol. 133, No. 3. pp. 1339-1345.
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