Growth of a cloned helper T cell line induced by a monoclonal antibody specific for the antigen receptor: Interleukin 1 is required fo the expression of receptors for interleukin 2

J. Kaye; S. Gillis; S. B. Mizel; E. M. Shevach; T. R. Malek; C. A. Dinarello; L. B. Lachman; C. A. Janeway

Growth of a cloned helper T cell line induced by a monoclonal antibody specific for the antigen receptor: Interleukin 1 is required fo the expression of receptors for interleukin 2

J. Kaye, S. Gillis, S. B. Mizel, E. M. Shevach, T. R. Malek, C. A. Dinarello, L. B. Lachman, C. A. Janeway

Research output: Contribution to journal › Article › peer-review

Abstract

By using as an experimental system the induction of growth of a cloned, antigen:Ia-reactive helper T cell line by an antigen receptor-specific monoclonal antibody, we demonstrated that growth requires two essential co-factors, exogenously produced IL 1 and endogenously produced IL 2. The primary role of the IL 1 is in the expression of receptors on the T cell surface for IL 2, rather than for promoting the synthesis of IL 2. The use of a clone-specific activating monoclonal antibody at nanogram amounts to activate a cloned helper T cell should allow a detailed characterization of T cell activation via antigen receptor cross-linking.

Original language	English (US)
Pages (from-to)	1339-1345
Number of pages	7
Journal	Journal of Immunology
Volume	133
Issue number	3
State	Published - 1984
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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Growth of a cloned helper T cell line induced by a monoclonal antibody specific for the antigen receptor : Interleukin 1 is required fo the expression of receptors for interleukin 2. / Kaye, J.; Gillis, S.; Mizel, S. B.; Shevach, E. M.; Malek, T. R.; Dinarello, C. A.; Lachman, L. B.; Janeway, C. A.

In: Journal of Immunology, Vol. 133, No. 3, 1984, p. 1339-1345.

Research output: Contribution to journal › Article › peer-review

Kaye, J. ; Gillis, S. ; Mizel, S. B. ; Shevach, E. M. ; Malek, T. R. ; Dinarello, C. A. ; Lachman, L. B. ; Janeway, C. A. / Growth of a cloned helper T cell line induced by a monoclonal antibody specific for the antigen receptor : Interleukin 1 is required fo the expression of receptors for interleukin 2. In: Journal of Immunology. 1984 ; Vol. 133, No. 3. pp. 1339-1345.

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abstract = "By using as an experimental system the induction of growth of a cloned, antigen:Ia-reactive helper T cell line by an antigen receptor-specific monoclonal antibody, we demonstrated that growth requires two essential co-factors, exogenously produced IL 1 and endogenously produced IL 2. The primary role of the IL 1 is in the expression of receptors on the T cell surface for IL 2, rather than for promoting the synthesis of IL 2. The use of a clone-specific activating monoclonal antibody at nanogram amounts to activate a cloned helper T cell should allow a detailed characterization of T cell activation via antigen receptor cross-linking.",

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