Growth inhibition of primary keratinocytes following transduction with a novel TGFβ-1 containing retrovirus

Evangelos V. Badiavas, Linda Zhou, Vincent Falanga

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Growth and migration of keratinocytes are known to be affected by the addition of exogenous cytokines, such as TGFβ-1, to culture media. We have developed a retroviral vector, LNTβ-1, that confers constitutive expression of human TGFβ-1 to transduced cells. Keratinocytes were exposed to retroviral particles generated in serum-free media, and infected cells were selected for with Geneticin. Transduced keratinocytes remained in culture as single cells instead of a normally grouped growth pattern. While these transduced keratinocytes survived in culture for several weeks, they did not proliferate and seemed arrested in their growth. Keratinocytes transduced with retrovirus not containing the TGFβ-1 gene appeared normal in their growth pattern. These findings indicate that high-level endogenous expression of TGFβ-1 in keratinocytes can at least inhibit, and possibly arrest, growth.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalJournal of dermatological science
Volume27
Issue number1
DOIs
StatePublished - Jan 1 2001
Externally publishedYes

Keywords

  • Growth inhibition
  • Keratinocyte
  • TGFβ-1

ASJC Scopus subject areas

  • Dermatology

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