Abstract
Growth and migration of keratinocytes are known to be affected by the addition of exogenous cytokines, such as TGFβ-1, to culture media. We have developed a retroviral vector, LNTβ-1, that confers constitutive expression of human TGFβ-1 to transduced cells. Keratinocytes were exposed to retroviral particles generated in serum-free media, and infected cells were selected for with Geneticin. Transduced keratinocytes remained in culture as single cells instead of a normally grouped growth pattern. While these transduced keratinocytes survived in culture for several weeks, they did not proliferate and seemed arrested in their growth. Keratinocytes transduced with retrovirus not containing the TGFβ-1 gene appeared normal in their growth pattern. These findings indicate that high-level endogenous expression of TGFβ-1 in keratinocytes can at least inhibit, and possibly arrest, growth.
| Original language | English |
|---|---|
| Pages (from-to) | 1-6 |
| Number of pages | 6 |
| Journal | Journal of Dermatological Science |
| Volume | 27 |
| Issue number | 1 |
| State | Published - Jul 28 2001 |
| Externally published | Yes |
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Keywords
- Growth inhibition
- Keratinocyte
- TGFβ-1
ASJC Scopus subject areas
- Dermatology
Cite this
Growth inhibition of primary keratinocytes following transduction with a novel TGFβ-1 containing retrovirus. / Badiavas, Evangelos V; Zhou, Linda; Falanga, Vincent.
In: Journal of Dermatological Science, Vol. 27, No. 1, 28.07.2001, p. 1-6.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Growth inhibition of primary keratinocytes following transduction with a novel TGFβ-1 containing retrovirus
AU - Badiavas, Evangelos V
AU - Zhou, Linda
AU - Falanga, Vincent
PY - 2001/7/28
Y1 - 2001/7/28
N2 - Growth and migration of keratinocytes are known to be affected by the addition of exogenous cytokines, such as TGFβ-1, to culture media. We have developed a retroviral vector, LNTβ-1, that confers constitutive expression of human TGFβ-1 to transduced cells. Keratinocytes were exposed to retroviral particles generated in serum-free media, and infected cells were selected for with Geneticin. Transduced keratinocytes remained in culture as single cells instead of a normally grouped growth pattern. While these transduced keratinocytes survived in culture for several weeks, they did not proliferate and seemed arrested in their growth. Keratinocytes transduced with retrovirus not containing the TGFβ-1 gene appeared normal in their growth pattern. These findings indicate that high-level endogenous expression of TGFβ-1 in keratinocytes can at least inhibit, and possibly arrest, growth.
AB - Growth and migration of keratinocytes are known to be affected by the addition of exogenous cytokines, such as TGFβ-1, to culture media. We have developed a retroviral vector, LNTβ-1, that confers constitutive expression of human TGFβ-1 to transduced cells. Keratinocytes were exposed to retroviral particles generated in serum-free media, and infected cells were selected for with Geneticin. Transduced keratinocytes remained in culture as single cells instead of a normally grouped growth pattern. While these transduced keratinocytes survived in culture for several weeks, they did not proliferate and seemed arrested in their growth. Keratinocytes transduced with retrovirus not containing the TGFβ-1 gene appeared normal in their growth pattern. These findings indicate that high-level endogenous expression of TGFβ-1 in keratinocytes can at least inhibit, and possibly arrest, growth.
KW - Growth inhibition
KW - Keratinocyte
KW - TGFβ-1
UR - http://www.scopus.com/inward/record.url?scp=0034934218&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034934218&partnerID=8YFLogxK
M3 - Article
C2 - 11457637
AN - SCOPUS:0034934218
VL - 27
SP - 1
EP - 6
JO - Journal of Dermatological Science
JF - Journal of Dermatological Science
SN - 0923-1811
IS - 1
ER -