Growth Hormone-Releasing Hormone Receptor Splice Variant 1 is Frequently Expressed in Oral Squamous Cell Carcinomas

Nikolina Dioufa, Elena Farmaki, Andrew V. Schally, Hippokratis Kiaris, Dimitris Vlahodimitropoulos, Athanasios G. Papavassiliou, Christos Kittas, Norman L. Block, Ioulia Chatzistamou

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The expression of growth hormone-releasing hormone (GHRH) splice variant 1 (SV1) receptor in neoplastic lesions of the oral cavity was assessed. The sensitivity of HaCaT keratinocytes to GHRH analogs was also evaluated. Thirty-three benign precancerous oral lesions and 27 squamous cell carcinomas of the oral cavity were evaluated by immunohistochemistry for SV1 expression. SV1 expression in HaCaT keratinocytes was assessed by western blot. HaCaT proliferation was evaluated by cell counting. Anti-SV1 immunoreactivity was detected in only 9 % (three of 33) precancerous lesions (one hyperplasia and two dysplasias), while 44 % (12 of 27) carcinomas were positive for SV1 (p < 0.002). GHRH(1-29)NH 2 and GHRH agonist JI-38 stimulated HaCaT proliferation in vitro, and this effect was blocked by GHRH antagonists. These results indicate that SV1 expression may be associated with the transition of precancerous lesions to carcinomas of the oral epithelium. GHRH antagonists may be useful for the management of the disease.

Original languageEnglish (US)
Pages (from-to)172-180
Number of pages9
JournalHormones and Cancer
Volume3
Issue number4
DOIs
StatePublished - Aug 1 2012

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Oncology
  • Endocrinology
  • Endocrine and Autonomic Systems
  • Cancer Research

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    Dioufa, N., Farmaki, E., Schally, A. V., Kiaris, H., Vlahodimitropoulos, D., Papavassiliou, A. G., Kittas, C., Block, N. L., & Chatzistamou, I. (2012). Growth Hormone-Releasing Hormone Receptor Splice Variant 1 is Frequently Expressed in Oral Squamous Cell Carcinomas. Hormones and Cancer, 3(4), 172-180. https://doi.org/10.1007/s12672-012-0108-8