Growth factor treatment enhances vestibular hair cell renewal and results in improved vestibular function

Richard D. Kopke; Ronald L. Jackson; Geming Li; Mark D. Rasmussen; Michael E. Hoffer; Dorothy A. Frenz; Michael Costello; Peter Schultheiss; Thomas R. Van De Water

doi:10.1073/pnas.101120898

Growth factor treatment enhances vestibular hair cell renewal and results in improved vestibular function

Richard D. Kopke, Ronald L. Jackson, Geming Li, Mark D. Rasmussen, Michael E. Hoffer, Dorothy A. Frenz, Michael Costello, Peter Schultheiss, Thomas R. Van De Water

Otolaryngology

Research output: Contribution to journal › Article › peer-review

59 Scopus citations

Abstract

The vestibules of adult guinea pigs were lesioned with gentamicin and then treated with perilymphatic infusion of either of two growth factor mixtures (i.e., GF I or GF II). GF I contained transforming growth factor α (TGFα), insulin-like growth factor type one (IGF-1), and retinoic acid (RA), whereas GF II contained those three factors and brain-derived neurotrophic factor. Treatment with GF I significantly enhanced vestibular hair cell renewal in ototoxin-damaged utricles and the maturation of stereociliary bundle morphology. The addition of brain-derived neurotrophic factor to the GF II infusion mixture resulted in the return of type 1 vestibular hair cells in ototoxin-damaged cristae, and improved vestibular function. These results suggest that growth factor therapy may be an effective treatment for balance disorders that are the result of hair cell dysfunction and/or loss.

Original language	English (US)
Pages (from-to)	5886-5891
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	98
Issue number	10
DOIs	https://doi.org/10.1073/pnas.101120898
State	Published - May 8 2001

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Kopke, R. D., Jackson, R. L., Li, G., Rasmussen, M. D., Hoffer, M. E., Frenz, D. A., Costello, M., Schultheiss, P., & Van De Water, T. R. (2001). Growth factor treatment enhances vestibular hair cell renewal and results in improved vestibular function. Proceedings of the National Academy of Sciences of the United States of America, 98(10), 5886-5891. https://doi.org/10.1073/pnas.101120898

Growth factor treatment enhances vestibular hair cell renewal and results in improved vestibular function. / Kopke, Richard D.; Jackson, Ronald L.; Li, Geming; Rasmussen, Mark D.; Hoffer, Michael E.; Frenz, Dorothy A.; Costello, Michael; Schultheiss, Peter; Van De Water, Thomas R.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, No. 10, 08.05.2001, p. 5886-5891.

Research output: Contribution to journal › Article › peer-review

Kopke, RD, Jackson, RL, Li, G, Rasmussen, MD, Hoffer, ME, Frenz, DA, Costello, M, Schultheiss, P & Van De Water, TR 2001, 'Growth factor treatment enhances vestibular hair cell renewal and results in improved vestibular function', Proceedings of the National Academy of Sciences of the United States of America, vol. 98, no. 10, pp. 5886-5891. https://doi.org/10.1073/pnas.101120898

Kopke, Richard D. ; Jackson, Ronald L. ; Li, Geming ; Rasmussen, Mark D. ; Hoffer, Michael E. ; Frenz, Dorothy A. ; Costello, Michael ; Schultheiss, Peter ; Van De Water, Thomas R. / Growth factor treatment enhances vestibular hair cell renewal and results in improved vestibular function. In: Proceedings of the National Academy of Sciences of the United States of America. 2001 ; Vol. 98, No. 10. pp. 5886-5891.

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abstract = "The vestibules of adult guinea pigs were lesioned with gentamicin and then treated with perilymphatic infusion of either of two growth factor mixtures (i.e., GF I or GF II). GF I contained transforming growth factor α (TGFα), insulin-like growth factor type one (IGF-1), and retinoic acid (RA), whereas GF II contained those three factors and brain-derived neurotrophic factor. Treatment with GF I significantly enhanced vestibular hair cell renewal in ototoxin-damaged utricles and the maturation of stereociliary bundle morphology. The addition of brain-derived neurotrophic factor to the GF II infusion mixture resulted in the return of type 1 vestibular hair cells in ototoxin-damaged cristae, and improved vestibular function. These results suggest that growth factor therapy may be an effective treatment for balance disorders that are the result of hair cell dysfunction and/or loss.",

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AB - The vestibules of adult guinea pigs were lesioned with gentamicin and then treated with perilymphatic infusion of either of two growth factor mixtures (i.e., GF I or GF II). GF I contained transforming growth factor α (TGFα), insulin-like growth factor type one (IGF-1), and retinoic acid (RA), whereas GF II contained those three factors and brain-derived neurotrophic factor. Treatment with GF I significantly enhanced vestibular hair cell renewal in ototoxin-damaged utricles and the maturation of stereociliary bundle morphology. The addition of brain-derived neurotrophic factor to the GF II infusion mixture resulted in the return of type 1 vestibular hair cells in ototoxin-damaged cristae, and improved vestibular function. These results suggest that growth factor therapy may be an effective treatment for balance disorders that are the result of hair cell dysfunction and/or loss.

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