Growth cones are actively influenced by substrate-bound adhesion molecules

S. M. Burden-Gulley, H. R. Payne, Vance Lemmon

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

As axons advance to appropriate target tissues during development, their growth cones encounter a variety of cell adhesion molecules (CAMs) and extracellular matrix molecules (ECM molecules). Purified CAMs and ECM molecules influence neurite outgrowth in vitro and are thought to have a similar function in vivo. For example, when retinal ganglion cell (RGC) neurons are grown on different CAM and ECM molecule substrates in vitro, their growth cones display distinctive morphologies (Payne et al., 1992). Similarly, RGC growth cones in vivo have distinctive shapes at different points in the pathway from the eye to the tectum, suggesting the presence of localized cues that determine growth cone behaviors such as pathway selection at choice points. In this report, time-lapse video microscopy was utilized to examine dynamic transformations of RGC growth cones as they progressed from L1/8D9, N-cadherin, or laminin onto a different substrate. Contact made by the leading edge of a growth cone with a new substrate resulted in a rapid and dramatic alteration in growth cone morphology. In some cases, the changes encompassed the entire growth cone including those regions not in direct contact with the new substrate. In addition, the growth cones displayed a variety of behavioral responses that were dependent upon the order of substrate contact. These studies demonstrate that growth cones are actively affected by the substrate, and suggest that abrupt changes in the molecular composition of the growth cone environment are influential during axonal pathfinding.

Original languageEnglish
Pages (from-to)4370-4381
Number of pages12
JournalJournal of Neuroscience
Volume15
Issue number6
StatePublished - Jan 1 1995
Externally publishedYes

Fingerprint

Growth Cones
Retinal Ganglion Cells
Cell Adhesion Molecules
Extracellular Matrix
substrate adhesion molecules
Video Microscopy
Laminin
Cadherins
Cues
Axons

Keywords

  • growth cone
  • L1
  • laminin
  • N-cadherin
  • pathfinding
  • retinal ganglion cell

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Growth cones are actively influenced by substrate-bound adhesion molecules. / Burden-Gulley, S. M.; Payne, H. R.; Lemmon, Vance.

In: Journal of Neuroscience, Vol. 15, No. 6, 01.01.1995, p. 4370-4381.

Research output: Contribution to journalArticle

Burden-Gulley, S. M. ; Payne, H. R. ; Lemmon, Vance. / Growth cones are actively influenced by substrate-bound adhesion molecules. In: Journal of Neuroscience. 1995 ; Vol. 15, No. 6. pp. 4370-4381.
@article{7e4ece6bb96a4981b7dcc784b57219df,
title = "Growth cones are actively influenced by substrate-bound adhesion molecules",
abstract = "As axons advance to appropriate target tissues during development, their growth cones encounter a variety of cell adhesion molecules (CAMs) and extracellular matrix molecules (ECM molecules). Purified CAMs and ECM molecules influence neurite outgrowth in vitro and are thought to have a similar function in vivo. For example, when retinal ganglion cell (RGC) neurons are grown on different CAM and ECM molecule substrates in vitro, their growth cones display distinctive morphologies (Payne et al., 1992). Similarly, RGC growth cones in vivo have distinctive shapes at different points in the pathway from the eye to the tectum, suggesting the presence of localized cues that determine growth cone behaviors such as pathway selection at choice points. In this report, time-lapse video microscopy was utilized to examine dynamic transformations of RGC growth cones as they progressed from L1/8D9, N-cadherin, or laminin onto a different substrate. Contact made by the leading edge of a growth cone with a new substrate resulted in a rapid and dramatic alteration in growth cone morphology. In some cases, the changes encompassed the entire growth cone including those regions not in direct contact with the new substrate. In addition, the growth cones displayed a variety of behavioral responses that were dependent upon the order of substrate contact. These studies demonstrate that growth cones are actively affected by the substrate, and suggest that abrupt changes in the molecular composition of the growth cone environment are influential during axonal pathfinding.",
keywords = "growth cone, L1, laminin, N-cadherin, pathfinding, retinal ganglion cell",
author = "Burden-Gulley, {S. M.} and Payne, {H. R.} and Vance Lemmon",
year = "1995",
month = "1",
day = "1",
language = "English",
volume = "15",
pages = "4370--4381",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "6",

}

TY - JOUR

T1 - Growth cones are actively influenced by substrate-bound adhesion molecules

AU - Burden-Gulley, S. M.

AU - Payne, H. R.

AU - Lemmon, Vance

PY - 1995/1/1

Y1 - 1995/1/1

N2 - As axons advance to appropriate target tissues during development, their growth cones encounter a variety of cell adhesion molecules (CAMs) and extracellular matrix molecules (ECM molecules). Purified CAMs and ECM molecules influence neurite outgrowth in vitro and are thought to have a similar function in vivo. For example, when retinal ganglion cell (RGC) neurons are grown on different CAM and ECM molecule substrates in vitro, their growth cones display distinctive morphologies (Payne et al., 1992). Similarly, RGC growth cones in vivo have distinctive shapes at different points in the pathway from the eye to the tectum, suggesting the presence of localized cues that determine growth cone behaviors such as pathway selection at choice points. In this report, time-lapse video microscopy was utilized to examine dynamic transformations of RGC growth cones as they progressed from L1/8D9, N-cadherin, or laminin onto a different substrate. Contact made by the leading edge of a growth cone with a new substrate resulted in a rapid and dramatic alteration in growth cone morphology. In some cases, the changes encompassed the entire growth cone including those regions not in direct contact with the new substrate. In addition, the growth cones displayed a variety of behavioral responses that were dependent upon the order of substrate contact. These studies demonstrate that growth cones are actively affected by the substrate, and suggest that abrupt changes in the molecular composition of the growth cone environment are influential during axonal pathfinding.

AB - As axons advance to appropriate target tissues during development, their growth cones encounter a variety of cell adhesion molecules (CAMs) and extracellular matrix molecules (ECM molecules). Purified CAMs and ECM molecules influence neurite outgrowth in vitro and are thought to have a similar function in vivo. For example, when retinal ganglion cell (RGC) neurons are grown on different CAM and ECM molecule substrates in vitro, their growth cones display distinctive morphologies (Payne et al., 1992). Similarly, RGC growth cones in vivo have distinctive shapes at different points in the pathway from the eye to the tectum, suggesting the presence of localized cues that determine growth cone behaviors such as pathway selection at choice points. In this report, time-lapse video microscopy was utilized to examine dynamic transformations of RGC growth cones as they progressed from L1/8D9, N-cadherin, or laminin onto a different substrate. Contact made by the leading edge of a growth cone with a new substrate resulted in a rapid and dramatic alteration in growth cone morphology. In some cases, the changes encompassed the entire growth cone including those regions not in direct contact with the new substrate. In addition, the growth cones displayed a variety of behavioral responses that were dependent upon the order of substrate contact. These studies demonstrate that growth cones are actively affected by the substrate, and suggest that abrupt changes in the molecular composition of the growth cone environment are influential during axonal pathfinding.

KW - growth cone

KW - L1

KW - laminin

KW - N-cadherin

KW - pathfinding

KW - retinal ganglion cell

UR - http://www.scopus.com/inward/record.url?scp=0029034002&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029034002&partnerID=8YFLogxK

M3 - Article

VL - 15

SP - 4370

EP - 4381

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 6

ER -