Growth cone pathfinding and filopodial dynamics are mediated separately by Cdc42 activation

Michael D. Kim, Peter Kolodziej, Akira Chiba

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Although evidence exists that activation of the Rho family GTPase Cdc42 affects axonal development, its specific roles within a growth cone are not well delineated. To evaluate the model that Cdc42 activation regulates growth cone navigation by promoting filopodial activity, we adopted a live analysis strategy that uses transgenic Drosophila lines in which neurons coexpressed constitutively active Cdc42 (Cdc42V12) and membrane-targeted green fluorescent protein. We found that growth cones that displayed pathfinding defects exhibited little change in their filopodial activity, whereas others without pathfinding defects exhibited an ∼50% increase in their filopodial activity. Moreover, effector loop mutations that were added to the constitutively active Cdc42 (Cdc42V12C40 and Cdc42V12A37) exerted little influence over filopodial activity caused by Cdc42 activation but suppressed the pathfinding defects of the growth cones. Together, these data suggest that Cdc42 controls filopodial activity in axonal growth cones independently of its effects on their pathfinding.

Original languageEnglish (US)
Pages (from-to)1794-1806
Number of pages13
JournalJournal of Neuroscience
Volume22
Issue number5
StatePublished - Mar 1 2002

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Keywords

  • Axon guidance
  • Cdc42
  • Drosophila
  • Filopodia
  • GFP
  • Growth cone
  • In vivo
  • Pathfinding

ASJC Scopus subject areas

  • Neuroscience(all)

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