Growth cone interactions with purified cell and substrate adhesion molecules visualized by interference reflection microscopy

Judith Drazba, Patricia Liljelund, Carolyn Smith, Ross Payne, Vance Lemmon

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The migration of growth cones on substrates consisting of naturally occurring cell adhesion molecules has been extensively studied in cell culture. However, relatively little is known about how growth cones contact the substrate or how the patterns of contact change as growth cones move forward. We have examined the interactions of chick retinal ganglion cell growth cones with laminin, merosin, N-cadherin, L1 and poly-L-lysine by time- lapse interference reflection microscopy (IRM) using a laser scanning confocal microscope. In images obtained by IRM, areas of a cell that are closely apposed to the substrate appear dark whereas areas that are farther away appear light. Growth cones on laminin and merosin were almost uniformly light, indicating that very little of the membrane was in close contact with the substrate. Growth cones on N-cadherin had a mottled appearance with some relatively large dark gray areas. The proximal portions of filopodia often were dark, in contrast to those on laminin and merosin which were light. In addition, growth cones on N-cadherin had numerous dark gray punctate regions of close association with the substrate. Growth cones on L1 had darker regions than growth cones on other substrates and these comprised a larger fraction of their area. There also were differences in the temporal dynamics of growth cone interactions with different substrates and these differences correlated with differences in rates of growth. None of the contacts observed in growth cones were as dark or stable as focal contacts of fibroblasts.

Original languageEnglish
Pages (from-to)183-197
Number of pages15
JournalDevelopmental Brain Research
Volume100
Issue number2
DOIs
StatePublished - Jun 18 1997
Externally publishedYes

Fingerprint

Interference Microscopy
Growth Cones
Cell Adhesion Molecules
Laminin
Cadherins
Light
substrate adhesion molecules
Pseudopodia
Focal Adhesions
Retinal Ganglion Cells
Lysine
Lasers

Keywords

  • Adhesion molecule
  • Growth cone
  • Interference reflection microscopy
  • Neurite growth
  • Time-lapse video microscopy

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

Growth cone interactions with purified cell and substrate adhesion molecules visualized by interference reflection microscopy. / Drazba, Judith; Liljelund, Patricia; Smith, Carolyn; Payne, Ross; Lemmon, Vance.

In: Developmental Brain Research, Vol. 100, No. 2, 18.06.1997, p. 183-197.

Research output: Contribution to journalArticle

Drazba, Judith ; Liljelund, Patricia ; Smith, Carolyn ; Payne, Ross ; Lemmon, Vance. / Growth cone interactions with purified cell and substrate adhesion molecules visualized by interference reflection microscopy. In: Developmental Brain Research. 1997 ; Vol. 100, No. 2. pp. 183-197.
@article{4d68c5d8258e43daa53c0c117172ea6e,
title = "Growth cone interactions with purified cell and substrate adhesion molecules visualized by interference reflection microscopy",
abstract = "The migration of growth cones on substrates consisting of naturally occurring cell adhesion molecules has been extensively studied in cell culture. However, relatively little is known about how growth cones contact the substrate or how the patterns of contact change as growth cones move forward. We have examined the interactions of chick retinal ganglion cell growth cones with laminin, merosin, N-cadherin, L1 and poly-L-lysine by time- lapse interference reflection microscopy (IRM) using a laser scanning confocal microscope. In images obtained by IRM, areas of a cell that are closely apposed to the substrate appear dark whereas areas that are farther away appear light. Growth cones on laminin and merosin were almost uniformly light, indicating that very little of the membrane was in close contact with the substrate. Growth cones on N-cadherin had a mottled appearance with some relatively large dark gray areas. The proximal portions of filopodia often were dark, in contrast to those on laminin and merosin which were light. In addition, growth cones on N-cadherin had numerous dark gray punctate regions of close association with the substrate. Growth cones on L1 had darker regions than growth cones on other substrates and these comprised a larger fraction of their area. There also were differences in the temporal dynamics of growth cone interactions with different substrates and these differences correlated with differences in rates of growth. None of the contacts observed in growth cones were as dark or stable as focal contacts of fibroblasts.",
keywords = "Adhesion molecule, Growth cone, Interference reflection microscopy, Neurite growth, Time-lapse video microscopy",
author = "Judith Drazba and Patricia Liljelund and Carolyn Smith and Ross Payne and Vance Lemmon",
year = "1997",
month = "6",
day = "18",
doi = "10.1016/S0165-3806(97)00041-2",
language = "English",
volume = "100",
pages = "183--197",
journal = "Developmental Brain Research",
issn = "0165-3806",
publisher = "Elsevier BV",
number = "2",

}

TY - JOUR

T1 - Growth cone interactions with purified cell and substrate adhesion molecules visualized by interference reflection microscopy

AU - Drazba, Judith

AU - Liljelund, Patricia

AU - Smith, Carolyn

AU - Payne, Ross

AU - Lemmon, Vance

PY - 1997/6/18

Y1 - 1997/6/18

N2 - The migration of growth cones on substrates consisting of naturally occurring cell adhesion molecules has been extensively studied in cell culture. However, relatively little is known about how growth cones contact the substrate or how the patterns of contact change as growth cones move forward. We have examined the interactions of chick retinal ganglion cell growth cones with laminin, merosin, N-cadherin, L1 and poly-L-lysine by time- lapse interference reflection microscopy (IRM) using a laser scanning confocal microscope. In images obtained by IRM, areas of a cell that are closely apposed to the substrate appear dark whereas areas that are farther away appear light. Growth cones on laminin and merosin were almost uniformly light, indicating that very little of the membrane was in close contact with the substrate. Growth cones on N-cadherin had a mottled appearance with some relatively large dark gray areas. The proximal portions of filopodia often were dark, in contrast to those on laminin and merosin which were light. In addition, growth cones on N-cadherin had numerous dark gray punctate regions of close association with the substrate. Growth cones on L1 had darker regions than growth cones on other substrates and these comprised a larger fraction of their area. There also were differences in the temporal dynamics of growth cone interactions with different substrates and these differences correlated with differences in rates of growth. None of the contacts observed in growth cones were as dark or stable as focal contacts of fibroblasts.

AB - The migration of growth cones on substrates consisting of naturally occurring cell adhesion molecules has been extensively studied in cell culture. However, relatively little is known about how growth cones contact the substrate or how the patterns of contact change as growth cones move forward. We have examined the interactions of chick retinal ganglion cell growth cones with laminin, merosin, N-cadherin, L1 and poly-L-lysine by time- lapse interference reflection microscopy (IRM) using a laser scanning confocal microscope. In images obtained by IRM, areas of a cell that are closely apposed to the substrate appear dark whereas areas that are farther away appear light. Growth cones on laminin and merosin were almost uniformly light, indicating that very little of the membrane was in close contact with the substrate. Growth cones on N-cadherin had a mottled appearance with some relatively large dark gray areas. The proximal portions of filopodia often were dark, in contrast to those on laminin and merosin which were light. In addition, growth cones on N-cadherin had numerous dark gray punctate regions of close association with the substrate. Growth cones on L1 had darker regions than growth cones on other substrates and these comprised a larger fraction of their area. There also were differences in the temporal dynamics of growth cone interactions with different substrates and these differences correlated with differences in rates of growth. None of the contacts observed in growth cones were as dark or stable as focal contacts of fibroblasts.

KW - Adhesion molecule

KW - Growth cone

KW - Interference reflection microscopy

KW - Neurite growth

KW - Time-lapse video microscopy

UR - http://www.scopus.com/inward/record.url?scp=0030799492&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030799492&partnerID=8YFLogxK

U2 - 10.1016/S0165-3806(97)00041-2

DO - 10.1016/S0165-3806(97)00041-2

M3 - Article

C2 - 9205809

AN - SCOPUS:0030799492

VL - 100

SP - 183

EP - 197

JO - Developmental Brain Research

JF - Developmental Brain Research

SN - 0165-3806

IS - 2

ER -