Growth and recombination of phage λ in the presence of exonuclease V from Bacillus subtilis

F. Stahl, R. Bowers, D. Mooney, R. Myers, M. Stahl, L. Thomason

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

When expressed in Escherichia coli, the AddAB exonuclease/recombinase from Bacillus subtilis blocks the growth of phage λ. Mutants of λ that are deleted for ea47, a gene of unknown function which is expressed early in the lytic cycle, are not blocked for growth. The blocked-growth phenotype of λ ea47+ in the presence of AddAB is expressed only when phage DNA replication is permitted.

Original languageEnglish (US)
Pages (from-to)716-723
Number of pages8
JournalMolecular and General Genetics
Volume264
Issue number5
DOIs
StatePublished - Feb 6 2001

Keywords

  • AddAB
  • Bacteriophage λ
  • Ea47 gene
  • Recombination

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Growth and recombination of phage λ in the presence of exonuclease V from Bacillus subtilis'. Together they form a unique fingerprint.

  • Cite this