Abstract
When expressed in Escherichia coli, the AddAB exonuclease/recombinase from Bacillus subtilis blocks the growth of phage λ. Mutants of λ that are deleted for ea47, a gene of unknown function which is expressed early in the lytic cycle, are not blocked for growth. The blocked-growth phenotype of λ ea47+ in the presence of AddAB is expressed only when phage DNA replication is permitted.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 716-723 |
| Number of pages | 8 |
| Journal | Molecular and General Genetics |
| Volume | 264 |
| Issue number | 5 |
| DOIs | |
| State | Published - Feb 6 2001 |
Keywords
- AddAB
- Bacteriophage λ
- Ea47 gene
- Recombination
ASJC Scopus subject areas
- Genetics
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Stahl, F., Bowers, R., Mooney, D., Myers, R., Stahl, M., & Thomason, L. (2001). Growth and recombination of phage λ in the presence of exonuclease V from Bacillus subtilis. Molecular and General Genetics, 264(5), 716-723. https://doi.org/10.1007/s004380000361