Growth and recombination of phage λ in the presence of exonuclease V from Bacillus subtilis

F. Stahl, R. Bowers, D. Mooney, Richard Myers, M. Stahl, L. Thomason

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

When expressed in Escherichia coli, the AddAB exonuclease/recombinase from Bacillus subtilis blocks the growth of phage λ. Mutants of λ that are deleted for ea47, a gene of unknown function which is expressed early in the lytic cycle, are not blocked for growth. The blocked-growth phenotype of λ ea47+ in the presence of AddAB is expressed only when phage DNA replication is permitted.

Original languageEnglish (US)
Pages (from-to)716-723
Number of pages8
JournalMolecular and General Genetics
Volume264
Issue number5
DOIs
StatePublished - Feb 6 2001

Fingerprint

Exodeoxyribonuclease V
Bacillus subtilis
Bacteriophages
Genetic Recombination
Growth
Exonucleases
Recombinases
DNA Replication
Escherichia coli
Phenotype
Genes

Keywords

  • AddAB
  • Bacteriophage λ
  • Ea47 gene
  • Recombination

ASJC Scopus subject areas

  • Genetics

Cite this

Growth and recombination of phage λ in the presence of exonuclease V from Bacillus subtilis. / Stahl, F.; Bowers, R.; Mooney, D.; Myers, Richard; Stahl, M.; Thomason, L.

In: Molecular and General Genetics, Vol. 264, No. 5, 06.02.2001, p. 716-723.

Research output: Contribution to journalArticle

Stahl, F. ; Bowers, R. ; Mooney, D. ; Myers, Richard ; Stahl, M. ; Thomason, L. / Growth and recombination of phage λ in the presence of exonuclease V from Bacillus subtilis. In: Molecular and General Genetics. 2001 ; Vol. 264, No. 5. pp. 716-723.
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