Abstract
When expressed in Escherichia coli, the AddAB exonuclease/recombinase from Bacillus subtilis blocks the growth of phage λ. Mutants of λ that are deleted for ea47, a gene of unknown function which is expressed early in the lytic cycle, are not blocked for growth. The blocked-growth phenotype of λ ea47+ in the presence of AddAB is expressed only when phage DNA replication is permitted.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 716-723 |
| Number of pages | 8 |
| Journal | Molecular and General Genetics |
| Volume | 264 |
| Issue number | 5 |
| DOIs | |
| State | Published - Feb 6 2001 |
Fingerprint
Keywords
- AddAB
- Bacteriophage λ
- Ea47 gene
- Recombination
ASJC Scopus subject areas
- Genetics
Cite this
Growth and recombination of phage λ in the presence of exonuclease V from Bacillus subtilis. / Stahl, F.; Bowers, R.; Mooney, D.; Myers, Richard; Stahl, M.; Thomason, L.
In: Molecular and General Genetics, Vol. 264, No. 5, 06.02.2001, p. 716-723.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Growth and recombination of phage λ in the presence of exonuclease V from Bacillus subtilis
AU - Stahl, F.
AU - Bowers, R.
AU - Mooney, D.
AU - Myers, Richard
AU - Stahl, M.
AU - Thomason, L.
PY - 2001/2/6
Y1 - 2001/2/6
N2 - When expressed in Escherichia coli, the AddAB exonuclease/recombinase from Bacillus subtilis blocks the growth of phage λ. Mutants of λ that are deleted for ea47, a gene of unknown function which is expressed early in the lytic cycle, are not blocked for growth. The blocked-growth phenotype of λ ea47+ in the presence of AddAB is expressed only when phage DNA replication is permitted.
AB - When expressed in Escherichia coli, the AddAB exonuclease/recombinase from Bacillus subtilis blocks the growth of phage λ. Mutants of λ that are deleted for ea47, a gene of unknown function which is expressed early in the lytic cycle, are not blocked for growth. The blocked-growth phenotype of λ ea47+ in the presence of AddAB is expressed only when phage DNA replication is permitted.
KW - AddAB
KW - Bacteriophage λ
KW - Ea47 gene
KW - Recombination
UR - http://www.scopus.com/inward/record.url?scp=0035145390&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035145390&partnerID=8YFLogxK
U2 - 10.1007/s004380000361
DO - 10.1007/s004380000361
M3 - Article
C2 - 11212927
AN - SCOPUS:0035145390
VL - 264
SP - 716
EP - 723
JO - Molecular Genetics and Genomics
JF - Molecular Genetics and Genomics
SN - 1617-4615
IS - 5
ER -