Green tea polyphenols induce apoptosis in vitro in peripheral blood T lymphocytes of adult T-cell leukemia patients

Hong Chuan Li, Shinji Yashiki, Junichiro Sonoda, Hong Lou, Subrata K. Ghosh, John Byrnes, Carolina Lema, Toshinobu Fujiyoshi, Mitsuaki Karasuyama, Shunro Sonoda

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Abstract

Green tea polyphenols (TEA) are known to exhibit antioxidative activity as well as tumor-suppressing activity. In order to examine the tumor-suppressing activity of TEA against adult T-cell leukemia (ATL), we cultivated peripheral blood T lymphocytes of ATL patients (ATL PBLs), an HTLV-I-infected T-cell line (KODV) and healthy controls (normal PBLs) for 3 days in the presence of TEA and its main constituent, epigallocatechin-3-gallate (EGCg), to measure cell proliferation and apoptosis, and to quantitate mRNAs of HTLV-I pX and β-actin genes of the cultured cells. Growth of ATL PBLs was significantly inhibited by 9-27 μg/ml of TEA and EGCg, in contrast to minimal growth inhibition of T cells of normal PBLs. Inhibition of KODV was intermediate between ATL PBLs and normal PBLs. The ATL PBLs and KODV treated with 27 μg/ml of either TEA or EGCg induced apoptotic DNA fragmentation, producing terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL)-positive cells, while the normal PBLs treated with the same concentration of TEA or EGCg produced a negligibly small number of TUNEL-positive cells, in which apoptotic DNA fragmentation was not detectable. Expression of HTLV-I pX mRNA was suppressed more than 90% in ATL PBLs by treatment with 3-27 μg/ml of either TEA or EGCg, while expression of β-actin mRNA was much less suppressed by treatment with the same concentration of TEA or EGCg. These results indicate that TEA and EGCg inhibit growth of ATL PBLs, as well as HTLV-I-infected T-cells, by suppressing HTLV-I pX gene expression and inducing apoptotic cell death.

Original languageEnglish
Pages (from-to)34-40
Number of pages7
JournalJapanese Journal of Cancer Research
Volume91
Issue number1
StatePublished - Jan 1 2000

Fingerprint

Adult T Cell Leukemia Lymphoma
Polyphenols
Tea
Human T-lymphotropic virus 1
Apoptosis
T-Lymphocytes
pX Genes
DNA Fragmentation
Biotin
Messenger RNA
Actins
Growth
DNA Nucleotidylexotransferase
In Vitro Techniques
Transferases
epigallocatechin gallate
Cultured Cells
Neoplasms
Cell Death
Cell Proliferation

Keywords

  • Apoptosis
  • ATL
  • EGCg
  • Green tea
  • HTLV-I

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Green tea polyphenols induce apoptosis in vitro in peripheral blood T lymphocytes of adult T-cell leukemia patients. / Li, Hong Chuan; Yashiki, Shinji; Sonoda, Junichiro; Lou, Hong; Ghosh, Subrata K.; Byrnes, John; Lema, Carolina; Fujiyoshi, Toshinobu; Karasuyama, Mitsuaki; Sonoda, Shunro.

In: Japanese Journal of Cancer Research, Vol. 91, No. 1, 01.01.2000, p. 34-40.

Research output: Contribution to journalArticle

Li, HC, Yashiki, S, Sonoda, J, Lou, H, Ghosh, SK, Byrnes, J, Lema, C, Fujiyoshi, T, Karasuyama, M & Sonoda, S 2000, 'Green tea polyphenols induce apoptosis in vitro in peripheral blood T lymphocytes of adult T-cell leukemia patients', Japanese Journal of Cancer Research, vol. 91, no. 1, pp. 34-40.
Li, Hong Chuan ; Yashiki, Shinji ; Sonoda, Junichiro ; Lou, Hong ; Ghosh, Subrata K. ; Byrnes, John ; Lema, Carolina ; Fujiyoshi, Toshinobu ; Karasuyama, Mitsuaki ; Sonoda, Shunro. / Green tea polyphenols induce apoptosis in vitro in peripheral blood T lymphocytes of adult T-cell leukemia patients. In: Japanese Journal of Cancer Research. 2000 ; Vol. 91, No. 1. pp. 34-40.
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abstract = "Green tea polyphenols (TEA) are known to exhibit antioxidative activity as well as tumor-suppressing activity. In order to examine the tumor-suppressing activity of TEA against adult T-cell leukemia (ATL), we cultivated peripheral blood T lymphocytes of ATL patients (ATL PBLs), an HTLV-I-infected T-cell line (KODV) and healthy controls (normal PBLs) for 3 days in the presence of TEA and its main constituent, epigallocatechin-3-gallate (EGCg), to measure cell proliferation and apoptosis, and to quantitate mRNAs of HTLV-I pX and β-actin genes of the cultured cells. Growth of ATL PBLs was significantly inhibited by 9-27 μg/ml of TEA and EGCg, in contrast to minimal growth inhibition of T cells of normal PBLs. Inhibition of KODV was intermediate between ATL PBLs and normal PBLs. The ATL PBLs and KODV treated with 27 μg/ml of either TEA or EGCg induced apoptotic DNA fragmentation, producing terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL)-positive cells, while the normal PBLs treated with the same concentration of TEA or EGCg produced a negligibly small number of TUNEL-positive cells, in which apoptotic DNA fragmentation was not detectable. Expression of HTLV-I pX mRNA was suppressed more than 90{\%} in ATL PBLs by treatment with 3-27 μg/ml of either TEA or EGCg, while expression of β-actin mRNA was much less suppressed by treatment with the same concentration of TEA or EGCg. These results indicate that TEA and EGCg inhibit growth of ATL PBLs, as well as HTLV-I-infected T-cells, by suppressing HTLV-I pX gene expression and inducing apoptotic cell death.",
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AU - Ghosh, Subrata K.

AU - Byrnes, John

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