GREBI is a novel androgen-regulated gene required for prostate cancer growth

James M. Rae, Michael D. Johnson, Kevin E. Cordero, Joshua O. Scheys, José M. Larios, Marco M. Gottardis, Kenneth J. Pienta, Marc E Lippman

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

BACKGROUND. Gene regulated in breast cancer 1 (GREB1) is a novel estrogen-regulated gene shown to play a pivotal role in hormone-stimulated breast cancer growth. GREB1 is expressed in the prostate and its putative promoter contains potential androgen receptor (AR) response elements. METHODS. We investigated the effects of androgens on GREB1 expression and its role in androgen-dependent prostate cancer growth. RESULTS. Real-time PCR demonstrated high level GREB1 expression in benign prostatic hypertrophy (BPH), localized prostate cancer (L-PCa), and hormone refractory prostate cancer (HR-PCa). Androgen treatment of AR-positive prostate cancer cells induced dose-dependent GREB1 expression, which was blocked by anti-androgens. AR binding to the GREB1 promoter was confirmed by chromatin immunoprecipitation (ChIP) assays. Suppression of GREB1 by RNA interference blocked androgen-stimulated LNCaP cell proliferation. CONCLUSIONS. GREB1 is expressed in proliferating prostatic tissue and prostate cancer, is regulated by androgens, and suppression of GREB1 blocks androgen-induced growth suggesting GREB1 may be critically involved in prostate cancer proliferation.

Original languageEnglish
Pages (from-to)886-894
Number of pages9
JournalProstate
Volume66
Issue number8
DOIs
StatePublished - Jun 1 2006
Externally publishedYes

Fingerprint

Androgens
Prostatic Neoplasms
Breast Neoplasms
Growth
Genes
Androgen Receptors
Hormones
Chromatin Immunoprecipitation
Prostatic Hyperplasia
Response Elements
RNA Interference
Real-Time Polymerase Chain Reaction
Prostate
Estrogens
Cell Proliferation

Keywords

  • Androgen receptor
  • Androgen-induced growth
  • GREB1
  • Prostate cancer

ASJC Scopus subject areas

  • Urology

Cite this

Rae, J. M., Johnson, M. D., Cordero, K. E., Scheys, J. O., Larios, J. M., Gottardis, M. M., ... Lippman, M. E. (2006). GREBI is a novel androgen-regulated gene required for prostate cancer growth. Prostate, 66(8), 886-894. https://doi.org/10.1002/pros.20403

GREBI is a novel androgen-regulated gene required for prostate cancer growth. / Rae, James M.; Johnson, Michael D.; Cordero, Kevin E.; Scheys, Joshua O.; Larios, José M.; Gottardis, Marco M.; Pienta, Kenneth J.; Lippman, Marc E.

In: Prostate, Vol. 66, No. 8, 01.06.2006, p. 886-894.

Research output: Contribution to journalArticle

Rae, JM, Johnson, MD, Cordero, KE, Scheys, JO, Larios, JM, Gottardis, MM, Pienta, KJ & Lippman, ME 2006, 'GREBI is a novel androgen-regulated gene required for prostate cancer growth', Prostate, vol. 66, no. 8, pp. 886-894. https://doi.org/10.1002/pros.20403
Rae JM, Johnson MD, Cordero KE, Scheys JO, Larios JM, Gottardis MM et al. GREBI is a novel androgen-regulated gene required for prostate cancer growth. Prostate. 2006 Jun 1;66(8):886-894. https://doi.org/10.1002/pros.20403
Rae, James M. ; Johnson, Michael D. ; Cordero, Kevin E. ; Scheys, Joshua O. ; Larios, José M. ; Gottardis, Marco M. ; Pienta, Kenneth J. ; Lippman, Marc E. / GREBI is a novel androgen-regulated gene required for prostate cancer growth. In: Prostate. 2006 ; Vol. 66, No. 8. pp. 886-894.
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